Biomedical subjects

N K Isaev

Publications and source records attributed to N K Isaev.

21 records · Page 2Linked to original sources

PubMed1992

[Effects of nerve growth factor on the development of the dendritic system of cholinergic neurons in dissociated culture of the rat septum].

Neurons dissociated from septal area of fetal (E18-19) rat brain were grown 14-days in culture. Cholinergic neurons were identified by cytochemical demonstration of acetyl cholinesterase. It was shown that the nerve growth factor added to the culture medium (50 u/ml) has increased the size of cell body of AchE-positive neurons, mean total length and arborization of dendrites and also the dendritic tree area.

Animals↗

PubMed1991

[The effect of nerve growth factor on cholinergic neurons in dissociated cultures of the septum pellucidum].

Neurons dissociated from septal area of fetal (E 18-19) rat brain were grown for 7 days in culture. Cholinergic neurons were identified by cytochemical demonstration of acetyl cholinesterase. Addition of the nerve growth factor to the culture medium (200 u/ml) increases the number of AChE-positive neurons, the size of the cell body and activity of AChE in these neurons.

Acetylcholinesterase↗

PubMed1990

[The use of a polyethyleneimine substrate for culturing dissociated cells of the central nervous system].

A new method of preparation of polyethylenimin substrate for cultivation of dissociated cells from different parts of the central nervous system is presented. The alcohol solution of polyethylenimin used for this substrate is easily prepared, needs no sterilization and can be stored unlimitedly. The substrate provides a good attachment, growth and differentiation of neurons and glial cells and possesses all the advantages of more expensive polyaminoacid substrates (polylysine, polyornithine). Our substrate is highly adhesive, displays good optical properties and no artefacts after histological and histochemical treatment.

Animals↗