Biomedical subjects

N L Ol'sinskaia

Publications and source records attributed to N L Ol'sinskaia.

4 recordsLinked to original sources

PubMed1987

[Search for propionic acid bacteria in the human intestine].

The possibility of detecting propionic acid-producing bacteria in the intestine of healthy humans with a view to obtaining a strain which is physiologically most suitable for therapeutic purposes has been studied. The selective conditions for the isolation of propionic acid-producing bacteria from the large intestine have been experimentally established. Analysis of 70 puncture biopsy specimens of parietal mucus and luminal contents from different sections of the intestine has not shown the presence of the representatives of propionic acid-producing bacteria. The strains isolated under the conditions selective for such bacteria have been found to belong to the family Enterobacteriaceae. These strains have proved capable of synthesizing vitamin B12, but the synthesis of propionic acid has not been observed.

Adult↗

PubMedDate not supplied

[Formation of primary alcohols and palmitic acid in the microbiological oxidation of hexadecane].

Forty seven microbial strains oxidized hexadecane to form primary cetol and palmitic acid. The maximum quantitiy of the compounds was accumulated in the exponential phase of growing cultures (for 72 hours) and for 6 to 16 hours of incubation of resting cell suspensions. Candida yeast were shown by the gas-liquid chromatography method to be the most active producers of cetol.

Alcohols↗

PubMedDate not supplied

[Paraffin oxidizing system of the yeast Candida guilliermondii].

It has been demonstrated that the enzyme system of Candida guilliermondii responsible for hydrocarbon oxidation involves NADPH-cytochrome c-reductase (EC 1623) and cytochrome P-450. The system is located in the microsomal fraction. Cytochrome P-450 synthesis is induced by hexadecane occurring in the medium. The cellular content of cytochrome P-450 varies in the course of the culture growth. There is a correlation between the cellular content of cytochrome P-450 and the synthesis of primary products of hexadecane oxidation.

Candida↗

PubMedDate not supplied

[n-Alkane oxidation by propionic acid bacteria].

Propionic acid bacteria were found for the first time to be capable of oxidizing n-alkanes. The value of QO2 is higher on a mixture of n-alkanes (C12--C20) than on individual hydrocarbons whose availability is determined by the length of a hydrocarbon chain: C15 and C16-alkanes are oxidized best of all; C14, C13 and C12-alkanes are oxidized at a lower rate; and alkanes whose chain comprises less than ten carbon atoms are not oxidized at all. Hexadecane is oxidized to CO2, cetyl alcohol and palmitic acid being intermediates; this suggests the existence of the monoterminal pathway for oxidation of n-alkanes in propionic acid bacteria.

Alkanes↗