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Biomedical subjects

N N Kozlov

Publications and source records attributed to N N Kozlov.

13 recordsLinked to original sources
PubMed2003

First RFLP linkage map of red clover ( Trifolium pratense L.) based on cDNA probes and its transferability to other red clover germplasm.

We constructed a genetic linkage map of red clover ( Trifolium pratense L., 2n=2 x=14) using RFLP markers from cDNA probes of a backcrossed mapping population, and investigated the transferability of the markers to other red clover germplasm. The map contains 157 RFLP markers and one morphological marker on seven linkage groups. The total map distance was 535.7 cM and the average distance between two markers was 3.4 cM. All of the cDNA probes of the map were hybridized to the fragments of genomic DNA from 12 plants derived from three varieties, and 87% of the cDNA probes detected polymorphic bands that corresponded to those of mapping parents. This result indicated that RFLP markers on the present map were transferable to the genome analysis of other red clover germplasm. This is the first report to construct a linkage map of Trifolium species; it should provide fundamental and useful genetic information relevant to the breeding of red clover and genus Trifolium.

Chromosome Mapping↗
PubMed1993

Computer simulation of tRNA secondary structure folding.

Computer simulation results of folding linear RNA molecules into secondary structures are presented. The structure is formed by two interacting processes: the RNA molecular chain growth (beginning from an initial length, L0), and the structuring (secondary structure sequential growth in the region of the existing molecular chain, based on the local free energy minimization by sequential addition of elementary substructures--stems). It was found that the final secondary structure formation is greatly influenced by the 'structuring period' T (the ratio of the molecular chain growth rate to the structuring rate), and the direction of RNA synthesis. The computer simulation has been performed for 219 and 906 tRNA genes from two published catalogues, on the whole two-dimensional domain (T,L0) parameters, by using four known free-energy models. Minimum stem length and molecular chain growth direction have been also varied. The calculated secondary structures have been compared to the natural tRNA structures given in the catalogues, and the region of best coincidence for the model parameters has been determined. It has been proved that, on average, > 86% of the paired bases of natural tRNA structures appear in the folding simulation.

Computer Simulation↗