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Biomedical subjects

N Navarro

Publications and source records attributed to N Navarro.

35 records · Page 2Linked to original sources
PubMed1988

Human melanoma cell lines: an immunofluorescence and ultrastructural study.

Two human melanoma cell lines (OAL-1 and OAL-2) were obtained from primary tumors. Electron microscopy and indirect immunofluorescence for S-100 protein and human melanoma associated antigen p97a were used to characterize the cell lines. Ultrastructural correlation between primary tumor cells and cultured cells was investigated. The ultrastructural features of OAL-2 cells were similar to those of primary tumor cells. However, OAL-1 cells showed a fibroblast-like appearance, quite different from that of the primary tumor cells. This fibroblast-like appearance persisted after 50 subcultures of the OAL-2 cell line and suggests cellular dedifferentiation. No spontaneous redifferentiation of the cells to the original phenotype of the primary tumor cells was observed even after 50 subcultures.

Aged↗
PubMed1988

An ultrastructural analysis of vascular leiomyoma of the bladder.

We report a case of vascular leiomyoma of the bladder in a 62-year-old man. To our knowledge, this is the first ultrastructural study published on vascular leiomyoma of the bladder. The tumoral cells reproduced the smooth muscle cells. The vascular structures corresponded to capillaries and arterioles. We consider that the vascular component is not tumoral and its origin is related to mast cells.

Arterioles↗
PubMed1987

Metastatic granulosa cell tumor with pattern of sex cord tumor with annular tubules.

A case of malignant, complex sex cord tumor with multiple differentiations in a 38-year-old woman is presented. The neoplasm presented originally as a pure granulosa cell tumor, and in the second look operation as a sex cord tumor with annular tubules (SCTAT). Six years later abdominal metastases revealed a mixed pattern of granulosa and Sertoli cell tumor admixed with SCTAT. The patient died despite chemotherapy. Analysis of the 10 previously reported cases of malignant SCTAT shows that tumor size is the main prognostic indicator. Mitoses, atypia, and stromal invasion seem also to bear prognostic implications when present. The association of both granulosa and Sertoli cells with SCTAT seems to provide a clue for SCTAT being a peculiar intermediate phenotypical expression of pluripotential stem sex cord cells of the gonads.

Adult↗
PubMed1987

Uterine intravascular leiomyomatosis: an update and report of seven cases.

A clinicopathological study of seven cases of intravascular leiomyomatosis (IVL) was performed. The patients' ages ranged from 29 to 80 and all cases were associated with uterine leiomyomas. The intravascular extension involved preferentially uterine vessels, although the adnexa were also affected. Microscopically, the intravascular tumors resembled the primary leiomyomas. Mitotic count was low and never exceeded 3 per 10 high-power fields. Thick venous vessels, sometimes with thrombosis, were a conspicuous marker in intravascular locations. Necrosis and hemorrhage were found in four cases. Epithelioid IVL was found in one instance associated with a lipid cell tumor of the ovary. This series supports, for most cases, the notion of an origin from a preexisting leiomyoma. A review of treatment, prognosis, and histogenesis of IVL is performed.

Adnexa Uteri↗
PubMed1984

Effects of several alimentary fats on serum lipids during long-term stabilized diets.

The intake of a stable diet during 25 months by 68 women living in a closed religious community allowed us to study the effects of various alimentary fats on the serum lipids. Thus we could observe the consequences of the quantitatively and qualitatively different fatty acids in the diet, while the total quantity of lipids and the other constituents of the dietary intake were unaltered. The serum total cholesterol and apoprotein B levels are correlated with the P/S ratio of the diet; on the contrary, the serum HDL cholesterol and apoprotein A levels are not correlated with the fatty acid composition of the diet. The moderate supply in lipids (30% of the total caloric intake) and cholesterol (300 mg/day) maintained during this study corresponds to the usual dietary recommendations. Therefore, not very high levels of serum total cholesterol were observed, whichever vegetable oil was consumed.

Adult↗
PubMed1981

Mucosal secretin content in postmortem tissues.

Since human tissues are generally obtained at autopsy performed some hours after death, it is necessary to extrapolate from the observed concentration what the values might have been antemortem. In this study, we investigated the changes of secretin in gastrointestinal mucosa in man and rats under differing conditions postmortem. Although considerable secretin can be recovered from mucosal tissue even after extended postmortem delay, these studies demonstrate the importance of low postmortem body temperature and rapid removal and extraction of tissues after opening of the peritoneal cavity to maximize recovery.

Animals↗
PubMed1978

Comparative effects of canbra oil and butter on lipdemia, vitamin A tolerance and thrombosis factors in man.

In a five-day clinical test, the dietary effects of canbra oil and butter on normal human subjects were studied with special reference to lipid metabolism, clotting times and platelet functions. There was no difference between the canbra oil and butter diets as regard triglyceridemia or clotting times, but cholesterolemia and in vitro platelet aggregation by ADP dropped significantly in subjects fed the canbra oil diet. The platelet count did not change significantly. A possible explanation of these results might to be the difference in the fatty acid composition of canbra oil and butter.

Adolescent↗
PubMedDate not supplied

Rapid determination of alpha emitters using Actinide resin.

The European Commission has recently published the recommended radiological protection criteria for the clearance of building and building rubble from the dismantling of nuclear installations. Radionuclide specific clearance levels for actinides are very low (between 0.1 and 1 Bq g(-1)). The prevalence of natural radionuclides in rubble materials makes the verification of these levels by direct alpha counting impossible. The capability of Actinide resin (Eichrom Industries, Inc.) for extracting plutonium and americium from rubble samples has been tested in this work. Besides a strong affinity for actinides in the tri, tetra and hexavalent oxidation states, this extraction chromatographic resin presents an easy recovery of absorbed radionuclides. The retention capability was evaluated on rubble samples spiked with certified radionuclide standards (239Pu and 241Am). Samples were leached with nitric acid, passed through a chromatographic column containing the resin and the elution fraction was measured by LSC. Actinide retention varies from 60% to 80%. Based on these results, a rapid method for the verification of clearance levels for actinides in rubble samples is proposed.

Journal Article↗
PubMedDate not supplied

Method for actinides and Sr-90 determination in urine samples.

The primary goal of radiation protection in decommissioning and decontamination of the old nuclear facilities of the CIEMAT is to monitor and minimize exposure of personnel. Monitoring programs include determination of actinides and 90Sr in biological samples. A technique for the sequential measurement of low levels of 239Pu, 241Am and 90Sr in urine samples has been developed. The method involves coprecipitation of these radionuclides as phosphates from bulk urine sample. Separation of Plutonium is carried out using a conventional anion exchange technique. Americium and strontium isolations are achieved sequentially by chromatographic extraction (Tru.Spec and Sr.Spec columns) from the load and rinse solutions coming from the anion exchange column. Plutonium and Americium measurements are performed by alpha spectrometry. The mean recovery obtained is 80% and the detection limit for 24 h urine sample (1.41) is 0.6 mBq L-1. 90Sr determination is made by liquid scintillation counting. The detection limit in this case is 1.1 E-01 Bq/L.

Actinoid Series Elements↗