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Biomedical subjects

N King

Publications and source records attributed to N King.

At least 127 records · Page 7Linked to original sources
PubMed1984

Use of vitamin supplements for burned patients: a national survey.

Vitamin losses and requirements of critically ill and burned patients remain undefined in the current research and clinical literature. A survey was conducted to determine the vitamin supplementations routinely prescribed for burned patients, dosages, and the criteria for administration. A questionnaire was sent to 271 health care providers (dietitians and physicians) who work in burn care facilities in the United States. Forty-seven percent of the questionnaires were completed and returned. They showed that 87% of the respondents routinely prescribed vitamin supplementation. Of those, 97% used some kind of multivitamin preparation. Fifty-eight percent of the multivitamin dosages exceeded 100% of the Recommended Dietary Allowances. Several respondents indicated that extra vitamins were given in addition to the multivitamin preparations. Most facilities used more than one specific criterion for prescribing vitamins when supplementation was not routine. Burn size, nutritional status prior to admission, and poor dietary intake were the criteria most commonly identified. Further research is needed to provide guidelines for the kind and amount of vitamin supplements needed for burned patients.

Burns↗
PubMed1979

Pet health insurances and contract services: the potential for practice involvement.

Pet medical insurance has appreciable advantages and the existing policies appear to provide a beneficial service. But administrative costs of the small premiums involved make it of doubtful commercial attraction or benefit to the client. However, practice contract service schemes reduce administrative costs and can provide many of the advantages of insurance. Such a scheme is described. A coordinating agency outside the practice to produce promotional material, contracts, formulae for setting premiums and exclusions and to act as an arbitration agency for disputes could be established given the demand.

Animals↗
PubMed1978

Endogenous New World primate type C viruses isolated from owl monkey (Aotus trivirgatus) kidney cell line.

A type C virus (OMC-1) detected in a culture of owl monkey kidney cells resembled typical type C viruses morphologically, but was slightly larger than previously characterized mammalian type C viruses. OMC-1 can be transmitted to bat lung cells and cat embryo fibroblasts. The virions band at a density of 1.16 g/ml in isopycnic sucrose density gradients and contain reverse transcriptase and a 60-65S RNA genome composed of approximately 32S subunits. The reverse transcriptase is immunologically and biochemically distinct from the polymerases of othe retroviruses. Radioimmunoassays directed to the interspecies antigenic determinants of the major structure proteins of other type C viruses do not detect a related antigen in OMC-1. Nucleic acid hybridization experiments using labeled viral genomic RNA or proviral cDNA transcripts to normal cellular DNA of different species show that OMC-1 is an endogenous virus with multiple virogene copies (20-50 per haploid genome) present in normal owl monkey cells and is distinct from previously isolated type C and D viruses. Sequences related to the OMC-1 genome can be detected in other New World monkeys. Thus, similar to the Old World primates (e.g., baboons as a prototype), the New World monkeys contain endogenous type C viral genes that appear to have been transmitted in the primate germ line.

Animals↗
PubMed1968

Brush border particulates of renal tissue.

Particulates containing a large part of the alkaline phosphatase activity of renal tissue were separated from homogenates and from ribosomal preparations by zonal centrifugation. The particles had a high content of phospholipid and cholesterol that was not removed by treatment with I percent deoxycholate. Enzymatic activities concentrated with the particles were the alkaline phosphatase, a peptidase resistant to proteolysis, glucose-6-phosphatase, inorganic pyrophos-phatase, and adenosine triphosphatase. The particles accumulated leucine with no stimulation from soluble factors and with inhibition by other amino acids; the accumulation was stimulated by adenosine triphosphate and was not inhibited by puromycin. The particles appear to be derived from the membranes of the brush borders of tubular cells.

Adenosine Triphosphatases↗
PubMedDate not supplied

Patients as peer preceptors for orthopedic oncology rehabilitation patients.

This article describes a peer preceptor program that trains patients who have experienced limb preservation surgeries and managed long-term rehabilitative therapy to support and teach newly diagnosed patients through their rehabilitation. This preceptor program was designed to complement the professional counseling and support that rehabilitation staff provide to patients and their families. Training includes communication techniques, practice sessions using videotaped scenes of patients and their families, as well as role-playing. Experienced patients who became preceptors as a result of this program were orthopedic oncology patients who had completed limb preservation surgery, chemotherapy, grafting, and physical therapies. After their training, preceptors exhibited improved communication skills in terms of empathy, listening, and voice expressions. Initiatives designed to make the preceptor program self-sustaining by having staff nurses manage the training still have to be tested.

Extremities↗
PubMedDate not supplied

Experimental evidence of cerebral injury from profound hypothermia during cardiopulmonary bypass.

Choreoathetosis, seizures, and impaired mental development continue to occur in children undergoing cardiopulmonary bypass (CPB) and profound hypothermia with or without circulatory arrest. Although there is some evidence that the hypothermia itself may be causing these neurologic problems, skepticism remains because of lack of evidence from experimental studies simulating the clinical setting. In this experimental study, we examined the effect of profound and moderate hypothermia on the brain while maintaining normal flow rates during CPB. Ten adult mongrel dogs equally divided into two groups were anesthetized and subjected to CPB and varying levels of hypothermia (group 1, < or = 15 degreesC; group 2, < or = 2 degreesC). Both groups were kept at the desired temperature for 1 hour prior to rewarming and discontinuation of CPB. The dogs were euthanized 4-6 weeks later and neuropathologic studies were performed. The mean CPB flow rates during cooling and at the desired rectal temperature were comparable in both groups: group 1, 108 +/- 10 ml/kg/min versus 106 +/- 7 ml/kg/min in group 2 (p = NS) and 95 +/- 12 ml/kg/min in group 1 versus 101 +/- 5 ml/kg/min in group 2 (p = NS). Because of the difference in temperature between the two groups, the mean cooling time (onset of CPB to desired rectal temperature) was longer in group 1 (70 +/- 14 minutes) than in group 2 (28 +/- 11 minutes, p = 0.007). Hence, the total mean CPB time was also longer in group 1 (198 +/- 25 minutes) than in group 2 (143 +/- 13 minutes, p = 0.002). The lowest mean blood and rectal temperature achieved in group 1 were 11 +/- .9 degreesC and 12 +/- 1 degreesC versus 29 +/- .4 degreesC (p < 0.001) and 30 +/- .6 degreesC (p = 0.001), respectively, in group 2 (p = 0.001). Neuronal loss and degeneration was noted in all dogs in group 1 ranging from 2 to 8 cells per 1000 cells counted compared to none in group 2 (p = 0.05). These lesions occurred in both the basal ganglia and the cortex, although they were more marked in the caudate when compared to the cortex and cerebellum. Both in the cortex and in the caudate, neuronal loss was more marked around the capillaries. We conclude that the use of profound hypothermia of < or =15 degreesC and maintenance of normal flow rates during cooling at this temperature for 1 hour produces neuronal loss and degeneration in the brain. These lesions being more marked around capillaries points to the vulnerability of the neurons, probably because of their high lipid content to injury from the cold perfusate.

Animals↗
PubMedDate not supplied

A new method of quantifying glutathione levels in freshly isolated single superfused rat cardiomyocytes.

INTRODUCTION: Glutathione (GSH) is an important antioxidant in the heart whose content changes during cardiac insults. However, there are currently no methods for continuously monitoring free cytoplasmic GSH levels in single isolated and superfused cardiomyocytes exposed to normal and pathological conditions. METHODS: GSH was measured using CellTracker Blue CMAC (Molecular Probes), a member of a new family of thiol-sensitive dyes. The fluorescence of 5 microM CellTracker Blue CMAC was measured in various solutions containing glutathione-S-transferase and in freshly isolated single superfused cardiomyocytes using an inverted fluorescence microscope. The cardiomyocytes were isolated by standard procedures and loaded with either CellTracker Blue CMAC or monochlorobimane by 15 min of shaking incubation in the dark at room temperature followed by centrifugation with resuspension of the cells in dye-free media. Cell volume was calculated from the 3H2O and [14C]sucrose space. RESULTS: CellTracker Blue CMAC fluorescence was linearly proportional to 0-100 microM GSH, as described by the equation: Y = 182.2 (X) + 681.6 (r2 = .99, P < .001). Fluorescence was not affected by changing the glutathione-S-transferase level, the calcium concentration, or the pH, neither was the fluorescence quenched by H2O2 or cyanide. Exposure of freshly isolated single superfused cardiomyocytes to oxidative stress in the presence of 0-1 mM H2O2 caused a progressive decrease in cellular GSH. In contrast, brief exposure to metabolic inhibition in the presence of 2.5 mM NaCN evoked a significant increase in cardiomyocyte GSH followed by a return to control levels during washoff. In comparison to monochlorobimane, cells loaded with CellTracker Blue CMAC gave a stronger signal with better cellular retention of the probe. DISCUSSION: These results suggest that CellTracker Blue CMAC fluorescence will be a good tool for measuring GSH in freshly isolated single superfused cardiomyocytes because it shows the expected changes to oxidative stress and metabolic inhibition, and is reversible.

Animals↗
PubMedDate not supplied

Estimation of the lumbar curve magnitude with correction of the right thoracic curve in idiopathic scoliosis.

A simple formula was proposed to estimate the magnitude of the postoperative uninstrumented lumbar curve with correction of the right thoracic curve in idiopathic scoliosis. This formula is as follows: PLC < or = LC - 0.5(TC - BTC) (PLC, predicted postoperative standing lumbar Cobb angle; LC, preoperative standing lumbar Cobb angle; TC, preoperative standing thoracic Cobb angle; BTC, preoperative supine lateral bending thoracic Cobb angle). Sixty-five patients' preoperative and postoperative radiographic measurements were taken, and of these 45 had measurements taken after > or = 12 months of follow-up. Multiple regression (R) value for the proposed formula postoperatively was 0.8048 and at > or = 1 year follow-up was 0.6869.

Adolescent↗