Search PubMed⌕ Search

Biomedical subjects

N Müller

Publications and source records attributed to N Müller.

At least 307 records · Page 17Linked to original sources
PubMed1983

Age dependent phagocytosis of red cell membranes by the reticulohistiocytary system of the isolated perfused rat liver.

Isolated rat livers were perfused with a medium free of immune globulines, protein and hemoglobin to investigate age-dependent differences in the phagocytosis of erythrocyte membranes. Membranes of young erythrocytes were phagocytised in a significantly higher rate than those of old ones. The control parameters of the liver function were also significantly higher during the increased phagocytosis of the young cells than that of old cells and cells of the control group.

Aging↗
PubMed1982

Cloning of mglB, the structural gene for the galactose-binding protein of Salmonella typhimurium and Escherichia coli.

From libraries of EcoRI fragments of Salmonella typhimurium and Escherichia coli DNA in lambda gt7, phages could be isolated that carry mglB, the structural gene of the galactose-binding protein as well as other mgl genes. Lysogenization of an E. coli mutant carrying a defective galactose-binding protein with lambda gt7 mglB (Salmonella) restores full galactose transport and galactose chemotaxis. Both the E. coli mutant protein as well as the wild-type Salmonella galactose-binding protein are synthesized in this strain. The EcoR1 fragments of both organisms carrying the mgl genes were 6 Kb long. They were subcloned into the multicopy plasmid pACYC184. The hybrid plasmid containing the Salmonella mgl DNA gives rise to the synthesis of large amounts of galactose-binding protein in the periplasm of E. coli. The protein can be precipitated by antibodies against the E. coli binding protein and is identical to the fully processed protein isolated from Salmonella typhimurium LT2. In vitro protein synthesis (Zubay-system) with either lambda gt7 mgl phages as well as the hybrid plasmid as DNA matrix produces the galactose-binding protein mainly in precursor form that is precipitable by specific antibodies.

Bacteriophage lambda↗
PubMed1982

[Amniotic fluid embolism with coagulation disorder--a case report (author's transl)].

A case of amniotic fluid embolism during the delivery of a twin pregnancy is reported. After immediate resuscitation with artificial ventilation the mother and the neonates survived the acute episode. As a result of hyperfibrinolysis with premature separation of the placenta a dramatic postpartum haemorrhage occurred, which could be controlled by Fritsch' manoeuvre. Meanwhile it was possible to restore the coagulation disorder with fresh whole blood, fibrinogen and an antifibrinolytic agent (Aprotinin).

Aprotinin↗
PubMed1982

Complement, coagulation and fibrinolytic parameters in hereditary angioedema (HAE).

The intrinsic clotting, the kinin generating and the fibrinolytic systems were investigated in 10 patients with hereditary angioedema (HAE), 10 patients with chronic urticaria and 18 healthy volunteers. In spite of the fact that patients suffering from HAE severely lack C1 INH, neither the intrinsic coagulation nor the fibrinolytic systems are impaired. There was a slight decrease of plasma kallikrein--as already known--and moreover a greater decrease in HMW-kininogen, and increase in Factor XII levels. Furthermore, activation of pre-kallikrein was delayed in these patients. These findings make it apparent that lowered HMW-kininogen levels compensate the lack of C1 INH, thus preventing an enhanced activation of the intrinsic clotting and the fibrinolytic systems.

Angioedema↗
PubMed1981

[Effect of heparin in uremic patients during hemodialysis].

A sufficient level of heparin was maintained during hemodialysis by heparinization with initially 5000 I.U. and subsequently 1500 I.U./hour continuously. Active heparin amounted 70% of total heparin during the entire procedure. The elimination rate of heparin increased fivefold in patients with a decrease of the albumin-globulin ratio. The antithrombin III level was found to be slightly reduced. Levels of Hageman-factor as well as fibrinogen remained constant during the dialysis procedure.

Adolescent↗
PubMed1981

Factor XII assay with the chromogenic substrate chromozym PK.

A chromogenic assay for the determination of factor XII using the chromogenic substrate Chromozym PK was evaluated. The assay was linear in the range 10 U/l to more than 200 U/l. Using the assay, the normal range of factor XII (50 healthy volunteers at random) was 136 U/l +/- 27 U/l. Kallikrein-inhibiting concentrations of aprotinin did not influence factor XII. Comparison of the chromogenic with the clotting assay resulted in a correlation coefficient of r = 0.831 (p-value less than 0.001). In patients with deep vein thrombosis, factor XII level was found to be reduced to about 60% of normal activity.

Adult↗
PubMed1980

Coagulation parameters and heparin levels in open-heart surgery.

Coagulation parameters were studied during open-heart surgery. Severe reduction of the platelet count and of several other clotting factors was observed. The half-rate of heparin varied threefold in the patients. This may indicate that the dosage of protamine chloride prior to the end of the surgical procedure has to be adjusted to the individual heparin levels.

Antithrombin III↗
PubMed1980

[Coagulation properties of the extravascular fluid. I. Coagulation factors in thoracic-duct lymph].

All coagulation factors could be found in the thoracic duct lymph of dogs, but the coagulation activities are significantly lower than that of plasma. Analysis of thoracic duct lymph shows that the activity components are significantly lower for factors I (30%), II (60%), V (20%), VII (50%), VIII (30%), IX (25%), X (70%) and factor XIII (30%). With global (TEG) and group test (PTT, thrombin time) various coagulation properties could be detected in blood and lymph. Antithrombin III reached two third of the plasma concentration. The prothrombin consumption in lymph was markedly reduced. The most remarkable result appears to be a certain quantitative modification of the individual factors in the lymph in comparison to those in blood. Although the lymph does not contain any thrombocytes, thromboplastin formation is sufficient for clot formation.

Animals↗
PubMed1980

[Coagulation properties of the extravascular fluid. II. Components of the fibrinolytic system in thoracic-duct lymph].

Only little is known about the activity of the fibrinolytic factors outside the vascular space. Therefore fibrinolytic activity was measured in blood and thoracic duct lymph of dogs. The results are summarised as follows: With regards to the fibrinolytic rates, the extravascular spaces take on a special position: There is a profibrinolytical total constellation.

Animals↗
PubMed1979

[The development of transfusion medicine (author's transl)].

The development of transfusion medicine started from the attempts to transfuse blood in animals in the 17th century and the first fruits were seen in the second half of the 19th century. About 1800, however, Blundell introduced a new development which brought fundamental changes with the elucidation of the problems of defibrination, the phenomenon of hemolysis and coagulation. At the beginning of the 20th century the serological problems were solved by Landsteiner's discoveries. The introduction of anticoagulation permitted the conservation of blood and led to the discontinuation of direct blood transfusion in favour of indirect transfusion of conserved blood. This considerably enlarged the functions and possibilites of transfusion medicine which opened into the development of blood component therapy with its opportunities for optimal care of the patients.

Animals↗
PubMed1979

[Lymphocytotoxic antibodies in cervical cancer].

Sera of 120 patients, suffering from cervical cancer (CCa) of different clinical stages (stage 0: n = 27; stage I: n = 29; stage II--IV: n = 64) as well as the sera of 116 healthy individuals as control group were examined for lymphocytoxic antibodies by microlymphocytotoxicity tests, variated in incubation temperatures (15 degress -- 22 degrees -- 37 degrees C) and incubation times (30--180 minutes). At an incubation temperature of 15 degrees C cold reacting lymphocytotoxic antibodies were detected in 26 patients' sera (21.7%) and in 13 control sera (11.2%). At an incubation temperature of 22 degrees C 36 CCa-sera (30%) were positive in comparison to 11 sera of the control group (9.5%). At an incubation 37 degrees C 40 CCa-sera (33.3%) and 7 control sera (6%) were positive. Relating these results to the clinical stages of the carcinoma, the highest percentage of lymphocytotoxicity was found in the sera of patients with an early stage of the disease. Increased evidence of lymphocytotoxic antibodies in the sera of patients with cervical carcinoma supports the assumption, that various immune reactions may be involved in this disease.

Animals↗
PubMed1978

[Humoral immune response--cervical cancer patients: lymphocytotoxic antibodies (author's transl)].

Sera of 120 cervical cancer patients as well as that of 116 healthy control persons were screened for lymphocytotoxic antibodies using microlymphocytotoxic tests variated in temperature and incubation time. In 76 of 120 cancer-sera (63.3%) lymphocytotoxic antibodies could be detected in comparison with 26 sera of 116 controls (22.4%); p less than 0.0005. With the differentiation into the stages of the disease the highest percentage of lymphocytotoxic antibodies was found in patients with stage 0 of cervical cancer. The presence of lymphocytotoxic antibodies in sera of cervical cancer patients suggest the possibility that this disease is an allotransplant as well as expression of an autoimmune process.

Antilymphocyte Serum↗
PubMed1977

[HL-A-B 27 in Reiter's disease with special reference to oligosymptomatic course forms].

In 21 patients with the clinical suspicion of oligosymptomatic R.S.), 21 patients with the clinical suspicion of oligosymptomatic R.S. and 16 patients with recurrent non-gonococcal urethritis 23 HLA-antigens were assessed using a lymphocyte toxicity test. 413 blood donors served as a controll group. 85,7% of the patients with R.S. had phenotyp HLA-B 27 compared with 7,4% of the controll persons. The group with the suspicion of R.S. exhibited with 57,1% a marked increased incidense of HLA-B 27 as well. Compared to this, the HLA-B 27 frequency in the patients with recurrent non-gonococcal urethritis was with 6,3% in especially of its oligosymptomatic courses. Because of the close association between HLA-B 27 and R.S. determination of histocompatibility antigens proved to be a valuable tool in the diagnosis of Reiter's disease, especially of its oligosymptomatic courses.

Adolescent↗
PubMed1977

[HL-A B 27 a criterion for Reiter's syndrome].

The prevalence of 23 HLA antigens were examined in 21 patients with a typical Reiter's disease, 21 patients with incomplete Reiter's syndrome as well as 16 patients with non-gonococcal urethritis. HLA-B 27 antigen was found in 85,7% of patients with typical Reiter's disease as compared to 7,4% of 413 healthy controls. HLA-B 27 was also detectable in 57,1% of patients with clinical suspicion of Reiter's disease. No significant difference from the control frequencies of HLA specifities was found in patients with non-gonococcal urethritis. The association between HLA-B 27 and Reiter's syndrome is so marked that tissue typing is a valuable adjunct in the diagnosis of Reiter's syndrome.

Arthritis, Reactive↗
PubMed1977

[HL-A polymorphism and its relation to cervix neoplasms].

Twenty-three HLA antigens were investigated in 121 patients with clinical cancer as well as 188 male and 212 female controls. Patients with cervical carcinoma showed significant differences in some HLA frequencies from that of controls. Patients with cervical carcinoma showed significant differences in some HLA frequencies from that of controls. In the localized form of the neophasia HLA-A3 was decreased, HLA-A9 and B 12 were increased. In the disseminated form of cervical carcinoma HLA-A 1 were found decreased and AW 32 and B 12 increased. HLA antigens could be factors, together with others yet unknown, i.e. immune response genes, HSV-2-infection, which produce an increased susceptibility to the disease.

Antibody Formation↗
PubMed1977

[Cold-lymphocytotoxic antibodies in the sera of cervix-carcinoma patients].

Sera of 120 patients with cervical cancer were screened for cold reacting, complement dependant autolympho-cytotoxins (CoCoCy). These antibodies occurred in 21,7% of the patients in comparison with 11,2% of 116 controls. The highest percentage of CoCoCy-antibodies was detected in the sera of patients with cervical cancer stage I (27,6%). Thus demonstration of CoCoCy-antibodies may be of pathogenetic rather than of diagnostic interest. CoCoCy-antibodies could be seen to reflect autoimmune reactions in cervical cancer.

Autoantibodies↗