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Biomedical subjects

N Matsui

Publications and source records attributed to N Matsui.

At least 235 records · Page 13Linked to original sources
PubMed1984

Influence of acute diuresis on calcium balance--a comparative study of furosemide and azosemide.

The influence of acute diuresis on calcium balance induced by furosemide (FM) and azosemide (AM) was observed and compared in the human body. The treatment resulted in significant diuresis (FM: 2488 +/- 163 ml; AM: 2930 +/- 109 ml for 6 hours) and calciuresis (FM: 132.0 +/- 15.2 mg; AM: 181.0 +/- 16.6 mg for 6 hours). In spite of the urinary calcium loss, the serum calcium concentrations were elevated by both drugs. The elevation was also found even when the correction to serum protein concentrations was performed, and the magnitude of this change was less by AM than by FM. Plasma parathyroid hormone (PTH) concentrations were slightly elevated by AM only at 6 hours, but they remained unchanged by FM. This mild elevation of PTH could not be explained sufficiently by the concentration changes of serum calcium as noted above, and might be caused by factors other than serum calcium. The alkaline phosphatase concentrations corrected by means of serum protein concentrations were unchanged in both drugs, and it was suggested that the calciuresis may not extend to bone calcium remodeling at least in such a short-term experiment.

Adult↗
PubMed1984

Anterior discectomy and interbody fusion for lumbar disc herniation. A review of 350 cases.

Based on the follow-up evaluation of 350 patients in whom lumbar disc herniation was treated by anterior discectomy and interbody fusion from 1955 to 1982, 94.3% of the patients obtained bony union and justified the principle of interbody stabilization of the spine. The normalization of the myelographic pattern observed after surgery indicates that sufficient nerve decompression can be obtained by this procedure. The good clinical results may be attributed to the restoration of disc height and to the correction of spinal alignment. The good results observed in long-term follow-up evaluation of 223 patients and the fact that most of the patients resumed their former work after surgery provide further testimony of the value of interbody fusion. The procedure is indicated in young adults, physical laborers with low back pain and sciatica, and patients with spinal instability.

Adolescent↗
PubMed1983

Early pregnancy diagnosis in sows by ultrasonic linear electronic scanning.

A rapid and accurate technique for pregnancy diagnosis in sows has been developed. A portable ultrasonic linear electronic scanner was used in conjunction with 3.5 MHz multitransducers. The scanner employed ultrasonic waves to generate a two-dimensional image of an interior cross-section of an animal and the image was displayed on a TV monitor. The tip of the transducer was placed in contact with the lower flank of the standing sow about 5 cm posterior to the navel and just lateral to the nipple line directing toward the uterus. Results of tests conducted under controlled conditions on a farm, using 145 sows, proved that accuracy of the technique approaches 100% for diagnosing pregnancy from day 22 of gestation through term. The time of feeding before scanning did not affect the accuracy of pregnancy diagnosis.

Journal Article↗
PubMed1983

The effect of cortisol, progesterone, and transcortin on phytohemagglutinin-stimulated human blood mononuclear cells and their interplay.

The effect of transcortin on [3H]thymidine incorporation into phytohemagglutinin-stimulated human peripheral blood mononuclear cells and its influence on the well known suppressive effect of cortisol were investigated. Human transcortin by itself had no effect on thymidine incorporation between the concentrations of 0.25-1 X 10(-6) M. When transcortin was added to cortisol, the suppressive effect of cortisol decreased in proportion to the decrease in the protein-unbound cortisol concentration. We also investigated the influence of progesterone on transcortin-bound cortisol. When 0.5 and 1 X 10(-6) M transcortin, which contained 1 and 2 X 10(-7) M cortisol as a transcortin-bound form, were added to 5 X 10(-6) M progesterone, greater suppression of thymidine incorporation was observed that than produced by progesterone alone (86.1% and 81.3 for 0.5 and 1 X 10(-6) M transcortin, respectively). Moreover, when 5 X 10(-7) M transcortin containing the same amount of cortisol was added with 1, 2, and 5 X 10(-6) M progesterone, a greater suppression (92.6%, 74.1%, and 32.4% of control for 1, 2, and 5 x 10(-6) M progesterone) was demonstrated than that caused by progesterone alone (95.1%, 75.8%, and 49.5% of control for the corresponding concentrations of progesterone). This increased suppression was accompanied by an increase in the percentage of protein-unbound cortisol. These results indicate that unbound cortisol, whose concentration increases in the presence of progesterone, may be biologically active. The interaction between progesterone and cortisol may be modulated in part by the transcortin concentration.

Cells, Cultured↗
PubMed1983

Radioimmunoassay of human transcortin.

A specific, sensitive and simple radioimmunoassay (RIA) system for human transcortin was developed. A highly purified transcortin was prepared from pooled human serum by the following four successive steps; ammonium sulfate fractionation, cortisol-Sepharose column chromatography, Ultragel AcAA column chromatography and hydroxylapatite column chromatography. Anti-transcortin antibody was raised by immunizing rabbits. The RIA employing 125I-labeled transcortin preparation and polyethylene glycol solution for separation of free and bound-form was sensitive to transcortin in concentrations as low as 10 ng/ml. This RIA was reliable in the tests of dilution, reproducibility and recovery. The presence of cortisol does not interfere with the assay. Also a test of cross reactivity revealed that the system was not influenced by human serum albumin in a concentration 10,000 times that of transcortin. The transcortin concentrations determined by the RIA (Y) and those by the conventional steroid-binding assay (X) revealed a good correlation (Y = 1.01 X + 6.25) in normal serum, and the immunoreactivity and steroid binding activity revealed a good correlation in heat and acid-inactivated transcortin. With some total cortisol concentrations given, the transcortin concentrations were inversely correlated with protein-unbound cortisol concentrations. The present assay is useful not only for biochemical research but also for clinical studies, in which the determination of transcortin makes it possible to evaluate the concentration of protein-unbound cortisol which is the physiologically active fraction in serum.

Adult↗
PubMed1983

[Serum cortisol fractions in breast cancer].

Serum cortisol fractions were determined by isocolloidosmolar equilibrium dialysis in 93 women with breast cancer and 29 normal women. In breast cancer patients, the percentage of protein-unbound cortisol was increased at any concentration of total serum cortisol; this was accompanied by a relative decrease in the percentage of transcortin-bound cortisol. A significant increase in the unbound fraction is indicative of accelerated physiological activity of cortisol in breast cancer patients. In the breast cancer cases, there was a high incidence of obesity (61%) and impaired glucose tolerance (85%). In obese and/or diabetic patients, more remarkable increases of unbound cortisol were observed. We suggest that obesity and impaired glucose tolerance may increase the risk for breast cancer, and that the characteristic increase in the unbound cortisol fraction in women with breast cancer may reflect such risk factors.

Adult↗
PubMed1982

The serum concentrations of unbound, transcortin bound and albumin bound cortisol in patients with dysproteinemia.

Three cortisol fractions, protein-unbound (U-F), transcortin-bound (Tr-F) and albumin-bound cortisol (Al-F) were measured in patients with dysproteinemia by a newly devised isocolloidosmolar equilibrium dialysis method. Total cortisol (Total-F) concentrations in patients with liver cirrhosis (LC), anorexia nervosa (AN) and cachexia due to cancer (CA) were higher than in normal subjects, and those in patients with nephrotic syndrome (NS) and multiple myeloma (MM) remained within the normal range. In all groups of patients, the U-F concentration, which is believed to be the sole active fraction of cortisol, showed significantly higher values than in the normal subjects. We, therefore attempted to find which of the two binding proteins contributes to the elevated U-F concentrations. Concentrations of each cortisol fraction are greatly changed by alterations in the Total-F concentration. We therefore compared the Tr-F against Total-F and Al-F, and U-F against Total-E of patients with those of normal subjects. It was found that decreased transcortin-binding and not albumin-binding in the patients with cirrhosis, nephrotic syndrome and myeloma contributed to an increase in the U-F concentration. Although decreased binding of albumin due to hypoalbuminemia was found in LC, NS, MM, CA and AN, it had relatively little effect on cortisol distribution in the serum.

Anorexia Nervosa↗
PubMed1982

The role of heparin in the changes of lipid patterns during a single hemodialysis.

The effects of heparin administered during dialysis on serum lipids and lipoproteins were evaluated by comparing dialysis with heparin and dialysis without heparin. Gabexate mesilate (GM), a synthetic proteinase inhibitor, was used as an anticoagulant during dialysis without heparin. In dialysis with heparin, significant increases were observed in free fatty acids (FFA), high-density lipoprotein cholesterol (HDL-C), and plasma lipolytic activity (PLA). Triglyceride (TG) was decreased reciprocally and the lipoprotein electrophoretic pattern was markedly modified. In contrast, in dialysis without heparin, no significant changes were observed in all parameters of lipid metabolism. These data suggest that dialysis induced changes in lipids are mainly caused by heparin and that acetate and glucose in dialyzate make an almost negligible contribution to abnormal lipid patterns in patients on maintenance hemodialysis.

Electrophoresis, Agar Gel↗