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Biomedical subjects

N Prasad

Publications and source records attributed to N Prasad.

At least 109 records · Page 6Linked to original sources
PubMed1978

Variation in the lactase dehydrogenase activity of the esophagus.

Quantitative assay and electrophoretic study of lactate dehydrogenase (LDH) from various tissues of the opossum esophagus were performed. On the basis of expression of the LDH isozymes, we concluded that the smooth muscle of the body of the esophagus carry on more anaerobic glycolysis than the striated muscle. The smooth muscle of the gastroesophageal junction carry on both anaerobic as well aerobic glycolysis.

Animals↗
PubMed1978

Effect of maternal alcohol exposure on fetal ovarian lactate dehydrogenase.

Pregnant inbred mice strain Balb/c injected intraperitoneally with 100 mu1 of 70% ethanol for 7 consecutive days (9th through 16th day of gestation). One group of mice were also injected with diethylstibestrol (DES) dissolved in 70% ethanol. The lactate dehydrogenase (LDH) activity was measured 90 days postpartum in the crude extracts of ovarian tissues (obtained from mothers and offspring) as well as in testicular tissues of the male offspring. The data show that the electrophoretic pattern of LDH in these tissues does not change, but the ovarian LDH is inhibited by alcohol. The testicular LDH on the other hand is not affected by the alcohol treatment. Because of the effect of alcohol itself, the effect of DES could not be determined.

Animals↗
PubMed1977

Effect of irradiation on electrophoretic properties of enzymes in haemopoietic cells of opossum.

5-month-old male opossums were exposed to 5000 rads whole body 60Co radiation and sacrificed at 16, 40 and 90 h following irradiation. Stract gel electrophoretic studies of dehydrogenases of lactate, malate, 6-phospho-gluconate and glucose-6-phosphate and alpha-esterases and acid phosphatase were conducted on the homogenates of spleen and bone marrow cells. Expressions of LDH and G6PD were affected by irradiation in bone marrow and spleen cells.

Animals↗
PubMed1977

Effect of irradiation on testicular cells of opossum.

Five months old male opossums were exposed to 5000 rd wholebody 60Co gamma-radiation. Testes tissues from animals sacrificed at 16, 40 and 90 hours post-irradiation and from nonirradiated animales were used for enzymatic and histological studies. Electrophoretic pattern of lactate dehydrogenase and glucose-6-phosphate dehydrogenase was slightly disturbed in early hours in irradiated animals, but it did not persist beyond 40 hours postirradiation. Histological study indicates 31% survival of type A spermatogonia suggesting high radioresistance of testes tissue in comparison to other animals.

Animals↗
PubMed1977

Induction of hepatic aryl hydrocarbon hydroxylase in C57BL mice by ionizing radiation.

Inbred strains of C57BL mice were exposed to either 100, 500, or 1000 rads of whole-body irradiation. Another group of mice were given injections of 3-methylcholanthrene only, and a control group had no treatment. Forty-eight hr after treatment, the animals were killed and hepatic aryl hydrocarbon hydroxylase (AHH) activity was measured by spectrophotofluorometer. A comparison of hepatic AHH activity in treated and nontreated groups of mice showed that each treated group had a significantly increased AHH induction compared with the control group. Although radiation appeared to have a dose-related effect on AHH induction, the increase with dose level was not statistically significant.

Animals↗
PubMed1976

Altered enzyme expression in "differentiated" murine neuroblastoma cells.

Out of 17 enzymes studied, only 9 were detectable by starch gel electrophoresis in mouse neuroblastoma cells in culture. Prostaglandin E1 (PGE1) and 4(-3-butoxy-4-methoxybenzyl)-2-imidazolidinone (R020-1724), a specific inhibitor of cAMP phosphodiesterase, were used to induce "differentiation". Lactate and 6-phosphogluconate dehydrogenases and adenylate kinase were expressed as single bands in untreated neuroblastoma and induced "differentiated" cells, but the electrophoretic mobility of these enzymes in PGE1-treated cells was slower than that in malignant and R020-1724-treated cells. Three bands of glucose 6-phosphate dehydrogenase were detectable in PGE1-treated cells, whereas the R020-1724-treated cells had two bands and the untreated neuroblastoma cells had only one band. Aldolase was also expressed as a single band; however, the activity of this enzyme was much higher in PGE1-treated cells, whereas the activity was bately detectable for R020-1724-treated and untreated neuroblastoma cells. Some of the enzymes which are present in vivo are absent in vitro. Alkaline phosphatase is present in brain but is absent in neuroblastoma cells in vivo and in vitro. Two bands each of triose phsophate isomerase, fumarase and aldolase are present in brain, but only one band of these enzymes is present in neuroblastoma cells. Although PGE1 and R020-1724 induce many differentiated functions in neuroblastoma cells in a similar manner, PGE1 appears to change characteristically the expression of several enzymes.

4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone↗
PubMed1973

Esterase, malate, and lactate dehydrogenases activity in murine neuroblastoma.

Mouse neuroblastoma tumors have only the fifth isozyme band (A(4)) of lactate dehydrogenase, whereas this band is missing in the brain which contained four other bands of lactate dehydrogenase. The alpha-esterase isozyme patterns of tumors, kidney, and brain are similar except that there is an additional slowest-moving form of esterase in all tumor tissues. The malate dehydrogenase pattern is not altered in any of the tissues.

Animals↗