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Biomedical subjects

N Ishiguro

Publications and source records attributed to N Ishiguro.

At least 163 records · Page 9Linked to original sources
PubMed1990

Meningo-encephalitis associated with HHV-6 related exanthem subitum.

We report a 10-month-old boy with acute meningo-encephalitis associated with exanthem subitum. It has recently been reported that human herpesvirus-6 is the causative agent of exanthem subitum, and to our knowledge our case is the first report of meningo-encephalitis associated with HHV-6 infection.

Exanthema Subitum↗
PubMed1990

[The analysis of peripheral blood lymphocyte subsets in patients with Epstein Barr virus induced hepatitis].

Both humoral and cell-mediated immunity to Epstein Barr virus related antigen have recently been reported and are thought to be important for the generation of Epstein Barr virus induced human diseases. For this reason, to study immunological features in Epstein Barr virus induced hepatitis (EBH), we studied the ratio of various immunocompetent cells in peripheral blood and compared them to that of healthy control and acute hepatitis (disease control). We found that a significant elevation of CD8+ CD11- cells existed in EBH patients (48.9 +/- 18.3% in EBH, 17.0 +/- 6.9% in AH and 13.6 +/- 7.0% in healthy control) and atypical lymphocytes which are frequently observed in EBH belong to CD8+ CD11- cells. Regarding the study of surface expression of activated T cell markers, we found that these CD8+ cells also expressed HLA-DR antigen on their cell surface. Furthermore, the percentage of CD8+ CD11- cells returned to normal level with the recovery of their liver function. From these results, we can assume that activated CD8+ CD11- cells may have an important roles in generation of EBH.

Adult↗
PubMed1989

Characterization of replicative form DNA of the autonomous parvovirus mink enteritis virus.

Characterization of replicative form (RF) DNA of mink enteritis virus (MEV) was carried out. Most of the RF DNA were bound to terminal protein but some were free from the protein. The protein-free RF DNA increased about 7 times from 30 to 50 hr post-infection, while the DNA with protein increased less. The molecules of the replicative intermediate which were partially single-stranded DNA and bound to terminal protein were present. Two terminal conformations, "extended" and "turnaround," were observed in both ends of both terminal protein-bound and protein-free RF DNA. The 5' end labeling revealed that 5' ends of protein-free RF DNA were not blocked to phosphorylation by an amino acid or an oligopeptide which attaches to 5' ends of proteolytically deproteinized RF DNA. Restriction analysis of incomplete RF DNA which was partially double-stranded DNA showed that extended conformation was dominant in such incomplete RF molecules.

Cell Line↗
PubMed1989

Nucleotide sequence of a citrate utilization gene from Citrobacter amalonaticus.

The chromosomal DNA fragment (BamHI-BglII fragment of 2,972 base pairs) conferring citrate utilization in Citrobacter amalonaticus ATCC 25405 was cloned and sequenced. Two genes (citA and citB) identified in the Cit+ determinant were found in a BamHI-BglII fragment from C. amalonaticus. Southern DNA-DNA hybridization experiments and the construction of Cit- mutants of C. amalonaticus showed that C. amalonaticus has a single copy of the cit gene on the chromosome.

Base Sequence↗
PubMed1989

[Typical and atypical cases of myrmecia--the differences in the nature of the inclusion bodies and the associated human papillomavirus types].

A case of typical myrmecia and a case of clinically atypical, cutaneous horn-like myrmecia were studied histologically and molecular biologically. The former had eosinophilic, granular shaped inclusion bodies within the cytoplasm, as seen in other typical reported cases. In contrast, the latter was found to have more homogeneous, palely stained inclusion bodies. The analysis of human papillomavirus (HPV) DNAs detected in them clarified the association of HPV type 1 with the former and a different HPV type in the latter. These results confirmed that not only HPV type 1 but also other HPV types could produce myrmecia (inclusion wart) and suggested that the atypical case reported here might be better called by another name than myrmecia.

Adult↗
PubMed1989

[Composite tissue allograft limb in rats immunosuppressed with cyclosporine A].

The purpose of this study was to examine the mechanism of rejection after limb allografts and the effect of Cyclosporine A (CYA) on this process. Male F344 rats were used as recipients, Lew and WKAH as donors. After transplantation, CYA (15mg/kg/day s.c.) was administered for 21 days. It was possible to achieve a long-term survival of allograft limbs with CYA, but the skin was soon rejected after the termination of CYA administration. Without immunosuppression skin was rapidly rejected. It is conceivable that this rejection process takes place both by direct destruction of epithelial cells, by non-helper T lymphocytes and through injury to the microvascular endothelium of dermis. The mechanism of the microvascular injury remains unknown. The skin of some of the animals enhanced with CYA was transformed, but immunohistologically represented no sign of classic rejection. After termination of CYA treatment, a degenerative change of skin was observed. It is considered that this change proceeds through direct destruction of epithelial cells by non-helper T lymphocytes.

Animals↗
PubMed1988

Promoters and transcription of the plasmid-mediated citrate-utilization system in Escherichia coli.

The nucleotide sequence of an 878-bp BamHI-BglII restriction endonuclease fragment from citrate utilization transposon Tn3411 was determined, and was compared with that from plasmid pMS185 [Sasatsu et al., J. Bacteriol. 164 (1985) 983-993]. A long open reading frame for a 379-amino acid (aa) polypeptide (citB) was found 5' to the citA gene (431-aa membrane protein) in Tn3411 as well as in pMS185. Promoter regions were identified by RNA polymerase filter-binding assays, S1 nuclease mapping and cit-lac fusion experiments. The results indicated that two genes (citA and citB) have separate promoters, and the location of the promoter for the citB gene in the Tn3411 nucleotide sequence was different from that in pMS185. The regulation of transcription of the two genes (citA and citB) was characterized by the use of cit-lacZ fusions. The level of the citB promoter activity was about five-fold higher than that of the citA gene promoter, and transcription from both was not induced by citrate. Synthesis of the mRNA for the citB gene (especially with the wild-type Cit+ determinant) was suppressed by citrate, accompanying growth suppression of Escherichia coli. The citB gene expressed in E. coli minicells produced a membrane-associated 37.5-kDa polypeptide.

Amino Acid Sequence↗
PubMed1988

Nucleotide sequence of insertion sequence IS3411, which flanks the citrate utilization determinant of transposon Tn3411.

The nucleotide sequences of insertion sequences IS3411L (left) and IS3411R (right), present as direct terminal repeats in the citrate utilization of citrate utilization transposon Tn3411, and of IS3411 (generated by intramolecular recombination between IS3411L and IS3411R) were determined. The three IS3411 elements (IS3411R, IS3411L, and IS3411) were 1,309 base pairs long and identical in DNA sequence. IS3411 had 27-base-pair terminal inverted repeats with three bases mismatched and one long open reading frame (240 amino acids) that was proposed to be a transposase. Three polypeptides of 29,000, 27,000, and about 10,000 molecular weight, determined by IS3411, were identified in minicells. Since Tn3411 generates a 3-base-pair repeat upon integration, the nucleotide sequences of IS3411 were compared with those of IS3.

Amino Acid Sequence↗
PubMed1986

Conservation of DNA sequences for plasmid-mediated citrate utilization within the enterobacteria.

Southern blot DNA-DNA hybridization experiments with a cloned Cit+ DNA fragment as a probe showed that the plasmid-mediated Cit+ determinants from four Cit plasmids (R726, pOH3001, pOH3035, and pOH30221) were all homologous. Sequences homologous to the plasmid-borne Cit+ gene were also found in total bacterial DNA isolated from Salmonella paratyphi B, Salmonella enteritidis, Salmonella typhimurium LT-2, Citrobacter freundii, ATCC 8090, Citrobacter amalonaticus ATCC 25405, Klebsiella pneumoniae I and IID 977, and Enterobacter aerogenes ATCC 13048. The DNA digest from C. amalonaticus ATCC 25405 contained a 1.4-kilobase BamHI-HincII DNA fragment that was strongly homologous with and identical in size to the plasmid Cit+ probe.

Base Sequence↗
PubMed1986

[Pharmacokinetic studies on aztreonam in late pregnancy in sheep and humans].

The transplacental passage of single intravenous doses of aztreonam (AZT), 1 g or 2 g, was examined in 7 sheep and 14 women in late pregnancy, respectively and the obtained data were analyzed by a two-compartment model. The obtained results were summarized as follows. After single 2 g intravenous doses were given to pregnant sheep, the mean peak level of AZT in maternal blood was 83.79 micrograms/ml and the half-life of the beta-phase was 1.525 hours. After single 1 g intravenous doses were administered to pregnant women, the mean peak level of AZT in blood was 102.62 micrograms/ml and the half-life of beta-phase was 2.128 hours. The peak levels in umbilical venous blood and amniotic fluid were 14.43 micrograms/ml and 11.86 micrograms/ml, respectively.

Amniotic Fluid↗
PubMed1985

Nucleotide sequence of the gene determining plasmid-mediated citrate utilization.

The citrate utilization determinant from transposon Tn3411 has been cloned and sequenced, and its polypeptide products have been characterized in minicell experiments. The nucleotide sequence was determined for a 2,047-base-pair BglII restriction endonuclease fragment that includes the citrate determinant. This region contains an open reading frame that would encode a 431-amino-acid very hydrophobic polypeptide and which is preceded by a reasonable ribosomal binding site. However, the single polypeptide found in minicell experiments had an apparent molecular weight of 35,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Amino Acid Sequence↗