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Biomedical subjects

N Malla

Publications and source records attributed to N Malla.

52 records · Page 3Linked to original sources
PubMed1986

Comparative evaluation of enzyme-linked immunosorbent assay for the diagnosis of pulmonary echinococcosis.

An enzyme-linked immunosorbent assay was done for the detection of immunoglobulin G and M (IgG and IgM) antibodies to Echinococcus granulosus in surgically proved cases of hydatidosis, especially pulmonary hydatidosis, by use of human hydatid cyst fluid antigen and soluble scolex antigen. This assay was compared with the following standardized techniques: the indirect hemagglutination test, the indirect immunofluorescent antibody test, and Casoni's intradermal test. The enzyme-linked immunosorbent assay, with either of the antigens (human hydatid cyst fluid or soluble scolex antigen), was more sensitive and specific than the other techniques in diagnosing cases of hydatidosis, especially hydatid disease of the lung.

Antigens, Helminth↗
PubMed1986

Effect of serotonin on T lymphocyte proliferation in vitro in healthy individuals.

The direct effect of different concentrations of serotonin on T lymphocyte proliferation in vitro was seen in 20 normal individuals. It has been observed that the neurotransmitter had a suppressive effect on mitogen-induced lymphocyte proliferation. This study shows that the effect of serotonin on the immune system is direct.

Cells, Cultured↗
PubMedDate not supplied

Parasitic diarrhoea.

Explore the source record for details and available documents.

Acquired Immunodeficiency Syndrome↗
PubMedDate not supplied

Evaluation of a 200-kDa amastigote-specific antigen of L. donovani by enzyme-linked immunosorbent assay (ELISA) for the diagnosis of visceral leishmaniasis.

A purified 200-kDa antigenic fraction from Leishmania donovani axenic amastigotes was evaluated by ELISA for the detection of antibody response in visceral leishmaniasis (VL) patients, post kala-azar dermal leishmaniasis (PKDL) patients and controls, for the diagnosis of visceral leishmaniasis. A positive antibody response to the 200-kDa amastigote fraction and to Leishmania amastigote soluble antigen (LASA) was found in 29 (96.6%) and 30 ((100%) confirmed VL patients, respectively, by the use of ELISA. However, only 1 (10%) out of 10 PKDL patients had detectable antibody response to 200-kDa fraction while all the 10 (100%) PKDL patients exhibited an immune response to LASA. Therefore, use of the 200-kDa antigenic fraction for the detection of antibody response in an ELISA follow-up (post treatment) of VL patients may have prognostic significance, and it may also be useful for differentiating active VL and PKDL.

Animals↗
PubMedDate not supplied

Cytokine mRNA expressions in symptomatic vs. asymptomatic amoebiasis patients.

Infection with Entamoeba histolytica results in high mortality worldwide. Studies on the cytokine response in symptomatic and asymptomatic amoebiasis (caused by E. histolytica, the pathogenic species and E. dispar, the non-pathogenic species) subjects and their correlation with symptomatology are lacking. The present study reports the cytokine response (TNF-alpha, IFN-gamma, IL-4, IL-10 and TGF-beta) in such subjects as measured by RT-PCR. The results showed significantly (< 0.05) higher expressions of IL-10 and TGF-beta in the symptomatic group as compared to the asymptomatic and healthy controls. The cytokine profile indicated the role of suppressive immune response in symptomatic amoebiasis patients.

Adolescent↗
PubMedDate not supplied

Cryptosporidiosis in a tertiary care hospital.

BACKGROUND: Cryptosporidium, an important cause of diarrhoea, has been reported worldwide both in immunocompetent and immunocompromised individuals and has emerged as a serious public health problem. This study was undertaken to assess the present status of cryptosporidiosis in children and adults with diarrhoea who attended the Nehru Hospital, Chandigarh which is a tertiary care hospital. METHODS: Routine stool examination was done using saline and iodine stained preparations for various parasites. Modified Ziehl-Neelsen and rapid safranin-methylene blue techniques were used to detect Cryptosporidium in 2000 stool samples (1645 adults, 355 children) from March to November 1998. RESULTS: Of the 2000 samples, 205 (10.2%) were positive for various parasites. Five (1.4%) children were positive for Cryptosporidium and one child was positive for human immunodeficiency virus. In adults, Cryptosporidium was found in only one patient (0.06%). Giardia lamblia was the commonest parasite detected both in adults (4%) and children (15.2%). CONCLUSION: The present study highlights the importance of Cryptosporidium as a cause of diarrhoea, especially in children. Thus, there is a need for specific staining techniques to detect Cryptosporidium in routine diagnostic laboratories.

Adult↗
PubMedDate not supplied

Cryptosporidiosis and related diseases.

Cryptosporidium, is a parasite classified as a member of the class Sporozoea and Subclass Coccidia. The other parasites which are grouped under the Coccidia Subclass are Isospora, Sarcocystis, Plasmodium, Piroplasmia and Microspora. Cryptosporidium, Isospora, Sarocystis and recently some of the Microspora have been observed to cause infections of the gastrointestinal tract. This review summarizes the recent developments in our knowledge about these parasites especially those which are of medical importance.

Cryptosporidiosis↗
PubMedDate not supplied

Increasing toxoplasma seropositivity in women with bad obstetric history and in newborns.

BACKGROUND: Most infants with congenital Toxoplasma gondii infection have no symptoms at birth but few may develop retinal diseases or neurological abnormalities later in life. The presence of significant titres of antitoxoplasma antibodies in women in the reproductive age group indirectly indicates that Toxoplasma gondii is the cause of such congenital abnormalities and also sporadic abortions in some women. METHODS: We did a retrospective analysis of antitoxoplasma antibodies detected by indirect haemagglutination assay, in women with bad obstetrical history and in newborns clinically suspected of congenital toxoplasmosis during 1981-91. RESULTS: A significant increase in seropositivity in women and newborns was seen during 1989-91 as compared to 1981-88. More seropositive patients were recorded between April-June and October-December. However, no significant correlation could be observed between rising incidence of seropositivity and the seasonal distribution or age of women. CONCLUSION: Epidemiological studies are required to ascertain the reason for the increasing trend of toxoplasma seropositivity and to suggest appropriate control strategies as it is possible to prevent congenital infection.

Adolescent↗