Lichenoid eruption due to mercaptopropionylglycine.
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Aged↗
Biomedical subjects
N Mizuno
Publications and source records attributed to N Mizuno.
Contact dermatitis due to the beta-blocker befunolol [corrected] in eyedrops.
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Adrenergic beta-Antagonists↗
Correlation between immunochemical characteristics and immunohistochemical applicability of nine lines of monoclonal antibodies against rat brain glutaminase.
Nine lines of monoclonal IgM antibodies to rat brain glutaminase were produced from a mouse. The antibodies were named MAb-19, -21, -45, -48, -51, -55, -59, -97, and -120. Immunotitration tests revealed that six of the nine antibodies (MAb-19, -21, -45, -48, -97, and -120) absorbed dose-dependently more than 75% of glutaminase activity in rat brain. By immunoelectroblotting after gel electrophoresis of the homogenate, all antibodies showed a positive band at the same position as that of the purified enzyme. However, MAb-55, -59, and -97 crossreacted with other proteins. Despite the difference in their specificities, all of the antibodies except MAb-51 competed with one another for determinants on the enzyme. The competition test further indicated that the affinities of the antibodies were in the order MAb-120 greater than -21 greater than -48 greater than -19 not equal to -45 greater than or equal to -97 greater than or equal to -55 greater than or equal to 59. When applied to immunohistochemical staining of cerebral cortex of rat brain, the five specific antibodies (MAb-19, -21, -45, -48, and -120) produced essentially the same pattern of neuronal labeling; they labeled specifically pyramidal neurons, which are reportedly glutamatergic. The most intense labeling was obtained with MAb-120.
Animals↗
Intestinal absorption of pyridoxal 5'-phosphate at physiological levels in rats.
The intestinal absorption of pyridoxal 5'-phosphate (PLP) at physiological levels (10(-7) -10(-6) M) was studied in comparison with that of pyridoxal (PL) in rat, using in vitro everted sac and an intestinal preparation that permitted continuous in situ collection of mesenteric venous blood. After PLP administration (10(-6) -10(-3) M) in situ, larger amounts of PLP were found in the mesenteric venous plasma than after PL administration at the same dose. The amount of PLP found in the mesenteric venous plasma was dependent on its dose at lower concentrations up to 10(-4) M but became independent at higher concentrations. After PL administration at various doses, the amount of PL found in the mesenteric venous blood increased linearly with the dose. When various concentrations of PLP were added to the mucosal side, under the in vitro condition with protection from alkaline phosphate hydrolysis, PLP was detected in the serosal side and the extent of PLP transport was dependent on the initial concentration of PLP in the mucosal side. When various concentrations of PL were added to the mucosal side, the extent of PL transport was independent of the initial concentration of PL in the mucosal side. In rat pretreated with actinomycin D, PLP transport in vitro was inhibited but not that of PL. N2-induced anoxia and pyridoxamine 5'-phosphate and anion transport inhibitor (4,4'-diisothiocyanostilben-2,2'-disulfonic acid disodium salt) showed no effect on PLP transport. These results suggest that PLP can be absorbed in the phosphorylated form and imply the presence of a saturable process for direct absorption of PLP itself and a diffusive process for PL absorption. In addition, the result of the in vivo neonatal experiment suggests that the neonatal intestine also can transport PLP in phosphorylated form.
Aging↗
Action spectrum for UV erythema and reproduction of the skin lesions in hydroa vacciniforme.
Action spectra for UV erythema and reproduction of the skin lesions in 4 patients with hydroa vacciniforme (HV) were studied. Vesicles were successfully reproduced by repeated daily monochromatic irradiations of 330, 340, 350 and 360 nm for 5, 6 or 7 days. The daily doses were equivalent to global solar radiation for 70-190 min. The vesicles produced were identical to those of HV, macroscopically and histologically. Repeated daily exposures to a small dose of UVA produced vesicles, whereas a large but single exposure to UVA failed to do so. Daily exposures to UVB failed to produce the vesicles. The minimal erythema dose (MED) was lower than the minimum limits of the normal persons in the 290-310 nm range.
Adolescent↗
Photosensitive lichenoid reaction accompanied by nonphotosensitive subacute prurigo caused by carbamazepine.
A 58-year-old man developed a lichenoid reaction on light-exposed areas and subacute prurigo separate from the sun-exposed areas after receiving 200 mg of carbamazepine daily for one year. The difference action spectrum for erythema with or without ingesting the drug ranged from 250 to 390 nm with peaks at 260 and 290 nm. A positive photoingestion test was obtained after administering 2 mg of carbamazepine orally in one dose. Patch test with carbamazepine was negative, while photopatch test with carbamazepine was positive. Positive intracutaneous test was obtained with an irradiated mixture of human serum albumin and carbamazepine at 24 h and 48 h after injection. This patient appeared to be a case of photosensitive lichenoid reaction accompanied by nonphotosensitive subacute prurigo caused by carbamazepine.
Acute Disease↗
Photosensitivity induced by griseofulvin.
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Aged↗
Somatotopic organization of facial nucleus of rabbit. With particular reference to intranuclear representation of perioral branches of the facial nerve.
Somatotopic organization of the facial nucleus was examined in the rabbit by the retrograde HRP (horseradish peroxidase) method; HRP was applied to the peripheral branches of the facial nerve. The facial nucleus is cytoarchitectonically divided into 5 division: the ventromedial, medial, dorsal, lateral and intermediate divisions. The ventromedial division contains neurons supplying the cervical branch. The medial division supplies the anterior auricular branch as well as the posterior auricular branch. The dorsal division is small and contains motoneurons innervating the periorbital regions through the zygomatico-orbital branchlets as well as the anterior auricular branch. Motoneurons innervating the perioral regions are numerous and distributed in the lateral and intermediate division; each of the lateral and intermediate divisions supplies both superior labial and inferior labial branches.
Animals↗
Changes of vasoactive intestinal polypeptide-like immunoreactivity in cerebrovascular nerve fibers after cervical sympathectomy in the dog.
The presence of vasoactive intestinal polypeptide-like immunoreactivity (VIP-LI) was confirmed in many neuronal perikarya of the superior cervical ganglion (SCG), as well as in many perivascular nerve fibers of the major cerebral arteries in the dog. After bilateral extirpation of the SCG, VIP-LI was decreased markedly in the basilar artery and the posterior cerebral artery, and moderately in the middle and anterior cerebral arteries.
Animals↗
Postnatal changes in the localization of prostaglandin D synthetase from neurons to oligodendrocytes in the rat brain.
Prostaglandin D synthetase (Urade, Y., Fujimoto, N., and Hayaishi, O. (1985) J. Biol. Chem. 260, 12410-12415) in the brain of 2- and 8-week-old rats was biochemically indistinguishable as judged by Mr (26,000), pI values (4.35-4.75), Km for prostaglandin H2 (approximately 20 microM), and requirement of sulfhydryl compounds. Furthermore, the enzyme was immunologically the same at both ages, as judged by immunotitration, Ouchterlony immunodiffusion, and immunoblotting analyses using a polyclonal and two monoclonal antibodies specific for the enzyme. However, the cellular localization of the enzyme was markedly different between the two ages, as examined by immunoperoxidase staining with the specific antibodies. The immunoreactivity was found in many neurons in 2-week-old rats but was not detected in most neurons in 8-week-old rats except for multipolar neurons in layers I-II of the neocortex and several clusters of dendrites in the pyriform cortex. In the adult rat, the immunoreactivity was mainly localized in oligodendrocytes. By immunoelectron microscopy, the immunoreactive deposits were seen in rough-surfaced endoplasmic reticulum and outer nuclear membrane of the glial cells.
Aging↗
Immunohistochemical demonstration of coexistence of enkephalin-and substance P-like immunoreactivities in axonal components in the lumbar segments of cat spinal cord.
Coexistence of enkephalin-like and substance P-like immunoreactivities (ENK-LI and SP-LI) was shown in axonal components in the lumbar segments of cat spinal cord by using the double immunofluorescence method. Although axonal components labeled with both ENK-LI and SP-LI were distributed throughout the spinal gray, they were most dense in the ventral horn, and most sparse in the superficial part of the dorsal horn.
Animals↗
Changes of neurotensinlike immunoreactivity in the striatum of the cat after intrastriatal injection of kainic acid.
In the course of our study on the effects of kainic acid (KA) on neuropeptidelike immunoreactivity in striatal neurons, it was found that neurotensinlike immunoreactivity (NT-LI) became visible immunohistochemically in cell bodies of some striatal neurons after injecting KA stereotaxically into the head of the caudate nucleus (Cd) of the cat. The adult cats injected with KA were allowed to survive for 1-56 days, and their brains were examined by the indirect immunoperoxidase method. In the intact Cd, NT-LI was observed in axonal elements that accumulated to form irregularly shaped patches and displayed a discrete island-and-matrix pattern. At the site of KA injection, where neuronal cell loss and gliosis were produced. NT-LI almost disappeared by the fourth day after KA injection. In parallel with these changes, many neuronal cell bodies with NT-LI became visible in striatal regions surrounding the site of KA injection. Most of these neuronal cell bodies that showed NT-LI were medium-sized and extended thick dendrites with spines; spiny processes were rarely seen on the cell bodies. On the other hand, when a lesion was placed in the head of the Cd by thermocoagulation, no NT-LI could be seen in cell bodies of striatal neurons. Thus it was presumed that NT-LI in cell bodies of medium-sized, spiny neurons of the cat striatum might be at a low level under normal conditions but enhanced in cell bodies of the neurons that survived the KA injections.
Animals↗
Quinolinic and kainic acids can enhance calcitonin gene-related peptide-like immunoreactivity in striatal neurons with substance P-like immunoreactivity.
Quinolinic or kainic acid, when injected into the cat striatum, caused neurodegenerative changes in striatal neurons. In parallel with these changes, however, calcitonin gene-related peptide-like immunoreactivity became visible in perikarya of many striatal neurons which were distributed around the lesions produced by the quinolinic or kainic acid injections. The vast majority of these lesion-spared neurons showing calcitonin gene-related peptide-like immunoreactivity were striatal neurons with substance P-like immunoreactivity.
Animals↗
Representation of the main branches of the facial nerve within the facial nucleus of the Japanese monkey (Macaca fuscata).
The facial nucleus of the Japanese monkey was divided cytoarchitectonically into the ventral, medial, intermediate, dorsal and lateral divisions. When horseradish peroxidase (HRP) was applied to the inferior labial, cervical or posterior auricular branch of the facial nerve, HRP-labeled neurons were seen in the lateral, ventral or medial division of the facial nucleus, respectively. After applying HRP to the anterior auricular-zygomatico-orbital branch, labeled neurons were observed mainly in the intermediate and dorsal divisions. HRP applied to the superior labial branch labeled neurons within the dorsal and lateral divisions.
Animals↗
Tyrosine hydroxylase-like immunoreactivity and catecholamine fluorescence in ciliary ganglion neurons.
In the ciliary ganglia of monkeys, dogs, cats and rats, some neuronal perikarya exhibited tyrosine hydroxylase-like immunoreactivity; neither dopamine-beta-hydroxylase-like immunoreactivity nor phenylethanolamine-N-methyltransferase-like immunoreactivity was found in perikarya. Some perikarya of ciliary ganglion neurons displayed catecholamine fluorescence; the fluorescent perikarya were significantly increased in number in dogs injected with nialamide. Thus the existence of dopamine-containing ciliary ganglion neurons was suggested.
Animals↗
Immunohistochemical demonstration of glutamate dehydrogenase in astrocytes.
A rabbit antiserum was raised against glutamate dehydrogenase (GDH) and applied immunohistochemically to rat brain. GDH-immunoreactive grains were observed mainly in small cells throughout the brain. In most of these cells, coexistence of GDH and glial fibrillary acidic protein was shown by an immunofluorescence method. The results suggest that GDH is primarily an astrocytic enzyme. Possible roles of astrocytic GDH in glutamate and ammonia metabolism are discussed.
Animals↗
Direct projections from the caudal spinal trigeminal nucleus to the striatum in the cat.
The results of a WGA-HRP and HRP study in the cat indicated that some neurons in the marginal zone (lamina I) of the caudal spinal trigeminal nucleus sent their axons contralaterally to the striatum; mainly to the dorsal part of the putamen, and additionally to the ventrolateral part of the caudate nucleus, at the stereotaxic rostrocaudal levels of A 13.0-A 15.5.
Animals↗
Neurotensin in projection neurons of the striatum and nucleus accumbens, with reference to coexistence with enkephalin and GABA: an immunohistochemical study in the cat.
Neurotensin-like immunoreactivity (NT-LI) was demonstrated in projection neurons of the striatum and nucleus accumbens in the cat by combining immunohistochemistry and the fluorescent retrograde neuronal labeling method. In colchicine-treated cats, many neurons with NT-LI were found in the caudate nucleus, nucleus accumbens, and putamen. Most of these neurons were medium-sized neurons with spiny dendrites. NT-LI of neuronal elements in the caudate nucleus and nucleus accumbens formed dense aggregates with irregular figures, which appeared to correspond to the striosomes of Graybiel et al. (Proc. Natl. Acad. Sci. USA 75:5723-5726, '78; Exp. Brain Res. 34:189-195, '79; Neuroscience 6:377-397, '81). Fibers with NT-LI were distributed massively to the globus pallidus and ventral midbrain regions, but not to the entopeduncular nucleus. In the ventral midbrain regions, many fine varicose fibers with NT-LI were distributed to the pars compacta and pars lateralis of the substantia nigra, ventral tegmental area, and retrorubral area. In the pars reticulata of the substantia nigra, however, fibers with NT-LI were rather sparse. Examination of consecutive sections immunostained for NT, enkephalin (Enk), GABA, and substance P (SP) revealed that 50% of neurons with NT-LI in the caudate nucleus and nucleus accumbens exhibited Enk-LI, 15% showed GABA-LI, and 5% manifested both Enk-LI and GABA-LI; no NT-positive neurons in the striatum and nucleus accumbens showed SP-LI. No morphological differences were found between NT-positive neurons with Enk-LI and/or GABA-LI and those without Enk-LI and GABA-LI. Most neurons with NT-LI in the striatum and nucleus accumbens were retrogradely labeled with True Blue injected into the globus pallidus, pars compacta and pars lateralis of the substantia nigra, and ventral tegmental area. After hemitransection severing neuronal connections between the ventral midbrain regions and the forebrain structures, fibers with NT-LI and those with Enk-LI in the ventral midbrain regions were markedly reduced in number.(ABSTRACT TRUNCATED AT 400 WORDS)
Animals↗