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Biomedical subjects

N Kitamura

Publications and source records attributed to N Kitamura.

At least 433 records · Page 24Linked to original sources
PubMed1987

Immunocytochemical demonstration of gastric endocrine cells in the stomach gland patch of the koala, Phascolarctos cinerus.

The relative frequency and topographical distribution of endocrine cells containing gut hormones were studied by immunocytochemistry in the stomach gland patch of the koala. Numerous somatostatin- and a moderate number of bovine pancreatic polypeptide-immunoreactive cells were found primarily in the lower two-thirds of the oxyntic glands. 5-hydroxytryptamine-immunoreactive cells were few in number and scattered throughout these glands. Glucagon-immunoreactive cells were observed only rarely and then were confined to the basal region of the oxyntic glands.

Animals↗
PubMed1987

Distribution of methionine-enkephalin-Arg6-Gly7-Leu8-immunoreactive nerves in the forestomach of cattle.

The distribution of methionine-enkephalin-Arg6-Gly7-Leu8-immunoreactive nerves in the forestomach of calves and cows was studied, using immunohistochemical methods. Methionine-enkephalin-Arg6-Gly7-Leu8-immunoreactive nerves were found in all regions of the bovine forestomach. Cell bodies were found mainly in myenteric ganglia, and nerve fibers were found principally in muscular layers. Nerve fibers and ganglia were concentrated in the lips of the reticular groove, but were least numerous in ruminal and omasal walls. The reduced number of immunoreactive nerves in cows, compared with the number of those in calves, was striking, especially in the lips of the reticular groove and the omasal leaves. Results may indicate that the importance of methionine-enkephalin-Arg6-Gly7-Leu8-containing nerves in the physiologic regulation of the forestomach of the calf is different from that of the cow.

Aging↗
PubMed1986

Immunohistochemical study on the distribution of neuron-specific enolase-and peptide-containing nerves in the reticulorumen and the reticular groove of cattle.

The distribution of nerves containing immunoreactivity for substance P (SP), vasoactive intestinal polypeptide (VIP), leucine-enkephalin (LENK), gastrin-releasing polypeptide (GRP), and somatostatin (SOM) was studied in the reticulorumen and the reticular groove of the calf and cow, use immunohistochemistry. Neuron-specific enolase (NSE) antiserum was used to determine the general distribution of nerves, which was then compared to distribution of immunoreactive (IR) nerves stained for five kinds of peptides. Nerve fibers and cell bodies were observed in all segments examined; however, both elements showed considerable variation in different segments and age groups. The densities of nerve fibers and ganglia were most concentrated in the reticular groove and least numerous in the ruminal wall. VIP-, SP-, and LENK-IR nerves were detected in all segments, whereas GRP- and SOM-IR nerves were detected only in the reticular groove. NSE-IR nerves were moderately abundant in the reticulorumen, but peptide-IR nerves composed only a minor population of nerves with VIP-IR nerves being most abundant. SP-IR nerves were less abundant and LENK-IR nerves were rarely observed. Abundant nerves were detected in the lips of the reticular groove and of these, SP-IR nerves were most abundant, and in order of decreasing abundance, VIP-, LENK-, SOM-, and GRP-IR nerves were observed also. Immunoreactive nerves were more abundant in the calf than in the cow. The reduced abundance of immunoreactive nerves in the cow as compared to calf was striking, particularly with regard to the SP-IR nerves located in the lips of the reticular groove.

Animals↗
PubMed1986

Human calcitonin gene-related peptide possesses weak inhibitory potency of bone resorption in vitro.

Synthetic human calcitonin gene-related peptide (CGRP) was examined for the action on the bone in vitro. Human CGRP inhibited bone resorption stimulated by both human parathyroid hormone and basal. The mode of inhibitory action of human CGRP seemed to be similar to that of calcitonin and the relative potency of human CGRP to inhibit bone resorption is one five-hundredth of that of human calcitonin. Thus, a novel pharmacological action of CGRP was demonstrated.

Animals↗
PubMed1986

Isolation and sequencing of a genomic clone for mouse brain specific small RNA.

We isolated a mouse genomic clone that hybridized with small RNA present in the cytoplasm of the brain. The RNA was about 150 nucleotides long. This RNA seemed to be specific to the brain, since it was not found in the liver or kidney. The clone DNA contained a sequence homologous to 82-nucleotide "identifier" core sequence of cDNA clones of rat. The sequence contained a split promoter for RNA polymerase III and was flanked by a 12-nucleotide direct repeat (ATAAATAATTTA).

Animals↗
PubMed1986

Sclerotic bone metastasis: radiologic-pathologic correlation.

Radiographic appearances were correlated with histologic findings in sclerotic bone metastases. For this purpose, undecalcified ground sections were made from lumbar vertebrae obtained at 12 autopsies. The primary tumor sites were the prostate gland in five cases, the breast in three, the lung in two, the stomach in one, and the urinary bladder in one. After Villanueva bone staining and methyl methacrylate embedding, 1-mm-thick ground sections were made for low-kilovoltage contact radiography. In sclerotic bone metastases, new bone is deposited on preexisting trabeculae and in the intertrabecular spaces, forming lacy networks. The radiographic indications of these histologic changes are trabecular thickening, marginal obscurity (the "silhouette" sign), and homogeneous dense sclerotic change. The silhouette sign in bone is a new concept, one that is helpful when the changes in density are not reliable.

Adult↗
PubMed1986

Immunohistochemical study of gastroenteropancreatic endocrine cells of the herbivorous Japanese field vole, Microtus montebelli.

The gastroenteropancreatic (GEP) endocrine cells of the Japanese field vole were studied immunohistochemically. Somatostatin-, 5-hydroxytryptamine-, glicentin-, glucagon-, bovine pancreatic polypeptide-, gastrin-, gastric inhibitory polypeptide-, cholecystokinin-, substance P-, secretin-, neurotensin- and insulin-immunoreactive cells were revealed. The characteristic findings of the regional distribution and relative frequency of these immunoreactive cells in the GEP system of the vole were as follows. Somatostatin-immunoreactive cells were more numerous in the oxyntic glands than in the pyloric glands. Some somatostatin-immunoreactive cells were found in small clusters in the oxyntic glands. Gastrin-immunoreactive cells were detected not only in the pyloric glands and small intestine but also in the caecum and spiral colon. Gastric inhibitory polypeptide-immunoreactive cells were also detected in the pyloric glands and no motilin-immunoreactive cell was found in the gastroenteropancreatic system.

Animals↗