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Biomedical subjects

N M Trofimov

Publications and source records attributed to N M Trofimov.

17 recordsLinked to original sources
PubMed1985

Sedimentation analysis of the RNAs isolated from interfering particles of Lassa and Machupo viruses.

Vero cells chronically infected with Lassa virus as well as BHK-21 cells chronically infected with Machupo virus produced particles interfering with the replication of homologous, but not heterogeneous viruses. RNAs of standard virus particles contained 4 sedimentation classes of molecules: 28-31 S, 22-24S, 18 S and 4-6 S. The 28-31 S molecules were not present among the RNAs of interfering particles of Lassa and Machupo viruses.

Animals↗
PubMedDate not supplied

[Properties of influenza virus strains causing the influenza epidemic in Moscow in 1972-3].

The antigenic and some biological properties of influenza virus strains isolated during 1972-1973 epidemic were studied. Altogether 114 strains were isolated from sick infants of the first months of life beginning from neonatality. The strains under study were found to have high adaptation and elution activity, to be highly sensitive to inhibitors and to produce polymorphous allantoic population in the adaptation period. The hemagglutinating and neuraminidase components were shown to be fairly closely related to those of the membrane of the reference A2/Hong Kong/1/68 strain (H3N2). At the same time, a low neutralizing activity of the antisera for this strain against Moscow strains of 1972-1973 in biological neutralization tests in chick embryos was observed, explaining very high susceptibility of the population to influenza type A infection in the winter of 1972-1973. The strains under study were antigenically homogenous and most closely related to the reference A2/England/42/72 (H3N2) strain.

Antigens, Viral↗
PubMedDate not supplied

[Cultivation of reovirus in human embryonic organ cultures].

Human embryo lung and intestine organ cultures were infected with reovirus type 3 isolated from the lungs of a newborn baby. In the lung organ culture the virus multiplied quite intensively producing in the bronchi characteristic 4-6 nuclei cells.

Cytopathogenic Effect, Viral↗
PubMedDate not supplied

[Experimental study of the influence of reovirus infection on the course and outcome of pregnancy].

The possibility of intrauterine infection of fetuses of random-bred white mice with reovirus type 3 at various periods of pregnancy has been demonstrated. The unfavourable effect of reovirus infection on the course and outcome of pregnancy in some cases was manifested by impairment of embryogenesis, stillbirths and subsequent death of defective offsprings. Morphological examinations revealed marked pathological alterations in the lungs, brain, liver, and kidneys of suckling mice infected in utero.

Animals↗
PubMedDate not supplied

[Chronic infection of BHK-21 cells by the Machupo virus. The development of the system and properties of the virus produced by cells of this line].

A new model of chronic infection of BHK-21 cells with Machupo virus has been obtained. The chronically infected culture of BHK-21-M cells differed morphologically from the control culture by the presence of giant mono- and multinuclear forms with granular cytoplasm and by an increase in the number of small rounded cells. In the system of BHK-21-M cells, the persisting virus was produced permanently but the per cent of infected cells determined by indirect immunofluorescence and the infectious centre method was not high (12-17%). The main factor contributing to the development of Machupo virus persistence in this cell system appears to consist in the formation of defective interfering particles.

Animals↗