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Biomedical subjects

N Krueger

Publications and source records attributed to N Krueger.

10 recordsLinked to original sources
PubMed1995

Bronchopneumonia in mice caused by Pasteurella haemolytica A2 after predisposition by ovine Bordetella parapertussis.

Initial intranasal inoculation of four to eight-week-old Swiss White mice with 7.5 x 10(6) colony forming units (cfu) of ovine B. parapertussis followed 30 min, three or five days, by intranasal inoculation with 1.4 x 10(5) cfu of Pasteurella haemolytica A2 resulted in a more severe infection pattern than when either agent was administered alone. Histopathological examination showed that inoculation with B. parapertussis alone caused a bronchopneumonia the severity of which was dependant upon the infecting dose. Bacteria were recovered up to 10 days after inoculation. P. haemolytica alone had no apparant pathogenic effect and was cleared from the lungs within 24 h. When both agents were given in combination the lesions were most severe when P. haemolytica was administered three days after B. parapertussis infection. These findings suggest that B. parapertussis predisposes mice to subsequent infection with P. haemolytica and that the timing of the P. haemolytica administration influences the severity of the lung lesions.

Animals↗
PubMed1995

Predisposition of specific pathogen-free lambs to Pasteurella haemolytica pneumonia by Bordetella parapertussis infection.

Three groups of specific pathogen-free (SPF) lambs were inoculated intratracheally with an ovine isolate of Bordetella parapertussis (5.5 x 10(9) colony-forming units) or with B. parapertussis followed 2 or 5 days later with Pasteurella haemolytica serotype A2 (120-180 million colony-forming units). When P. haemolytica A2 was administered 2 days after infection with B. parapertussis all lambs became febrile for at least 72 h. At necropsy their lungs were discoloured, congested and showed large areas of collapse and consolidation which, in one case, covered the entire lung. Histopathological examination confirmed that the combined infection produced a severe acute bronchopneumonia in four of seven lambs. B. parapertussis and P. haemolytica were recovered from all of the lambs in this group. Seven lambs challenged with P. haemolytica 5 days after B. parapertussis and six lambs infected with B. parapertussis alone showed no clinical signs of disease other than mild pyrexia and only mild histopathological changes. B. parapertussis, but not P. haemolytica, was recovered from these lambs. The findings indicated that B. parapertussis predisposed the SPF lambs to P. haemolytica pneumonia. This effect appeared to be dependent upon the time interval between the administration of the two agents.

Animals↗
PubMed1995

Phenotypic characterization of the cells of the inflammatory response in ovine encephalitic listeriosis.

The brainstem (pons cerebri and medulla oblongata) of 22 sheep aged between 6 months and 3 years which had developed clinical signs of central nervous system dysfunction were examined. Histopathological changes characterized by microabscesses, focal gliosis and perivascular cuffing compatible with natural infection with Listeria monocytogenes were present. The brains were examined by lectin histochemistry and immunohistochemistry with markers for T lymphocytes (CD4+ and CD8+ subsets), B lymphocytes, mononuclear phagocytes (including macrophages, ramified microglia, activated microglia and amoeboid microglia), astroglia and L. monocytogenes. These methods allowed semiquantitative analyses of the frequency of the different cell types in the brain lesions. The distribution of listerial antigen in the lesions was variable but always sparse. Mononuclear phagocytes and neutrophils appeared to be the most numerous inflammatory cells in the affected areas of the brainstem. T lymphocytes (CD8+ and CD4+ subsets) and B lymphocytes also played a part in the inflammatory process, in addition to activated astrocytes.

Abscess↗
PubMed1994

Detection of louping ill virus in formalin-fixed, paraffin wax-embedded tissues of mice, sheep and a pig by the avidin-biotin-complex immunoperoxidase technique.

An immunohistochemical method for the detection of louping ill virus antigen in formalin-fixed, paraffin wax-embedded tissues by an avidin-biotin-complex (ABC) immunoperoxidase technique was established. The tissues examined were from the brains of 10 mice, five sheep and one pig. The mice were experimentally infected with louping ill virus whereas the sheep and the pig were field cases of louping ill confirmed by clinical examination, and by histological and serological methods.

Animals↗
PubMed1988

Cost and quality effects of outpatient cataract removal.

In March 1985, a Health Care Financing Administration regulation went into effect requiring that cataract removal without exceptional circumstances be done in outpatient settings. In this paper, we study implications of that mandate by comparing the cost and quality outcomes of cataract removal in outpatient and inpatient settings both before and after the regulation went into effect. After controlling for population and physician differences for both study periods, we found by chi square significantly fewer infections, suture adjustments, and pain requiring medication among outpatients than inpatients. Log-linear regression, however, found that the only significant predictor was inpatient or outpatient, and only for infection (p = .02), with an odds ratio of 7.55 (95% confidence interval; .92-61.60). We also found lower Medicare payments for outpatients in both study periods. For the preregulation study groups, inpatient care was 34.8% more costly than outpatient care; the cost differential dropped to 32.5% for the postregulation study groups.

Aged↗
PubMedDate not supplied

Managed care.

Health-care reform has become a national mandate. Managed care and managed competition have been reinforced by the Clinton health-care reform plan. What type of health-care delivery system can laboratory managers expect to be part of in the near future? We asked laboratory managers who are currently involved in those types of systems. The result is a two-part As We See It. In part one, we will cover physician partnerships and the system laboratory as we ask: What can the laboratory community expect from a managed-care environment?

Administrative Personnel↗
PubMedDate not supplied

Managed care.

Many say 1994 will be the year for health-care reform, but those in the thick of the current health delivery system know that 1993 has already brought many sweeping reforms. These reforms will be studied, refined, and tailored for the national plan. What types of reforms are laboratorians seeing now? What kinds of changes can we expect in the upcoming months and years? We asked laboratory managers those very questions. The result has been a two-part As We See It. In the last CLMR, we covered physician partnerships and the system laboratory. In this issue, we will cover alliances with hospitals and laboratory cost reductions as we ask: What can the laboratory community expect from a managed-care environment.

Contract Services↗