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Biomedical subjects

N P Korokhov

Publications and source records attributed to N P Korokhov.

8 recordsLinked to original sources
PubMed1996

[Characterization of rat lines with normotensive and hypertensive status using genomic fingerprinting].

The properties of normotensive and hypertensive rat lines were investigated by the DNA fingerprinting method using a multilocus micro-satellite (CAC)5 probe. The HaeIII and HinfI restriction endonucleases were found to be the most informative enzymes in this case. The high genetic homogeneity of the ISIAH line, a rat line with inherited stress-sensitive arterial hypertension created at the Institute of Cytology and Genetics, was demonstrated. The lack of intralinear polymorphism was also typical to Japanese SHR rats with spontaneous arterial hypertension. Normotensive WAG rats had identical fingerprinting patterns, while the relative intensity of some bands was different. The outbred normotensive Wistar line maintained at the Institute, appeared to carry 30% polymorphic alleles. Hypertensive lines differed from the normotensive by a number of genetic markers.

Animals↗
PubMed1986

[Nature of the endogenous retrovirus-like particles of the rat liver].

Previously virus-like particles (VLP) with properties resembling retroviruses were isolated from the liver of Wistar rats. Molecular hybridization and CRIA test were used for further analysis of the VLP. The CRIA method showed that VLP preparation lacked antigenic determinants of the major internal protein of C-type virus. By the dot hybridization technique no homology was detected between VLP and Mo-MuLV DNA, however VLP RNA was found to be homologous to IAP (interstitial A particles) DNA of mice. VLP proviral DNA was detected in the rat genome by the blot hybridization technique. Thus, it was concluded that VLP resemble IAP. A possible role of rat IAP expression is discussed.

Animals↗
PubMed1985

Reverse transcriptase of rat liver associated with the endogenous retrovirus related to the mouse intracisternal A-particles.

Reverse transcriptase activity was found in rat liver enclosed in virus-like particles. Through hybridization with DNA probes of A- and C-type retroviruses and with the help of electron microscopy the virus-like particles have been identified as endogenous retroviruses related to the mouse intracisternal A-particles. Blot hybridization revealed the provirus DNA in the rat genome. The enzyme was isolated from the virus-like particles, purified and characterized. The main properties of the enzyme resemble those of the mammalian retrovirus reverse transcriptase.

Animals↗
PubMedDate not supplied

[Changes in the RNA-dependent DNA-polymerase activity of retrovirus-like particles in the rat liver during postnatal ontogeny].

The RNA-dependent DNA-polymerase activity was studied in the postmicrosomal fraction and in the microsomal sediment of the liver of the newborn and adult Wistar rats. In the microsomal sediment of 4-6 day old rats the RNA-dependent DNA-polymerase activity was approximately by one order of magnitude higher than in that of 2 week old and adult rats. In the postmicrosomal fraction of 3 day old rats the RNA-dependent DNA-polymerase activity was also higher, but only by 30-35%, than in that of animals of the other age groups. Particles with density of 1.17 g/ml were found in the microsomal sediment of all studied animals. The characteristic morphology, sensitivity of the particle RNA-dependent DNA-polymerase activity to RNAse and the presence of reverse transcriptase allow for these particles to be referred to as retroviruses. A suggestion is put forward that the high intensity of reverse transcription during the early postnatal period can be due to still continuing processes of cell differentiation and enzymatic imprinting.

Animals↗
PubMedDate not supplied

[Relation of RNA-dependent DNA-polymerase from the rat liver with virus-like particles].

RNA-dependent DNA-polymerase activity was found in the 165 000 g supernatant and pellet of the postmitochondrial rat liver fraction. Further fractionation of the 165 000 g pellet in the linear sucrose gradient (20-50%) showed that RNA-dependent DNA-polymerase activity was distributed between fractions with densities 1.18-1.19 g/ml and 1.09-1.1 g/ml. In the fractions with 1.18-1.19 g/ml density the enzymic activity could be detected only after Triton X-100 treatment and disappeared after the incubation with pancreatic ribonuclease A. Triton X-100 treatment of the 165 000 g supernatant and the fractions with density 1.09-1.1 g/ml did not increase further the enzymic activity. Electron microscopy revealed in the 1.18 g/ml fraction virus-like particles resembling retroviruses of A and C type. In the light peak "non-mature" virus-like particles were found. The 165 000 g supernatant devoid of virus-like particles contained free RNA-dependent DNA-polymerase activity. The virus-like particles of both types seem to be endogenous rat retroviruses serving as a source of the particular and free reverse transcriptase in the rat liver.

Animals↗
PubMedDate not supplied

[Use of a synthetic oligonucleotide (CAC)5 in the genomic "dactyloscopy" method].

Potentialities of a chemically synthetized oligonucleotide of a certain structure in the genomic "dactyloscopy" method were under study. A random sample analysis has demonstrated a high resolving power of this variant of the method. Arguments in favor of introducing this approach in practical forensic medical direct identification of biologic material are presented.

DNA Fingerprinting↗