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Biomedical subjects

N Morlet

Publications and source records attributed to N Morlet.

At least 37 records · Page 2Linked to original sources
PubMed1997

Outcome of acanthamoeba keratitis treated with polyhexamethyl biguanide and propamidine.

OBJECTIVE: This study investigates the clinical outcome of Acanthamoeba keratitis treated with polyhexamethyl biguanide (PHMB) and propamidine isethionate (Brolene). DESIGN: A retrospective review of all patients treated for Acanthamoeba keratitis between September 1992 and February 1995 was carried out. All patients were treated with PHMB 0.02% and propamidine 0.1% hourly for 3 days, the frequency reduced to four to six times daily according to clinical response. MAIN OUTCOME MEASURES: Age, gender, result of laboratory investigation, duration of disease before diagnosis, visual acuity (VA) pretreatment and post-treatment, need for keratoplasty, and presence of adverse reaction were measured. RESULTS: One hundred eleven cases were identified in 105 patients (60 male, 45 female; mean age, 32). Ninety-two percent of infections were in contact lens wearers. The clinical diagnosis was confirmed by corneal culture or histopathology in 64 cases (57.7%). The diagnosis was made "early" (within 28 days) in 65 cases (58.6%). Twenty-one (18.9%) were "intermediate" (28 days-2 months) and 20 (18%) were "late" (> 2 months) diagnoses. Overall post-treatment VA was 6/12 or better in the majority (88/111, 79.3%) of cases, and 18 (16.2%) had VA of 6/36 or worse. The VA of > or = 6/12 was achieved by 90.8% of the early, 71.4% of the intermediate, and 65% of the late groups. Clinical relapses occurred in 19 patients on reducing the therapy. Treatment toxicity was never serious and consisted only of stinging or superficial punctate keratopathy. Keratoplasty was indicated in only ten patients, and disease activity was controlled adequately in all patients before grafting. CONCLUSIONS: Combined treatment with PHMB and propamidine is well tolerated, nontoxic, and effective. Typically, visual outcome is favorable and the requirement for keratoplasty reduced markedly.

Acanthamoeba↗
PubMed1997

A method of drop administration.

Self-administering drops can be a daunting task for many patients. The authors describe a method of drop administration that allows a patient to accurately place drops without the need for special devices or touching the eye. With the bottle of drops held upside down, the back of the thumb is rested on the forehead to stabilize the bottle. The bottle tip can be seen over the eye. The other hand retracts the inferior lid to catch the drop. Poor compliance is common and is often caused by difficulties with drop administration. This simple technique may improve compliance.

Humans↗
PubMed1996

Topical interferon alpha 2b for corneal haze after excimer laser photorefractive keratectomy. The Melbourne Excimer Laser Group.

PURPOSE: To determine whether topical interferon alpha 2b (IFN-alpha) prevents corneal haze after excimer laser photorefractive keratectomy (PRK). SETTING: Tertiary referral ophthalmic hospital. METHOD: A prospective, double-blind, placebo-controlled, randomized study of 31 patients was undertaken. After surgery in a single institution, patients received a drop of either a placebo or IFN-alpha (5 x 10(6) IU/ml) four times daily for 4 weeks. The main outcome measures were corneal haze, refraction, and visual acuity. RESULTS: The major side effect of interferon alpha treatment was a significant delay in epithelial healing by a mean of 2 days. The means of the average post-treatment clinical scores for haze in all patients up to 12 months after surgery were 0.46 +/- 0.25 for the IFN-alpha group and 0.64 +/- 0.43 for the placebo group (P = .20). Of patients with a correction of greater than 5.00 diopters (D), the IFN-alpha group had significantly less haze over the course of the study (0.39 +/- 0.23 versus 0.98 +/- 0.50; P = .03). After 12 months, the mean absolute spherical equivalent in the two groups was not significantly different (1.02 +/- 1.13 D versus 1.44 +/- 2.64 D). There was a tendency toward better uncorrected visual acuity in the INF-alpha group (P < .10, Kolmogorov-Smirnov). CONCLUSION: Topical IFN-alpha may merit further investigation as a treatment to reduce corneal haze after excimer laser PRK for corrections greater than 5.00 D.

Administration, Topical↗
PubMed1996

High dose intravitreal ganciclovir injection provides a prolonged therapeutic intraocular concentration.

BACKGROUND: Although intravitreal high dose ganciclovir has previously been found to provide excellent control of cytomegalovirus (CMV) retinitis, little was known about the vitreous concentrations of ganciclovir after a 2 mg intravitreal injection. METHODS: Eleven vitreous samples were taken from seven patients with CMV retinitis at 24 and 72 hours after a 2 mg intravitreal injection of ganciclovir and the concentration of ganciclovir was measured by high performance liquid chromatography. RESULTS: The mean concentration of ganciclovir at 24 hours was 143.8 mg/l (95% confidence interval 97.8-190) and at 72 hours was 23.4 mg/l (95% CI 0-49.7). The half life ranged from 11.9 to 26.3 (mean 18.8) hours in the four patients who had two samples taken. The mean half life calculated from the mean concentrations at 24 and 72 hours was 18.3 hours, so the calculated mean concentration at 7 days was 0.6 mg/l. CONCLUSIONS: This suggests that it takes about 7 days to eliminate the intravitreal ganciclovir, and that it is not likely to accumulate with weekly injections. The intravitreal concentrations achieved with high dose therapy remained above the ID50 for CMV (0.25-1.22 mg/l) for up to 7 days.

Antiviral Agents↗
PubMed1995

Intraoperative semiquantitative keratometry using the keratoscopic astigmatic ruler.

Intraoperative keratometry enables the surgeon to set an appropriate amount of corneal astigmatism with the suture tension. Errors in estimating or measuring the astigmatism can occur with hand-held keratometers because the distance an object is held from an astigmatic cornea influences the shape of the elliptical image. Thus hand-held instruments should be held at a calibrated distance. We have designed a new hand-held keratometer, the keratoscopic astigmatic ruler, to assess the magnitude of corneal astigmatism. It consists of a rack of elliptical keratoscopic lenses corresponding to increasing magnitudes of astigmatism and is made from inexpensive autoclavable injection-molded plastic. The ruler's keratoscopic lenses refract light, creating a real aerial image above the cornea that produces a bright and easily determined corneal reflection. The ruler incorporates a sight to allow the user to hold the ruler at the correct distance.

Astigmatism↗
PubMed1995

High dose intravitreal ganciclovir for CMV retinitis: a shelf life and cost comparison study.

BACKGROUND: It was previously found that high dose intravitreal ganciclovir provided superior treatment of cytomegalovirus retinitis compared with intravenous treatment. This study examined the stability and solubility of the ganciclovir solution to determine the shelf life of prepared solution, and compared the cost of intravitreal with intravenous therapy. METHODS: For the solubility studies high performance liquid chromatography was used to determine the ganciclovir concentration in various solutions. Measurements were taken of freshly made 20 mg/ml solution, the same solution stored at room temperature or frozen for 10, 17, and 24 days, after the solution was filtered, and after it was heated at 56 degrees C. For the cost comparison analysis the cost of 22 patients treated exclusively with intravitreal high dose ganciclovir was compared with cost estimates for the same patients treated with a standard intravenous therapy regimen over the same time. RESULTS: There was little variation in the concentration of ganciclovir regardless of the storage conditions, suggesting that the 20 mg/ml solution was very stable. The heating and filtering experiments suggest that maximum solubility was achieved both in the freshly prepared and thawed frozen stored solution. The total cost of the intravitreal treatment was Aus $172,435 and the estimate of intravenous treatment was Aus $490,521. This represents a total saving of $318,086 (65%), or $14,458 per patient. The average number of weeks of treatment for each patient was 27.9 (613 weeks/22 patients), so the saving per patient per year was $29,946. CONCLUSION: High dose intravitreal ganciclovir therapy may be administered in a very cost effective way, which along with its acceptability, safety, and clinical efficacy make it an attractive method of treatment of CMV retinitis.

Cytomegalovirus Retinitis↗
PubMed1994

A comparison of two semi-quantitative surgical keratometers: the modified Hyde ruler and the Barrett keratoscope with "astigmatic dial".

The Hyde astigmatic ruler is an inexpensive, semiquantitative, hand-held, surgical keratometer that we modified by geometrically calculating the correct shape of its ellipses. The Barrett keratoscope is a cheap, disposable, qualitative keratometer that, unlike the Hyde ruler, produces a bright corneal image. We designed a transparent overlay, or "astigmatic dial," to use with the keratoscope. This overlay determines the magnitude of astigmatism and sets the distance the keratoscope is held from the cornea by direct comparison with the corneal image. Using a model cornea, we compared our modified Hyde ruler with the Barrett keratoscope and astigmatic dial to determine the accuracy of measurement provided by each. Both instruments had good predictive values for the true astigmatism; however, the Barrett keratoscope/astigmatic dial provided more consistent measurements of the astigmatism. We believe the keratoscope and dial provide a cheap, convenient, and accurate alternative to the more expensive microscope-mounted keratometers.

Astigmatism↗
PubMed1994

Clinical utility of the Barrett keratoscope with astigmatic dial.

Intraoperative keratometry allows some degree of control over corneal astigmatism during cataract surgery. I describe the clinical use of the Barrett keratoscope combined with an astigmatic dial that quantifies the information obtained by this simple, inexpensive, hand-held surgical keratometer. Based on a comparison of intraoperative measurements with those taken after extracapsular cataract surgery with an automated keratometer, I conclude that intraoperative keratometry reliably predicted the postoperative astigmatism. For those who had the intraocular pressure (IOP) set between 15 and 20 mm Hg intraoperatively, the mean deviation of the first postoperative measurement from the intraoperative measurement of astigmatism was +/- 1.03 D (standard error, 1.56 diopters; 95% confidence interval 0.712 to 1.35 D). When the IOP was not set, the postoperative astigmatism differed from the intraoperative reading by more than 2.00 D for 50% of the cases. Setting the IOP prior to intraoperative keratometry significantly improved the reliability of the measurement. Intraoperative keratometry by the simple device used in this study is of sufficient utility to allow the surgeon to adjust for the predicted changes in the corneal astigmatism at the time of surgery.

Astigmatism↗
PubMed1993

Prevention of intraocular pressure rise following intravitreal injection.

The intraocular pressure change produced by an intravitreal injection of ganciclovir, 2 mg in 0.1 ml, was studied in patients with cytomegalovirus retinitis. Using a Tono-pen XL to measure the intraocular pressure (IOP) of four patients (six eyes) we found the mean pressure immediately following injection was 44.5 mm Hg. Measurements taken on separate occasions after a 30 mm Hg decompression of the eye for 15 minutes before the injection showed a mean IOP of only 20.6 mm Hg after the injection. Mercury bag decompression of the eye significantly reduced the rise in IOP following intravitreal injection (difference in the mean IOP rise = 26.4 mm Hg, df = 54, t = 7.67, p < 0.001). Without ocular decompression before injection, all patients complained of temporary loss of vision, and reflux of the injection solution was frequently observed. Use of ocular decompression also reduced the discomfort of the injection. Throughout the full course of treatment by this means there were no adverse effects on the visual acuity. This technique is recommended to those performing intravitreal injections.

Cytomegalovirus Infections↗
PubMed1992

High dose intravitreal ganciclovir in the treatment of cytomegalovirus retinitis.

OBJECTIVE: To assess the role of intravitreal administration of high doses of ganciclovir as a supplement and alternative to intravenous administration in the treatment of cytomegalovirus (CMV) retinitis in patients with acquired immunodeficiency syndrome (AIDS). DESIGN: A retrospective study of visual outcome, relapse and complications of intravenous and high dose intravitreal administration of ganciclovir alone and in combination. METHOD: Twenty-three patients with AIDS and CMV retinitis (37 eyes) were examined by the authors and notes, fundal drawings and photographs reviewed. Initially patients were treated with intravenous ganciclovir alone and given supplementary intravitreal therapy for relapse or vision-threatening retinitis; however, later patients were managed with combination maintenance ganciclovir or maintenance intravitreal treatment alone. RESULTS: Relapse and loss of vision occurred frequently in patients treated with intravenous ganciclovir alone or in combination with intermittent intravitreal therapy. Eyes managed with maintenance high dose intravitreal ganciclovir alone or in combination with intravenous treatment did not relapse or lose vision. The most important complication of intravenous administration of ganciclovir was neutropenia (73% of patients), whereas that of intravitreal therapy was endophthalmitis (three eyes). CONCLUSION: High dose ganciclovir given intravitreally effectively suppressed CMV retinitis and preserved vision without adverse systemic effects or deterioration of quality of life.

AIDS-Related Opportunistic Infections↗