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N Müller

Publications and source records attributed to N Müller.

355 records · Page 20Linked to original sources
PubMedDate not supplied

The repetitive microtubule-associated proteins MARP-1 and MARP-2 of Trypanosoma brucei.

The microtubular membrane skeleton of Trypanosoma brucei contains two closely related, repetitive, high-molecular-weight microtubule-associated proteins, MARP-1 and MARP-2 (MARP for Microtubule-Associated Repetitive Proteins). Their structure is unusual in that they consist of tandemly arranged, strongly conserved 38-amino-acid repeat units over almost their entire length of about 320 kDa. Their nonrepetitive N and C ends are comparatively short. The predicted amino acid sequences reveal a gradient of similarity between MARP-1 and MARP-2 which increases from the N-terminus (no significant similarity) through the repeat domain (50% similarity) to the C-terminus (94.5% similarity). Transfection of mammalian cell lines with recombinant fragments of MARP-2 demonstrate that the nonrepetitive C-terminus of MARP-2 binds specifically to microtubules. This C-terminus does not show sequence similarity with any other microtubule-associated proteins and thus appears to represent a novel type of microtubule-binding domain.

Amino Acid Sequence↗
PubMedDate not supplied

The influence of N7 substituents on the stability of N7-alkylated guanosines.

Guanosine alkylated at the 7-position readily decomposed by imidazole ring opening and depurination. There is a direct correlation between the electron-withdrawing effects, expressed as the Taft substituent constant sigma*, of various alkylsubstituents in 7-position and the respective acid dissociation constants and chemical stabilities of 7-alkylated guanosines. The respective values for the slopes of the regression lines were rho* = +1.89 for imidazole ring opening and rho* = +0.175 for the depurination reaction. Unknown Taft constants can be determined from the sigma*/pKa regression line.

Alkylation↗
PubMedDate not supplied

A comparison of two phonological profiles: a tutorial.

The notion of "linguistic profile" as an alternative to standardized testing for speech and language disorders is discussed in this tutorial. Two specific examples of profiles, both for disordered phonology, are described, and readers are guided through their use. Finally, their relative strengths and weaknesses are assessed.

Humans↗
PubMedDate not supplied

Melanoma-associated adhesion molecule MUC18/MCAM (CD146) and transcriptional regulator mader in normal human CNS.

The proteins MUC18 and Mader have been identified as markers of tumor progression in melanoma cells. MUC18, also known as MCAM (melanoma cell adhesion molecule) and as CD146 (endothelial antigen), is a cell adhesion molecule belonging to the immunoglobulin superfamily. Mader is a transcriptional regulator shown to negatively regulate EGR-1. As it is known that neoplastic cells of neuroectodermal origin frequently express neuron-specific molecules, we studied whether these melanoma-associated antigens are found in normal CNS tissue. We investigated the expression of MUC18/MCAM and Mader in adult human post mortem CNS tissue by immunohistochemistry, immunoblot and two-dimensional gel electrophoresis. Our results show that Mader is preferentially expressed on neurons and glial cells and that the adhesion protein MUC18/MCAM is mainly expressed on vasculature within the CNS. These observations may have important implications for further studies investigating their possible roles in cell adhesion and proliferation control within the CNS.

Adult↗
PubMedDate not supplied

Expression of T cell receptor gamma delta antigens in human brain tissue.

The human gamma delta T cell receptor (TCR) is normally expressed on lymphoid tissue. Since expression of different molecules of the T cell system has been described for human brain cells, we examined the expression of CD4 and TCR gamma delta antigens with a panel of various anti-gamma delta TCR monoclonal antibodies (mAbs) and an anti-CD4 mAb using immunohistochemistry on different regions of frozen human postmortem tissue from five different brains. We could confirm the expression of CD4 antigens. gamma delta TCR expression on brain tissue was found in different regions of the brain by immunohistochemistry. Double staining with anti-gamma delta TCR and antineuronal enolase (NSE) mAbs showed that gamma delta TCR+ cells were not stained by anti-NSE, although they were sometimes located near neurons and showed dendritic forms; they are possibly tissue-resident gamma delta T cells, as described in the skin. Polymerase chain reaction analysis using a highly sensitive primer sequence against the constant-region delta sequence supports, combined with immunohistochemistry findings, the notion that the gamma delta TCR is expressed in human brain.

Adult↗
PubMedDate not supplied

Detection of the novel cell adhesion molecule MUC18 in human brain tissue.

The implication of cell adhesion molecules (CAMs) in the mediation of the immune response to inflammatory stimuli has been shown in different neurological syndromes. The neural cell adhesion molecule (NCAM) and the myelin associated glycoprotein (MAG) are functionally important CNS-cell adhesion molecules (CAMs) and members of the immunoglobulin gene superfamily (IgSF). Both CAMs are expressed from the time of neuronal induction and myelin formation and are likely to contribute to the generally adhesive properties of neurons and oligodendrocytes. The present study describes, by means of SDS-PAGE and immunoblotting, a novel CNS antigen which is a glycoprotein detected in post mortem frontal lobe tissue of ten subjects at an M(r) 65 and 113 kD which showed the greatest sequence homology to neuronal IgSF members like NCAM. This expected membrane CAM, with probable functions in CNS cell-cell interactions, had been detected first in primary human melanoma cells, with increasing expression as tumors progress and is expected to be a developmentally regulated CAM in neuroectodermal tissues.

Animals↗
PubMedDate not supplied

[Pathohistological findings in palatine glands in autopsy and biopsy material].

Histological examinations of the palatine glands were performed in 100 postmortem cases and 65 biopsies of outpatients. These data were related to personal characteristics, such as age, sex, denture-wearing and certain primary diseases. The histological results of the postmortem material were found to be only partly comparable to those of the biopsy specimens. The pathohistological findings in the parenchyma of the outpatients proved to be less pronounced in the individual groups than those of the postmortem cases. An evaluation of the postmortem findings showed inflammatory, ectatic and metaplastic alterations of the ducts as well as atrophies and inflammations of the parenchyma, particularly in baseplate denture wearers and with increasing age. In persons under 65 the tissue irritation caused by baseplate dentures resulted in increased gland and duct alterations. In the dead above 65 the difference between age-dependent and denture-induced alterations of the parenchyma was statistically immaterial.

Aged↗
PubMedDate not supplied

[Autologous blood transfusion in elective operations. Procedures with 102 blood retransfusion cases].

Since June 1987 a total of 102 patients undergoing elective operations have donated autologous blood for a later possible retransfusion. Although autologous transfusion was time-consuming, nearly all patients accepted this method because they received the safest of all possible transfusions. This way, preoperative psychological anxieties could be reduced and it was made easier for the patients to decide in favor of the operation. Severe somatic pains caused by blood donation were not observed.

Adolescent↗
PubMedDate not supplied

[Crisis intervention in the framework of an acute psychiatric general ward].

Basing on the description of the concept for treating patients admitted and treated on the lines of crisis intervention for acute psychogenic crises, in an open, unsegregated (i. e. for both sexes) general psychiatric ward for acute cases in a university hospital, the data collected from 90 patients in the course of a year in respect of age, sex, cause of crisis, suicidal tendencies, diagnosis and duration of stay and follow-up treatment were analysed and compared with those collected by other crisis intervention units. It was found that there was major agreement regarding most criteria between our unit and other units; however, neurosis or personality disorder were diagnosed much more frequently than a psychogenic reaction. Over and above this, the inpatient treatment period was longer. We believe that the differences are due to an understanding of the crisis concerned which classifies the patients not so much as healthy persons thrown off balance by a major upset, rather as patients with chronic psychic difficulties that decompensate through various external factors. In the first place, treatment of the basic disease intends to protect the patients against re-exacerbation of unpleasant conflicts that are of a piercing nature. The article also points out the pros and cons of a ward to which crisis patients and psychiatric emergency cases are admitted without previous selection and can be treated adequately without additional stress being placed on the patient by a transfer or a change of physician and therapist.

Adult↗