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Biomedical subjects

N Inaba

Publications and source records attributed to N Inaba.

At least 109 records · Page 6Linked to original sources
PubMed1984

Coexistence of adenocarcinoma and choriocarcinoma in the sigmoid colon.

A rare case of a colon carcinoma that developed in a 50-year-old woman is documented. Histologically, the removed tumor was composed of two distinctive elements, i.e., well-differentiated papillary adenocarcinoma and choriocarcinoma. It was strongly suggested that the latter malignancy derived from the pre-existing colonic carcinoma through metaplastic proliferation. A very high human chorionic gonadotropin level was noted in the patient's serum obtained at the preoperative stage. Autopsy materials revealed that metastatic foci in the liver, lungs, and lymph nodes were exclusively composed of choriocarcinoma.

Adenocarcinoma, Papillary↗
PubMed1984

More confined indications for use of combined passive and active immunization for preventing perinatal development of hepatitis B virus carrier-state--based on the natural history of hepatitis B virus-vertical transmission.

To find more confined criteria for use of passive and/or active immunization for preventing perinatal development of hepatitis B virus (HBV) carrier-state than maternal HBe antigenemia, maternal HBsAg-titers (R-PHA) around delivery and infantile HBeAg-titers-(EIA) are discussed. No children whose maternal HBsAg-titers around delivery were lower than 3(6) developed carrier-state in spite of maternal HBe antigenemia. In addition, at age 2 months serum HBeAg-titers of 6 children who had acquired persistent HBsAb were lower than 25, while those of 5 children who had developed carrier-state were higher than 70. These findings may contribute to the establishment of more confined indications for the administration of HBIG and/or HB vaccine to the children born to HBeAg-positive carrier women, saving not only HBIG and HB vaccine but all accompanied efforts of both patients and medical staff as well.

Carrier State↗
PubMed1984

Effect of cytochalasin B on growth, Multinucleation and human chorionic gonadotropin secretion in a human choriocarcinoma cell line.

Treatment of human choriocarcinoma cells with cytochalasin B at the doses of inhibiting cell division but not inhibiting nuclear division led to in vitro formation of the multinucleated syncytiotrophoblast-like ( STL ) cells. A concomitant increase in human chorionic gonadotropin (hCG) secretion per cell was noted. Immunocytochemical staining demonstrated the predominant localization of hCG in the STL cells. These results indicate that multinucleation stimulates hCG synthesis and secretion in the choriocarcinoma cells.

Cell Division↗
PubMed1984

The immunocytochemical location of two membrane-associated placental tissue proteins in human and cynomolgus monkey placentae.

Using the PAP technique, the location of two new membrane-associated placental tissue proteins, MP1 and PP4 was studied in the placenta, its membranes, decidua and umbilical cord of human and cynomolgus monkeys. The results were the same throughout pregnancy. MP1 was located in the syncytiotrophoblast and trophoblastic cells of the reflected chorion. Other placental tissue components (cytotrophoblast, amnion, decidua, and umbilical cord) were negative. At present, MP1 appears to be specific for trophoblast. PP4 was located in the syncytiotrophoblast. Furthermore clear positive staining for PP4 was found in the villous cytotrophoblast, reflected and basal chorion, amnion, umbilical cord and decidua. In addition, PP4 was positive in some granulocyte-like blood cells in the intervillous space. Immunocytochemically, the most positive staining for both proteins was observed in the membrane of villous syncytiotrophoblastic cells. The findings in the placenta of cynomolgus monkeys were similar to those in women. The monkey could, thus, serve as a model for the investigation of these new membrane-associated placental tissue proteins.

Animals↗
PubMed1983

Establishment and properties of a human choriocarcinoma cell line of ovarian origin.

A human nongestational choriocarcinoma cell line of ovarian origin (IMa) was established in vitro. This cell line had been subcultured serially more than 22 times over 18 months. Small polygonal cells with a prominent nucleus were dominant and a sparsity of cytoplasmic organelles was an ultrastructural characteristic of the IMa cells. The production and secretion of human chorionic gonadotropin and its subunits were identified by radioimmunoassay. The IMa cells were transplantable in the hamster cheek pouch and the histological diagnosis was choriocarcinoma. A newly established ovarian choriocarcinoma cell line can be considered useful for clarifying the biological differences between nongestational and gestational choriocarcinoma cells.

Animals↗
PubMed1983

Characterization of human choriocarcinoma cell lines secreting high and low amounts of chorionic gonadotropin.

The secretory potential of human chorionic gonadotropin (hCG) in eight strains of human choriocarcinoma cell lines was examined in vitro and the correlation between the secretory potential and certain cell biological characteristics was studied. Multinucleated giant cells (syncytiotrophoblast-like cells) were occasionally observed only in high hCG-secreting cell lines in accordance with inhibition of growth. Appearance of abundant dilated rough endoplasmic reticulum and aggregated glycogen particles in the cytoplasm seemed to represent ultrastructural characteristics of high hCG-secreting cell lines. A shift of nuclear DNA to hyperploidy, its wide distribution, and S phase accumulation of cells by flow cytometry were also characteristic of them. However, no correlation was noted between the hCG secretory potential and population doubling time of the cell lines in vitro.

Cell Line↗
PubMed1983

[Characterization of two choliocarcinoma cell lines and their sensitivity to MTX].

Both the sensitivity to MTX and hCG secretion were compared in two choriocarcinoma cell lines (BeWo and HCCM-5) and study on mechanisms about MTX-resistance was described. The uptake of nucleotides and protein precursors was decreased in dose-dependent manner in both cell lines when exposed to MTX, but HCCM-5 cells showed more sensitivity to MTX. In HCCM-5 cells, both hCG and hCG-beta levels in the medium were decreased with exposure to 10(-9)M MTX, but they tended to increase when exposed to higher concentration of MTX in contrast to the inhibition of 3H-thymidine uptake. However, in BeWo cells, an inverse relationship between the incorporation of 3H-thymidine and secretion of hCG was not clearly observed. The content of dihydrofolate reductase (DHFR) in HCCM-5 cells was about one-half of that in BeWo cells. Although the peak of 3H-MTX incorporation was observed at 120 minutes in both cell lines, HCCM-5 cells incorporated about 4 times higher 3H-MTX than BeWo cells. The difference of sensitivity to MTX between those two cell lines seems to be concerned with both the decrease of MTX transport and increase of intracellular DHFR levels.

Animals↗
PubMed1982

[Studies on marker substances in cell lines derived from various human gynecologic tumors (author's transl)].

In the five kinds of human cultured cells derived from ovarian adenocarcinoma (HOC-21), ovarian malignant teratoma (HOTC 3), carcinoma of the uterine endometrium (HEC-1B), squamous cell carcinoma of the uterine cervix (SKG-1) and choriocarcinoma (BeWo), the intracellular presence of lactic dehydrogenase (LDH), alkaline phosphatase (AlP), human chorionic gonadotropin (HCG), alpha-fetoprotein (AFP) and plasminogen activator were investigated. The results were as follows. 1) BeWo-, HOC-21-and HOTC 3-cells revealed high activity of intracellular presence of lactic dehydrogenase (LDH), alkaline phosphatase (AlP), human chorionic gonadotropin (HCG), alpha-fetoprotein (AFP) and plasminogen activator were investigated. The results were as follows. 1) BeWo-, HOC-21-and HOTC 3-cells revealed high activity of intracellular LDH in this order, however none of HEC-1B-and SKG-1-cells did. 2) The activity of intracellular AlP was higher in BeWo-cells than in HOC-21-cells. The isozymes of AlP detected in these cells were found to be heat-stable. The others revealed no activity of AlP. 3) The presence of HCG-beta was confirmed in both BeWo- and HOTC 3-cells. The intracellular levels of HCG-beta were found to be higher in BeWo- cells than in HOTC 3-cells. HCG-beta was observed to leak into culture medium not from HOTC 3-cells but from BeWo-cells. It was not detected in the other cultured cells. 4) No AFP was detected in any of these five cultured cells. 5) Plasminogen activator was detected in HOC-21, HEC-1B-and SKG-1-cells in contrast to HOTC 3-and BeWo-cells which were negative for plasminogen activator. These results suggest that the various marker substances detected in the human cultured cells originated from various carcinomas of sexual organs may reflect biological functions of these tumor cells and, furthermore, can apply as tumor markers to the clinical diagnosis of the diseases.

Adenocarcinoma↗
PubMed1982

Immunohistochemical detection of pregnancy-specific protein (SP1) and placenta-specific tissue proteins (PP5, PP10, PP11 and PP12) in ovarian adenocarcinomas.

By using an immunoglobulin-enzyme bridge method, pregnancy-specific beta 1-glycoprotein (SP1) and placenta-specific tissue proteins (PP5, PP10, PP11, PP12) were investigated in 15 cases of mucinous cystadenocarcinoma and 20 cases of serous cystadenocarcinoma. These proteins could be detected in the cytoplasm of some malignant cells. In mucinous cystadenocarcinomas, SP1, was present in 53.3% of cases, PP5 in 80.0%, PP10 in 20.0%, PP11 in 66.7% and PP12 in 46.7%. In serous cystadenocarcinomas, SP1 was present in 35.0% of cases, PP5 in 76.2%, PP10 in 9.5%, PP11 in 57.1% and PP12 in 23.8%. No proteins were detected in five normal ovaries. All control sections incubated with phosphate-buffered saline or normal, non-pregnant, non-immune rabbit serum in place of the first specific antiserum as well as with the antisera exhaustively absorbed with the corresponding purified antigens were negative for each protein. The detection rates are discussed in relation to the clinical stages and survival rates of the patients. This study suggests that these proteins, especially PP5 and PP11, may be useful as markers in monitoring patients with ovarian adenocarcinomas as well as for their early diagnosis.

Cystadenocarcinoma↗
PubMed1981

Ectopic production of placenta-"specific" tissue proteins (PP5 and PP11) by malignant breast tumors.

With use of an enzyme-bridge immunoperoxidase (PAP) technique, an immunohistochemical localization of the two new placenta-"specific" tissue proteins, PP5 and PP11, was investigated in 16 cases of breast cancer. PP5 was localized in the cytoplasm of breast cancer cells in 62.5% of cases examined and PP11, in 46.7%. Thus PP5 and PP11 may be markers for breast cancer and the detection of these proteins in sera from breast cancer patients may be more reliable and useful in the detection and monitoring of breast cancer patients than the determination of SP1, PP10, or PP12, other pregnancy "specific" proteins.

Breast Neoplasms↗
PubMed1981

Immunohistochemical location of proteins related to pregnancy-specific beta 1-glycoprotein (SP1) and placental tissue proteins (PP5, PP8, PP9, PP10, PP11, PP12) in the afterbirth of Cynomolgus monkeys (Macaca fascicularis).

The occurrence and location of proteins antigenically related to human pregnancy specific beta 1-glycoprotein (SP1) and to human placental tissue proteins (PP5, PP8, PP9, PP10, PP11, PP12) were studied in the afterbirth (placenta, membranes, decidua, and umbilical cord) of cynomolgus monkeys with use of an immunoglobulin enzyme bridge (PAP) technique. The results were basically the same throughout pregnancy. The syncytiotrophoblast of villi showed clear positive stainings for SP1, PP8, PP9, and PP11, whereas the cytotrophoblast (villi) was found to show clear positive staining for PP9 only. The main sources of proteins related to SP1, PP9, and PP11 were the syncytiotrophoblast cells of the villi and PP8, the syncytiotrophoblast cells of villi as well as the chorionic trophoblast cells. PP12 was mainly located in histiocytes found in the decidua, umbilical cord, amnion, and chorion. There were only traces of PP5 and PP10 in the placentae of cynomolgus monkeys throughout gestation. As in human placentae some granulocyte-like blood cells in the intervillous space were strongly positive for PP8, PP10, and PP12. The findings in the afterbirth of cynomolgus monkeys were similar to those in the human; the monkey could thus serve as a model for the investigation of the new placental proteins.

Animals↗
PubMed1981

Possible synthesis of pregnancy-'specific' beta-1-glycoprotein (sp1) and placenta-'specific' tissue proteins (PP10,PP12) by human and cynomolgus monkey leukocytes.

Using an enzyme-bridge immunoperoxidase (PAP) technique, it was revealed that pregnancy- and/or placenta-specific proteins, SP1, PP10 and PP12 were localized in human and cynomolgus monkey (Macaca fascicularis) polymorphonuclear neutrophils, and PP10, in mononuclear phagocytes. There was no difference in staining results, irrespective of whether the specimens were from human or monkey males, or pregnant or nonpregnant females. The results suggest that SP1, PP10 and PP12 may be synthesized by polymorphonuclear neutrophils and/or mononuclear phagocytes and could represent markers not only for trophoblasts and some kinds of malignant neoplasms but also for normal neutrophils and/or monocytes.

Animals↗
PubMed1981

New placental proteins and their potential diagnostic significance as tumour markers.

Five new soluble placental tissue proteins (PP8, PP9,, PP10, PP11 and PP12) have been isolated, purified and characterized. PP, has the electrophoretic mobility of a beta 1-globulin; the other 4 proteins are alpha 1-globulins. The molecular weights of these proteins range from 25 000--65 000 daltons. They all are glycoproteins with carbohydrate contents ranging from 3.9--6.6%. The amino acid compositions of these proteins also have been determined. PP8 and PP, are ubiquitous tissue proteins in that they occur in relatively high concentrations in almost all human tissues examined. PP10, PP11 and PP12 could not be detected in extracts of other human tissues and, therefore, were supposed to be specific to the placenta. PP10--12 was shown to be present in tumour tissues by an immunoglobulin enzyme bridge (PAP) technique. The results suggest that these proteins may be useful as markers in oncology.

Animals↗