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Biomedical subjects

N Krivoy

Publications and source records attributed to N Krivoy.

At least 37 records · Page 2Linked to original sources
PubMed1995

[Cholestyramine for digoxin intoxication].

Cholestyramine, a bile acid sequestering resin, has been reported to bind digitalis in vitro. We gave 4 g every 6 hours to 3 patients with non-life threatening digoxin intoxication. In all 3 serum digoxin concentrations and digoxin half-life decreased: from 50 to 32 hours, from 50 to 10 hours and from 46 to 16 hours in the 3 cases, respectively. Cholestyramine is potentially useful and safe adjunct medication for non-life threatening digoxin intoxication.

Aged↗
PubMed1995

[Bronchocentric granulomatosis].

We describe a 42-year-old woman with 3 solitary lung lesions in whom the diagnosis of bronchocentric granulomatosis was made by open lung biopsy. She presented with pleuritic chest pain, dry cough and fever. X-ray revealed 2 solitary nodules in the lower lobe of the right lung, and another in the lingula. The pathologic findings included necrotizing granulomatous inflammation, mainly in the bronchi and bronchioles. The lesions of the right lung were resected, resulting in complete recovery from symptoms, while the nodule in the lingula resolved spontaneously.

Adult↗
PubMed1992

Application of cross-flow filtration to the purification of biologically active peptides in human plasma after incubation with a protease-rich extract.

The aim of this study was to find an experimental procedure to purify biologically active peptides from a complex biological matrix (plasma), which was incubated with a protease-rich extract (submandibular gland extract). Special interest was focused on the practicability of cross-flow filtration for this purpose. Therefore, peptides in the incubation mixture were purified with a combination of high-performance liquid chromatographic steps. Purification of biologically active peptides was monitored by a sensitive bioassay and by laser desorption/ionization mass spectrometry. This permitted not only purity control at each purification step but also identification of one of the peptides with vasoconstrictor properties as angiotensin II. This result demonstrates the practicability of cross-flow filtration for extracting enzymatic reaction products from complex substrate-enzyme mixtures during the incubation.

Animals↗
PubMed1992

Generation of angiotensin II from human plasma by tissue kallikrein.

1. Human plasma was incubated with tissue kallikrein from porcine pancreas, dialysed to obtain a fraction with a molecular mass < 10 kDa and further purified by reverse-phase chromatography. 2. Vasopressor activity in the fractions obtained was tested in the isolated perfused rat kidney. 3. In one fraction a strong vasopressor action was found, which was blocked by saralasin and by an angiotensin II antibody. 4. Aprotinin inhibited the formation of vasopressor substances by tissue kallikrein. 5. U.v.-laser desorption/ionization mass spectrometry revealed a molecular mass of 1046 Da in the purified active fraction. 6. It is concluded that tissue kallikrein forms not only kinins, but also angiotensin II, from human plasma under physiological conditions.

Angiotensin II↗
PubMed1992

Endogenous digoxin-like immunoreactivity measured in seminal fluid from a normal male population.

Endogenous digoxin-like immunoreactivity (EDLI) has been detected in different biological fluids and in several pathophysiological conditions. In this study, using radioimmunoassay we reported for the first time the existence of bound and unbound EDLI in normal seminal fluid. The unusual finding was the detection of unbound EDLI in the seminal fluid, while this reactivity was undetected in plasma. Two main hypotheses are presented: (1) local secretion of unbound EDLI and/or (2) passive diffusion from plasma to the seminal fluid of unbound EDLI and subsequent local concentration.

Adult↗
PubMed1992

Peripartum changes in free and protein-bound digoxinlike immunoreactive factor.

The appearance of free digoxinlike immunoreactive factor in pregnancy and its rapid disappearance after delivery is well documented. The protein-bound fraction, a common component of plasma, does not change during pregnancy. We investigated the peripartum changes in both free and protein-bound fractions of digoxinlike immunoreactive factor and observed different peripartum patterns of those fractions. While the disappearance of the free fraction after delivery was reconfirmed, the protein-bound fraction exhibited a biphasic pattern: a rapid decrease after delivery with a slow increase to predelivery levels seven weeks later. The disappearance of free digoxinlike immunoreactive factor after delivery may reflect the elimination of the fetal source. The changes in the protein-bound fraction imply that the fraction may also vary in certain clinical situations and may be produced in response to certain homeostatic changes and suggest a possible interaction between fetal and maternal systems that produce digoxinlike immunoreactive factor during pregnancy.

Blood Proteins↗
PubMed1991

[Transient liver damage due to prajmalium bitartrate].

3 patients developed transient cholestatic jaundice after administration of prajmalium bitartrate, a class I antiarrhythmic drug. The leukocyte inhibition tests showed 4%, 12% and 15% inhibition, respectively, while eosinophilia was seen in all 3, supporting the assumption that the transient hepatic damage was due to drug exposure. Discontinuing the drug resulted in improvement in the clinical and biochemical findings.

Adult↗
PubMed1991

Endogenous digoxin-like immunoreactivity in follicular fluid and in vitro fertilization.

Plasma digoxin-like immunoreactive factor (DLIF) has been detected in various pathophysiological conditions associated with volume expansion. In this study, using radioimmunoassay, we confirmed the existence of high levels of DLIF in the stimulated follicular fluid, a rapidly volume-expanding biological model. The concentration of the various fractions of DLIF in follicular fluid was 2-9 times higher than in plasma, suggesting local concentration or production. No difference in concentration was observed between follicles containing fertilized oocytes and follicles with unfertilized oocytes. The role of DLIF in follicular homeostasis remains to be further investigated.

Blood Proteins↗
PubMed1991

Native valve Staphylococcus epidermidis endocarditis: report of seven cases and review of the literature.

This report describes seven patients from three university hospitals whose native valve infective endocarditis was caused by Staphylococcus epidermidis. The literature on endocarditis caused by S. epidermidis is also reviewed and the clinical features of patients with native valve endocarditis due to this organism are compared with those of patients from a general series of infective endocarditis cases. Compared with infective endocarditis caused by other organisms, S. epidermidis endocarditis tends to occur more frequently in male patients. Patients with S. epidermidis endocarditis exhibit fewer embolic complications and skin manifestations. The frequency of congestive heart failure is lower in this group. The relative indolent course and apparent rarity of native valve S. epidermidis endocarditis necessitate a high index of suspicion for early diagnosis.

Aged↗
PubMed1990

Total digoxin-like immunoreactive factor(s) in healthy population, uncomplicated term pregnancies and neonates.

Free digoxin-like immunoreactive factor(s) (DLIF) which may have a homeostatic role, as documented in different physiological conditions, but is generally undetectable in plasma from normal population. Total digoxin-like immunoreactive factor(s) (protein bound and free) can be estimated after plasma is heated. In this study, total digoxin-like immunoreactive factor(s) as measured in plasma in a well defined control population and compared to healthy term pregnant women and neonates, categories known to be associated with increased free digoxin-like immunoreactive factor(s) concentrations. The mean level of this factor(s) in the control group was 706 +/- 129 pg digoxin equivalent/ml (pg/ml) and was unaffected by age and sex. Significantly increased levels of total digoxin-like immunoreactive factor(s) were found in pregnant women and neonates (928 +/- 127 and 1242 +/- 367 pg/ml, respectively). We conclude that levels of total digoxin-like immunoreactive factor(s) are increased in term pregnancies and neonates, similarly to its free form. However total digoxin-like immunoreactive factor(s) is detected in the normal population as a plasma component, contrary to its free form, which is generally undetectable.

Adult↗