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Biomedical subjects

N Kurihara

Publications and source records attributed to N Kurihara.

At least 253 records · Page 14Linked to original sources
PubMed1978

Effect of dimercaptosuccinic acid on toxicity of Habu snake (Trimeresurus flavoviridis, Hallowell) venom.

Habu venom (HV) incubated with dimercaptosuccinic acid (DMS) or thiosuccinic acid (TMA), was given subcutaneously to mice. The LD50 of HV was 19.7 mg/kg, and after treatment with TMA and DMS, it was 38.1 and 47.1 mg/kg, respectively. The logarithm concentration--mortality curve for the DMS-treated HV shifted to the right compared with the curve for crude HV. The curve for the TMA-treated HV was intermediate between these two. Decimal two mg of HV was then incubated with DMS, and such was given intramuscularly to mice. Hemorrhage and edema which occurred after administration of crude HV were markedly decreased with injection of DMS-treated HV, but the myonecrosis was not greatly affected. At 24 hr after the intramuscular dosing of 1 mg of HV into rats, lymphopenia, acute involution of lymphatic tissue, decrease in liver glycogen and lipid granules in zona fasiculata were apparent, indicating the mark of a stressor. When the DMS-treated venom was administered, the blood picture and histological findings did not differ greatly from the controls. As DMS has marked anti-HV activity, it may be used to investigate treatments for cases of Habu snake bite.

Adrenal Cortex↗
PubMed1976

Carcinoembryonic character of gamma-glutamyltranspeptidase in primary hepatocellular carcinoma.

As a characteristic isoenzyme of gamma-glutamyl transpeptidase (gamma-GTP) has been detected in sera of patients with primary hepatocellular carcinoma, which migrates to alpha-globulin region on polyacrylamide-gel electrophoresis, biochemical studies on human fetal liver, Morris hepatoma (7316-A, 7794-A), and human hepatoma was carried out to elucidate its carcinoembryonic character. The highest distribution of the enzyme was found in particle fraction of human fetal liver as well as of Morris hepatoma, and an isoenzyme of gamma-GTP with the same electrophoretic mobility as detected in human hepatoma was obtained, which reflected to the pattern of the serum zymograms. Histochemically, the enzyme was distributed in plasma membrane of the fetal hepatocytes and Morris hepatoma cells, while it was distributed diffusely throughout the cytoplasm of human hepatoma cells. These findings may strongly suggest that gamma-GTP in hepatoma has a carcinoembryonic character and the detection of a serum isoenzyme in the alpha-globulin region is a quite diagnostic as well as the detection of alpha-fetoprotein in the field of neoplasma of the liver. The physicochemical and kinetic properties of the enzyme in human hepatoma were also discussed.

Animals↗
PubMed1975

Stability in lobar ventilation distribution during change in thoracic configuration.

Stability in lobar ventilation was examined in dogs during bilateral electrophrenic respiration (BEPR) and positive pressure-assisted ventilation (PPAV). Bilateral prior ligation of the lower and middle lobe pulmonary arteries monitoring of upper lobe ventilation as alveolar minute ventilation (VA), middle and lower lobe ventilation as dead space (VD), and VD/VT ratio, both calculated by the Bohr equation. As documented by chest films, transverse and anteroposterior thoracic diameters during BEPR decreased below FRC values whereas thoracic cephalocaudal dimension greatly increased. During PPAV, all thoracic dimensions increased. Despite these dissimilar regional chest movements, VA, VD, and VD/VT ratio were comparable between PPAV and BEPR under conditions of matched tidal volume and respiratory frequency. Stability in upper lobe ventilation during BEPR was maintained by caudal displacement despite the compression of the rib cage, as documented by tantalum bronchography. Lobar-interdependence appears to be the mechanism transmitting negative pleural pressure developed by the diaphragm to the upper lobes via lower and middle lobe inflation.

Animals↗
PubMed1975

Urinary metabolites from gamma- and beta-BHC in the mouse: chlorophenol conjugates.

Metabolites produced from gamma- and beta-BHC in the mouse urine were purified and characterized. Most metabolites from both isomers were not extractable by non-polar organic solvents and were conjugates such as sulfates and glucuronides. After hydrolysis with an appropriate enzyme, the conjugates gave chlorophenols, among which 2,4,6-trichlorophenol existed most abundantly (total, about 25% in the urine metabolites). 2,4-Dichlorophenol also constituted a significant portion.

Animals↗