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Biomedical subjects

N Ohhata

Publications and source records attributed to N Ohhata.

10 recordsLinked to original sources
PubMed1994

WS79089A, B and C, new endothelin converting enzyme inhibitors isolated from Streptosporangium roseum. No. 79089. Taxonomy, fermentation, isolation, physico-chemical properties and biological activities.

WS79089A, B and C, which are novel endothelin converting enzyme (ECE) inhibitors have been isolated from the fermentation broth of Streptosporangium roseum No. 79089. These inhibitors were purified from an acetone extract of whole culture broth followed by Silicar CC-4 column chromatography and HPLC. WS79089A, B and C showed highly selective ECE inhibition activity with IC50 values of 0.73 microM 0.14 microM and 3.42 microM, respectively. On the basis of spectroscopic and chemical evidence, the tentative structures of WS79089A, B and C have been proposed, they have benzo[a]naphtacen chromophores.

Actinomycetales↗
PubMed1992

WS009 A and B, new endothelin receptor antagonists isolated from Streptomyces sp. no. 89009. I. Taxonomy, fermentation, isolation, physico-chemical properties and biological activities.

WS009 A and B novel endothelin receptor antagonists, have been isolated from the fermentation broth of Streptomyces sp. No. 89009. These antagonists were purified from the culture filtrate followed by Diaion SP-207, DEAE Toyopearl column chromatography and HPLC. WS009 A and B showed selective activity in an endothelin receptor binding assay with IC50 of 5.8 x 10(-6) M and 6.7 x 10(-7) M, respectively. On the basis of spectroscopic and chemical evidence, the structures of WS009 A and B have been established as 1 and 3, and are highly hydroxylated benz[a]anthraquinone chromophores.

Anthraquinones↗
PubMed1990

[Three-dimensional morphological analysis for craniofacial deformity].

Morphological analysis of the craniofacial deformity has been performed by cephalometric radiography, but even with this method, it is difficult to analyze the cases with complicated deformity. The purpose of the present study is to develop the new 3-D CT morphological analyzing system in order to investigate complicated craniofacial deformity. Using the 67 landmarks, we established the 3 D line drawing method. Then the reproducibility of the landmarks were examined and the clinical cases were investigated. The measurement of landmarks were carried out 10 times by the same operator, and standard deviation of 67 landmarks were calculated. Some landmarks which were difficult to identify showed high value of standard deviation. However, it was almost same value between standard deviation of most of the landmarks and the CT system error. Therefore, location of landmarks in the system were found to be reproducible. This new analyzing system was performed in clinical cases with craniofacial deformity. In such cases, cranium deformity and complicated deformity such as twist-form arrangement of cranium, maxilla and mandible were detected. The 3-D CT morphological analysis is effective for analyzing severe craniofacial deformity which was impossible to recognize with conventional cephalometric analysis.

Cephalometry↗
PubMed1976

The distribution of the third complement component and other polymorphic traits in rheumatoid arthritis.

The distribution of C'3 phenotypes in 61 seropositive rheumatoid arthritis patients and 464 normal blood donors in Tokyo was examined by high-voltage agarose gel electrophoresis. The allele of the common European variant, C3(F), is absent in Japanese, but another F allels, C3(Frare), is present with a frequency of 0.025 and 0.013 in RA patients and blood donors, respectively; the difference is not statistically significant. The distribution of phenotypes for several other enzyme systems was also determined in an attempt to detect association with RA Again, no statistically significant differences were found.

Arthritis, Rheumatoid↗
PubMedDate not supplied

Three-dimensional analysis of craniofacial bones using three-dimensional computer tomography.

We studied the three-dimensional (3D) deformities in patients with congenital facial anomalies, such as cleft lip and palate or hemifacial microsomia using three-dimensional CT (3DCT) images. At the same time, the data from the 3DCT was processed and analyzed using a 3DCT measurement system which we have recently developed. The coordinates of the 67 test points on the craniofacial bones were determined on a 3D image prepared by the 3DCT measurement system. Using this system, we prepared the 'skeletograms', which present deformities of craniofacial bones in detail as a 3D wire frame model, for detailed analysis of the craniofacial bones' deformities.

Adolescent↗