The synthesis and purification of new fluorescently labeled derivatives of GDP and ATP are described. The fluorescent groups are coupled initially through amine-containing linker arms to periodate-oxidized nucleotides. Reduction of the initial product yields primarily a six-membered morpholine-like ring. Fluorescein-labeled GDP, rhodamine-labeled GDP, and fluorescein-labeled ATP were characterized by absorbance spectroscopy and TLC. NMR and FAB-MS studies were carried out on a single nucleotide derivative formed by reacting periodate-oxidized guanosine and benzylamine with subsequent reduction to establish the modification to the ribose moiety. The synthesis of the guanosine-benzylamine conjugate led to a mixture of products that were separated. The predominant product (70%) resulted in conversion of the ribose moiety to a six-membered morpholine-like ring having no hydroxyl group, and the minor product (30%) resulted in an open ring structure having one hydroxyl. When NaCNBH3 was used as the sole reductant, only the product with the morpholine-like ring was formed. These probes were prepared for use in solution studies of the interactions of eukaryotic initiation factor-2 with other components of mammalian protein synthesis initiation.

Adenosine Triphosphate↗

PubMedDate not supplied

Strabismus fixus convergens secondary to amyloidosis.

Strabismus fixus convergens is a condition in which both eyes become fixed in extreme adduction. It can be congenital or acquired. We report a case of strabismus fixus convergens in a high myope with primary amyloidosis localized to the lateral rectus muscle. To our knowledge, this is the first report of a case of strabismus fixus convergens secondary to amyloidosis.

Amyloidosis↗

PubMedDate not supplied

Myopic strabismus fixus convergens.

A case report of myopic myositis, a rare entity, leading to strabismus fixus convergens, occurring in a high myope at the age of 46 years is presented. The possible differential diagnosis along with the aetiopathogenesis is discussed.

Convergence, Ocular↗

PubMedDate not supplied

Ectopia lentis et pupillae.

A rare case of congenital bilateral ectopia lentis et pupillae (ELEP) is reported in a 32 year old female presenting with complaints of diminution of vision in both eyes. Associations of optic nerve hypoplasia and myelinated nerve fibers are being reported for the first time in such a case. The aetiopathogenesis of ELEP is controversial. Combined neuroectodermal and mesodermal origin is being postulated for this syndrome on the basis of aforesaid abnormalities and presence of pupillary dilator muscle hypoplasia, "annular endotheliopathy", and persistent pupillary membrane in both eyes.

Adult↗

PubMedDate not supplied