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Biomedical subjects

N Ibaraki

Publications and source records attributed to N Ibaraki.

30 records · Page 2Linked to original sources
PubMed1993

Explant culture of human lens epithelial cells from senile cataract patients.

Human lens epithelial cells (HLECs) with lens capsules were cultured to investigate the morphology and outgrowth potential of the cultured cells. Anterior lens capsules obtained during cataract surgery were placed cell side down in a medium in culture wells. Cultivation was successful with 24 of 36 specimens from senile cataract patients. The HLECs grew beyond the capsular edge from the third to fifth day after the beginning of cultivation, and extended actively to the periphery of the culture well. The cells had extended 2.5 mm beyond the capsular edge by the second week and 3.8 mm by the fourth week, but outgrowth then stopped. The cells near the capsular edge displayed elongated shapes during the earlier period of cultivation, but subsequently formed small polygons. The cells in the middle of the zone of outgrowth appeared as polygons, and were surrounded by large, irregularly-shaped cells. This study showed that HLECs from senile cataract patients have a limited but definitive outgrowth potential under explant culture conditions.

Aged↗
PubMed1993

[Histopathological study of rabbit anterior capsule opacification after IOL surgery].

We investigated the histopathology of anterior capsular opacification after intraocular lens (IOL) implantation in a rabbit eye. Sixteen eyes of eight albino rabbits underwent continuous circular capsulorhexis, phacoemulsification, and in-the-bag IOL implantation. The anterior capsules were examined by slitlamp, light microscopy, and transmission electron microscopy. Anterior capsular opacification during the fourth week after surgery appeared similar to that of the human eye. Multi-layered lens epithelial cells and collagenous fibers were recognized in the anterior capsular opacification. New lens fibers, however, extended between the anterior capsules and the IOLs, and caused the opacification to become obscure. This model may be useful for investigating anterior capsular opacification after cataract surgery during the early postoperative period.

Animals↗
PubMed1992

Anterior capsule opacification: a cell culture model.

We cultured the human lens epithelial cells with attaching anterior lens capsule using surgically removed capsular flaps obtained in intraocular lens implantation. The capsular flap was placed with cell side down in a small culture well. In phase contrast microscopy, an anti-meniscus plate was placed on the bathing medium to avoid optical aberration. The human lens epithelial cells showed outgrowth which ceased after 3 to 4 weeks of incubation. The cells under the capsule lost distinctive cell margin. The cells outgrew from the capsular edge both onto the anterior capsular surface and the well bottom. The outgrowth length and Bromodeoxyuridine uptake indicated a low proliferative potency of the human lens epithelial cells.

Cataract↗
PubMed1992

[Ciliary body changes associate with aldose reductase in galactosemic rats (2)].

Morphological changes of the ciliary body epithelium in rats fed a 50% galactose diet for 18 months with or without aldose reductase inhibitor (ARI: FR74366, Fujisawa) were investigated on coronal sections by both the light microscopic observation and the measurement of the area of non-pigmented epithelium (non-PE) as the ratio of non-PE/PE. The measurement was performed with color image analyzer. Structural change (hypertrophy) was observed only in non-pigmented epithelial cells of galactosemic rats. In contrast, no hypertrophic changes were seen in galactosemic rats treated with FR74366. These results strongly suggest that aldose reductase is associated with hypertrophic change of non-pigmented epithelial cells of the ciliary body as one of the diabetic complications.

Aldehyde Reductase↗
PubMed1991

[Changes in cell kinetics of rat lens epithelial cells with aging].

Whole-mount preparations of developing rat lens epithelium were made and 3H-thymidine autoradiography was performed on them. The distribution of 3H-thymidine-labeled (DNA-synthesizing) epithelial cells was examined by the above combined methods. Labeled cells were evenly distributed in the epithelial cell layer (not the definitely germinal zone) until 1 week after birth. In 3- or 7-week-old rat lens, labeled cells were mainly distributed in the germinal zone, and the number of labeled cells localized in the anterior subcapsular epithelial cell layer decreased according to age of the rat. The 24-week-old rat showed localization of labeled cells at the germinal zone, which seemed to be similar to the human adult lens.

Aging↗
PubMedDate not supplied

Age-related changes of human lens epithelial cells in vivo.

We examined the density and morphology of lens epithelial cells (LECs) in vivo in a group of normal volunteers and cataract patients by using a newly developed noncontact specular microscope. There was a statistically significant decrease in the cell density of LECs in a group of cataract patients over the age of 80 years. The coefficient of variation of the cell area and the number of large black spots that were observed in the enhanced specular images were not related to aging or cataract formation. Our data indicate that the cell density of LECs decreases after reaching the age of 80, but cataract formation does not affect the cell density or the coefficient of variation of the cell area until the age of 80.

Adolescent↗