Search PubMed⌕ Search

Biomedical subjects

N Onoda

Publications and source records attributed to N Onoda.

At least 91 records · Page 5Linked to original sources
PubMed1995

[Gene expression in gastric carcinoma tissues].

In gastric cancer, the expression of c-myc protein and mRNA were investigated to determine the mechanism of inappropriate expression of c-myc gene. Correlation between clinical findings and the gene was also studied. Forty-six lesions of 43 patients were investigated by immunohistochemical staining technique. The expression of c-myc mRNA in the small fresh tissues of the same patients was detected by Northern blot hybridization. Results were as follows: 1) c-myc protein was detected in 20 lesions. The expression of c-myc protein was not differed in histological types ans stages of the lesions. 2) The level of c-myc mRNA expression was increased. In 34 cancer lesions than normal mucosa of the same patients. The overexpression of c-myc mRNA in the cancer lesion was more frequently found in early-stage cancer than in advanced-stage cancer. However, no difference was seen in histological types. 3) c-myc protein expression was correlated with mRNA overexpression. 4) In early-stage cancer, the rate of the lesions found to express excess c-myc protein was not so frequent as that of mRNA overexpression. In conclusion, c-myc mRNA overexpression was thought to cause inappropriate protein expression in gastric cancer and c-myc gene overexpression might happen already in the early-stage of gastric cancer suggesting it had a role in carcinogenesis.

Adult↗
PubMed1995

[Malignancy of gastric cancer analyzed by the expression of thymidine phosphorylase].

In this study, the expression of thymidine phophorylase (dThdPase) was investigated immunohistochemically in 73 patients with gastric cancer. dThd Pase was mainly distributed at the apical portion, or cytoplasm of cancer cells and its expression was detected in 52 tumors (71.2%). There were no significant association among the expression of dThdPase, histologic type, depth of invasion, and lymph node metastasis. However, a significant difference was noted with respect to liver metastases. Among 73 patients, liver metastases were observed in 11 patients. dThdPase expression was detected in all of these cases with liver metastases, which was significantly more common than those without such metastases. In relation to the prognosis, patients with dThdPase-positive tumors had a significantly worse prognosis than those with dThdPase-negative tumors. In conclusion, the expression of dThdPase may be useful as a predictor of malignant potential in gastric cancer.

Adult↗
PubMed1994

Signal propagation from piriform cortex to the endopiriform nucleus in vitro revealed by optical imaging.

Optical signals were recorded from the posterior piriform cortex slices of guinea pigs stained with a voltage-sensitive dye to analyse spatio-temporal spread of neural activity evoked by electrical stimulation of afferent fibers. After propagation of activity along layers II and III, an isolated island of activity appeared deep to the layer III and moved caudally. Histological inspection revealed that the area where the island appeared corresponded well to the endopiriform nucleus. The present results provided an evidence for one of the main outflows of olfactory information from the posterior piriform cortex.

Animals↗
PubMed1994

Proliferating cell nuclear antigen labeling index of preoperative biopsy specimens in gastric carcinoma with special reference to prognosis.

BACKGROUND: Proliferating cell nuclear antigen (PCNA) is an auxiliary protein of DNA polymerase delta and is considered to correlate with the cell's proliferative state. Immunohistochemical methods with a anti-PCNA antibody (PC10) have particular advantages compared with other techniques because of the maintenance of the tissue architecture and simplicity of the methodology. The authors investigated the correlation between the tumor's proliferative activity and progression of gastric carcinoma using immunohistochemical staining with PC10. METHODS: One hundred eighty-nine endoscopic biopsy specimens from patients with gastric carcinoma before operation were investigated by immunohistochemical study, using anti-PCNA monoclonal antibody (PC10). The correlation of PCNA labeling index (percentage of positive cells in more than 500 tumor cells) with various clinicopathologic factors and prognosis was studied. RESULTS: The PCNA labeling index became higher as the histologic stage increased. In patients with lymph node metastasis, vascular invasion, liver metastasis, and peritoneal metastasis, the labeling index was significantly higher. In patients with high PCNA labeling index (50% or greater), prognosis was significantly poorer than in those with a low index (less than 50%). The rate of disease recurrence after curative resection was significantly higher in patients with high PCNA grade group. CONCLUSION: As a result of this study, PCNA labeling index is suggested to be correlated with the depth of invasion, organ metastasis, vascular invasion, and tumor stage and to be effective as a prognostic factor.

Adult↗
PubMed1994

Optical imaging of the in vitro guinea pig piriform cortex activity using a voltage-sensitive dye.

The spatio-temporal patterns of signal processing in guinea pig piriform cortex (PC) slices were analyzed by optical imaging using a voltage-sensitive dye. Slices (400 microns thick) were cut in a plane parallel to the lateral olfactory tract and perpendicular to the cortical surface. In all the anterior PC and the majority of the posterior PC preparations, neural activity elicited by electrical stimulation of layer Ia propagated along the same layer, then it invaded into layers II and III and propagated along them. In addition to the above pattern, invasion of activity into the deeper area than layer III was observed in some posterior PC preparations. Real-time imaging of an active zone evoked by Ia shocks and its spatio-temporal behavior will contribute to resolving olfactory information processing.

6-Cyano-7-nitroquinoxaline-2,3-dione↗
PubMed1994

Characterization of thyroid hormone effect on the visual system of the adult rat.

To characterize the effect of thyroid hormone on the central nervous system in adult rats, we recorded evoked potentials by photic (VEP) and electrical stimulation in normal, thyroidectomized (Tx) and T3- or T4-supplemented (Tx-T3, Tx-T4) male adult rats under pentobarbital anesthesia. The latencies of VEPs recorded in the visual cortex (VC) and lateral geniculate nucleus (LGN), and the b-waves of the electroretinograms (ERG) of these three groups were compared. A significant prolongation of the latency of VEP by photic stimulation (flash light intensity 2J, 0.2 Hz) in the VC was observed 14 to 28 days after thyroidectomy (27.9 +/- 0.4 vs. 33.4 +/- 0.43 msec, p < 0.01). Similar prolongations were also observed when those of the VEP in the LGN and the b-wave of the ERG were compared to those of normal rats (22.4 +/- 0.37 vs. 27.3 +/- 0.41 msec, p < 0.01; 21.8 +/- 0.21 vs. 25.3 +/- 0.41 msec, p < 0.01, respectively). The prolonged latencies observed in the VC, LGN, and ERG-b-wave were partially normalized 24 hr after T3 supplement (50 micrograms/kg, sc) and fairly restored to normal levels by 48 hr after T4 injection (100 micrograms/kg sc). The VEP latencies in the LGN and VC showed a positive correlation with the b-wave latency of the ERG in these three groups.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1994

[Establishment and characterization of a human breast cancer cell line, OCUB-1].

We established new human breast cancer cell line, OCUB-1 derived from the pleural effusion of 53-year old female with recurrent breast cancer. Two sub-cell lines were also established with cloning technique from OCUB-1. Investigating the differences of characteristics between OCUB-1M and 1F, OCUB-1M shows a monolayered growing and OCUB-1F grows in floating. OCUB-1M and 1F revealed Modo 84 and 41 by chromosomal analysis and flow cytometric analysis showed OCUB-1M had twice amount of DNAs as -1F. OCUB-1M revealed higher expression of E-cadherin and laminin receptor against-1F, and activity of 92kDa type IV collagenase could be seen only in OCUB-1M. Estorogen and progesteron receptor were negative in either OCUB-1M and -1F, and no production of tumor markers in the spent media (CEA, CA19-9, CA15-3 and NCC-ST439) was detected in both cells. Both cells could be hetero-transplanted in nude mice, but they showed different histology.

Animals↗
PubMed1994

[Nuclear DNA ploidy pattern and proliferating cell nuclear antigen labeling index in gastric cancer with mucosal involvement].

The DNA ploidy pattern and the expression of proliferating cell nuclear antigen (PCNA) were investigated in 72 gastric carcinoma patients to determine the possible correlation between proliferation kinetics and biological behavior. The diploid pattern was more often observed in cases with mucosal invasion (m cancer) than other cases (sm or advanced cancer). The PCNA labelling index in cases with mucosal invasion was significantly lower than in other cases. The sm cancer was more often observed in patients with high PCNA labelling index (> or = 30%) and aneuploid pattern, while m cancer was more common in patients with low PCNA labelling (< 20%) index and diploid pattern. As a result of this study, we conclude that proliferating kinetics of m cancer may differ from that for sm or advanced cancer. Furthermore, DNA ploidy pattern and PCNA labelling index may be useful as a parameter of depth of invasion.

Adult↗
PubMed1993

Inhibition of human pancreatic cancer cell (MIA PaCa-2) growth by cholera toxin and 8-chloro-cAMP in vitro.

The effects of cholera toxin (CT) and 8-chloro-cAMP (8-Cl-cAMP) on cell growth were investigated using two human pancreatic carcinoma cell lines (MIA PaCa-2, Panc-1). CT, which catalyses the ADP ribosylation of Gs, suppresses the proliferation of MIA PaCa-2(PC) cells. CT at the low dose of 0.1 pg ml-1 was inhibitory of PC cell growth, and the maximum suppression (70%) was achieved at a CT concentration of 100 pg ml-1. This phenomenon was reversible. The production of cAMP by CT (100 pg ml-1) in PC cells was enhanced 320-fold compared with the control. In addition, cAMP analogues (8-Cl-cAMP, 8-Br-cAMP) and forskolin decreased the growth rate of PC cells in a dose-dependent manner. These results support the view that CT suppresses PC cell growth by stimulating cAMP production. Conversely, Panc-1 cells were far less sensitive to CT in cell growth and cAMP production. 8-Cl-cAMP was also less effective on Panc-1 cell growth. The binding of an insulin-like growth factor (IGF)-I and transforming growth factor (TGF)-alpha, which has been shown to stimulate PC cell growth in an autocrine manner, to PC cells was not modified in cells treated with CT or 8-Cl-cAMP. The results suggest that the inhibitory actions of these substances do not occur at the level of the receptor for IGF-I or EGF/TGF-alpha. We have previously shown that phorbol esters, which decrease the binding of TGF-alpha to PC cells, has an anti-proliferative activity on these tumour cells. Inhibited cell growth by maximum suppressive dose of CT or 8-Cl-cAMP was further inhibited by TPA. In addition, an oncogene product of K-ras which is commonly activated in pancreatic cancer, was increased by CT and 8-Cl-cAMP. It is concluded that CT and 8-Cl-cAMP inhibit PC cell growth, presumably in a similar manner, and their mechanism(s) of action may be different from that of TPA. The anti-proliferative effect of CT or 8-Cl-cAMP was enhanced by TPA, implying that the combination of these substances results in increased inhibition of the PC cell growth.

8-Bromo Cyclic Adenosine Monophosphate↗
PubMed1993

Early cancer of the gastric remnant with special reference to the importance of follow-up of gastrectomized patients.

Seven patients with early cancer of the gastric remnant (group 1) and nine with gastric remnant cancer that developed after surgery for early gastric cancer (group 2) were studied clinicopathologically. In group 1, the great majority of cases were classified as type I by gross type. Most lesions existed in the posterior wall of the remnant stomach. All lesions were differentiated carcinomas. In group 2, six of the nine early gastric tumors resected at the initial surgery were present in site A and three in site M. Gross tumours were of various types. Six patients underwent Billroth II resection and two underwent Billroth I resection. At second surgery (for gastric remnant cancer) four lesions were found to be located in the gastric stump or anastomosed region and five in the posterior wall of the stomach remnant. Histologically, the lesions included four undifferentiated carcinomas and five differentiated carcinomas. Of the nine lesions involving the gastric remnant, only two were early stage: the remaining seven were in an advanced stage. Prognosis was extremely poor in the group, many patients dying of carcinomatous peritonitis. In summary, most group 1 patients had a type I lesion in the remnant stomach. In group 2, more than half of the lesions were present in the gastric stump or anastomosed site and most were in an advanced stage. These observations suggest that it is very important to follow up patients operated on for early gastric cancer in order to detect malignant lesions, if any, in the remnant stomach before disease progresses to a stage at which radical resection is no longer possible. Follow-up will no doubt improve long-term results following surgery for early gastric cancer. We consider endoscopic examination and biopsy, especially of the gastric stump and anastomosed site, of great importance for gastrectomized patients.

Aged↗
PubMed1993

[Analysis of cell proliferation in endoscopically biopsied specimen of the stomal mucosa of the remnant stomach--with special reference to cell cycle and ODC activity].

An attempt was made to investigate the status of cell proliferation in the remnant stomach, using endoscopically biopsied specimens, with cell cycle analysis by flow cytometry (FCM) and ornithine decarboxylase (ODC) activity determination. Fifty-three partially gastrectomized patients (male 33, female 20) were examined. Their mean age was 60.5, and 22 underwent Billroth I (BI), and 30 Billroth II (B II) operation. The mean time elapsing from the initial operation was 4.0 years. Almost all specimens showed DNA diploidy by FCM. The S phase cell percentage was higher in the stomal area, especially in the B II group. Significantly higher ODC activity was recognized in the stomal mucosa especially in the B II group. A significant positive relationship was noted between postoperative duration and ODC activity in the B II group. These results suggest activated cell proliferation in the stomal area of the remnant stomach. Especially in the B II group, cell proliferation increased with time after initial operation. This activated cell proliferation may play some role in the carcinogenesis of the remnant stomach.

Biopsy↗
PubMed1992

Odor-induced fos-like immunoreactivity in the rat olfactory bulb.

We here report odor-induced mapping patterns of c-fos-like protein (Fos) immunoreactivity in the rat olfactory bulb under urethane anesthesia. Regional patterns of cells with nuclei expressing Fos, plotted on a spread-out reconstruction of the mitral cell layer, were strikingly comparable to data from the 2-deoxyglucose method, by which different foci of the most-labelled glomeruli of metabolic activity were demonstrated using different odors. Cells in the glomerular, external plexiform, and granule cell layers were also labelled.

Animals↗
PubMed1992

Thyroid ultrasonography related to clinical and laboratory findings in patients with silent thyroiditis.

We summarized the clinical course of 10 patients with silent thyroiditis and evaluated the clinical usefulness of ultrasonography, in combination with clinical and laboratory findings, for the differentiation from Graves' disease. Serum T3 and T4 were increased in all cases, and the ratio of T3/T4 (ng/micrograms) was 17.8 +/- 3.6 (SD). But in 3 of 10 patients the ratio was greater that 20. TSH receptor antibody (TRAb) and thyroid stimulating antibody (TSAb) were negative in all cases. The estimated thyroid volume by ultrasonography was 18.4 +/- 5.5 ml, which was slightly increased but significantly lower than those in Graves' disease (p less than 0.05). The internal texture of the thyroid showed a decreased echogenicity with a mean echo level of 70.4 +/- 15.4. There was a weak positive correlation between the echo level at the onset of thyrotoxicosis and the lowest T3 level during the clinical course (p less than 0.05). It is suggested that ultrasonography gives a useful information to the diagnosis and outcome of patients with silent thyroiditis.

Adult↗
PubMed1992

Monoclonal antibody immunohistochemistry of a temporal relationship between axonal elimination of aberrant olfactory nerves and synaptogenesis in the rabbit olfactory bulb during middle embryonic periods.

Immunostaining using olfactory nerve- and synaptic vesicle protein-specific monoclonal antibodies revealed their characteristic appearance in the rabbit olfactory bulb during prenatal development. Prior to formation of glomeruli, olfactory nerve fibers extended beyond their target region deep into the bulb zones. Subsequently the aberrant axons decreased in number, and correspondingly, synaptic vesicle protein occurred in the innermost region of the olfactory nerve layer. It is concluded that the lack of synaptogenesis causes axonal elimination of aberrant olfactory nerves. Present results support the hypothesis that supernumerary axons degenerate unless synaptic contacts are secured; the olfactory nerves must arrive at the appropriate terminal zone in the glomeruli.

Animals↗