Search PubMed⌕ Search

Biomedical subjects

N Ohta

Publications and source records attributed to N Ohta.

At least 307 records · Page 17Linked to original sources
PubMed1983

[Clinical application of a new right subcostal approach in the evaluation of interatrial septal motion].

We developed a new echocardiographic approach to detect the interatrial septum (IAS) by tilting a transducer leftward, cephalad and backward from the right subcostal area. This technique could allow us to visualize the IAS moving perpendicularly to the ultrasonic beam. In normal subjects the IAS showed a small posterior deflection moving toward the left atrium due to atrial contraction following the P wave of the electrocardiogram. Following the onset of ventricular ejection the IAS rapidly moved anteriorly. During diastole the IAS showed an initial rapid posterior displacement and then a more gradual slope, reflecting rapid and slow filling phases, respectively. The magnitude and configuration of IAS motion showed variations dependent on atrial conditions of cardiac diseases. In atrial fibrillation there were f waves on the IAS echogram and in mitral stenosis a septal notch was recorded at the timing of a mitral opening snap. In mitral valve prolapse there was also a midsystolic notch of the IAS echogram almost coincident in time with the onset of a late systolic murmur. On the other hand, the IAS revealed a systolic increased excursion or paradoxical motion in mitral or tricuspid regurgitation, respectively. In hypertension and myocardial infarction the atrial kick of the IAS echogram showed an exaggerated excursion. In 125 out of the consecutive 150 cases (83.3%) we could record satisfactory IAS echograms by this new approach.

Adolescent↗
PubMed1982

Association between HLA and Japanese patients with rheumatoid arthritis.

Japanese patients with rheumatoid arthritis (RA) were observed to have a statistical association with HLA-DR4, MT3. Strong association between the clinical severity of RA and HLA was also observed. Male patients had a stronger association with HLA than female patients. Males are more resistant to RA than females. This suggested that the threshold of liability for RA is higher in males than in females. Japanese patients with RA with systemic vasculitis were negative for HLA-Bw44 and had antilymphocytotoxic autoantibody, indicating that RA with systemic vasculitis is different in etiology from RA without systemic vasculitis.

Adult↗
PubMed1982

Isolation and expression of cloned hook protein gene from Caulobacter crescentus.

Previous genetic analysis of Caulobacter crescentus showed that the periodic synthesis of hook protein, flagellin A, and flagellin B, the major flagellar subunits, is coupled in some way to chromosome replication. To examine the regulation of flagellar gene expression at the molecular level, we isolated the gene that codes for the 72,000-dalton hook protein. A specific 125I-labeled anti-hook protein IgG was used to screen a hybrid lambdaL47.1 bank of 4,500 clones and to compare peptide maps of the cloned gene product with purified hook protein. Restriction analysis of DNA from the positive lambda clones and plasmid subclones showed that the structural gene for the hook protein is contained on a 2.3-kilobase (kb) BamHI fragment. The direction of transcription was established by demonstrating the inducibility of hook protein gene in strains with the 2.3-kb fragment fused to the Escherichia coli lipoprotein gene-lactose gene promoter-operator region of pIN-II. Preliminary genomic analysis showed that the hook gene occupies a single location on the C. crescentus chromosome. These results suggest that the periodic expression of the hook protein gene in the cell cycle does not involve a major or persistent rearrangement of the 2.3-kb coding sequence during the cell cycle.

Bacteria↗
PubMed1982

HLA and clinical manifestations in Takayasu disease.

In previous studies on HLA antigens in patients with Takayasu disease, we found a statistically high frequency of haplotype Bw52-Dw12. Clinico-pathological conditions of this disease observed for 85 +/- 3 months were compared between 29 patients with (positive group) and 39 patients without this haplotype (negative group), among 82 patients with Takayasu disease. Blood sedimentation rate and C-reactive protein exhibited statistically significant high figures in the positive group as compared with those in negative group, while no difference were seen in rheumatoid arthritis test, antistreptolysin-0 titer, anti-DNA antibody, levels of circulating immune complexes, platelet aggregation, although all revealed high levels in the positive group. Pulmonary disorders were more frequent in positive group. Fourteen patients (48%) in the positive group and 5 (13%) in the negative group had abnormalities in the aortic valve (p less than 0.01). In 14 patients in the positive (38%) and 5 in the negative (13%) groups, the systolic blood pressure was over 140 mmHg (p less than 0.01). Four patients in the positive (17%) and one in the negative groups (3%) were blind (p less than 0.01). These data suggest that in patients with a haplotype of Bw52-Dw12, there is a greater likelihood of an active inflammatory state and a rapid progression of these morbid conditions.

ABO Blood-Group System↗
PubMed1982

Immunogenetic analysis of patients with post-schistosomal liver cirrhosis in man.

Immune responsiveness of 121 patients with post-schistosomal liver cirrhosis to schistosomal antigens was investigated. Out of 78 patients, only five (6.4%) showed low responsiveness to schistosomal adult worm antigen whereas 73 (93.6%) were high responders. Out of 57 healthy individuals with previous schistosomal infection, low responders were found in 17.5%. The frequency of low responders to schistosomal adult worm antigen was significantly decreased in the patients with post-schistosomal liver cirrhosis (P less than 0.05). Out of 121 patients, a significant increase in frequency of HLA-Bw44-DEn haplotype was observed (corrected P less than 0.02). On the other hand, HLA-Bw52-Dw12 haplotype which was reported to be in strong linkage disequilibrium with an immune suppression gene for schistosomal adult worm antigen was significantly decreased (corrected P less than 0.005). These observations suggested that an HLA-linked immune suppression gene controlled susceptibility or resistance to post-schistosomal liver cirrhosis through regulation of immune responsiveness of the hosts to schistosomal antigen in man.

Adult↗
PubMed1982

[Experience with cefmetazole in the urological fields].

Recently, a new antibiotic of cephamycins, cefmetazole (CMZ) has been developed. In our clinic, CMZ was used to examine its clinical effect and adverse reactions, and the results were herein reported. The CMZ was administered to 8 patients for the prevention of postoperative infections, 7 with genitourinary infections and 1 with maxillitis complicated with uremia. For these patients, a daily dose of 1 to 6 g of CMZ was intravenously given for a period of 4 to 12 days. Among the 8 patients who received CMZ for the prevention of postoperative infections, there were 1 case with urinary tract infection, 1 with wound infection and 2 with fever of over 38 degrees C, but they had no serious infections. In the clinical observations on 3 cases with pyelonephritis, the CMZ showed marked effectiveness in 2 cases and effectiveness in 1 case. It must be noted that CMZ was proved to be markedly effective for pyelonephritis caused by Serratia marcescens. In the cases with acute epididymitis and prostatitis, a good effect of CMZ was obtained. Similarly in the case with maxillitis complicated with uremia, the maxillitis was improved by the effect of CMZ and hemodialysis. No cases with hepatic or renal dysfunction were observed after using CMZ. It is concluded that satisfactory effect of CMZ was obtained clinically.

Adolescent↗
PubMed1980

Association between an HLA haplotype and low responsiveness to schistosomal worm antigen in man.

Immune responsiveness to schistosomal worm antigen was investigated in the individuals infected with Schistosoma japonicum by measuring the antigen-specific proliferative response of the peripheral T lymphocytes in vitro. Out of 57 infected individuals, 10 (17.5%) were low responders, whereas 47 (82.5%) were high responders to this antigen. The strong association between the HLA-Aw24-Bw52-Dw12 haplotype and the low responder group was demonstrated. Because the association of the low responder group was strongest with the HLA-D specificity, Dw12, and because the HLA-D region was assumed to be comparable to the I region of the murine H-2 complex, it was suggested that the low responsiveness to schistosomal worm antigen was controlled by a single dominant immune suppression gene that was in strong linkage disequilibrium with HLA-Dw12.

Antigens↗