Search PubMed⌕ Search

Biomedical subjects

N ISHIDA

Publications and source records attributed to N ISHIDA.

At least 55 records · Page 3Linked to original sources
PubMed1956

Growth characteristics of influenza virus; properties of the initial cell-virus complex.

The rate has been determined at which the initial stable complex is formed between cells of the chorioallantoic membrane and the PR8 strain of type A influenza virus. Characterization of the complex revealed the following properties: (a) stability to dilution and to simple washing with buffered saline, (b) insensitivity to the action of receptor-destroying enzyme (obtained from cholera vibrio), (c) sensitivity to the inhibitory effect of anti-viral immune serum, (d) formation of the complex at low temperatures, 3 degrees C., (e) accompanied by a loss of detectable viral infectivity from the reaction mixture, (f) stability at 3 degrees C. but transformation at 37 degrees C. to a state which is resistant to the inhibitory action of a specific anti-viral serum. The rate of this latter transformation has been determined. The infectious process in more than 50 per cent of the cells can be influenced by immune serum 2 hours after the completion of the initial reaction with virus.

Influenza A virus↗
PubMed1955

Growth characteristics of influenza virus concerning the binding of virus by host cells.

Under certain conditions, influenza virus may bind to chorioallantoic membrane and the infectious property is retained upon prolonged incubation of the complex. Apparently the bound active virus is not functioning in the initiation of viral increase. The bound infectious virus may be partially removed by extensive washing. The characteristics of the washing are suggestive of a reversible equilibrium type of binding. Binding will also occur when the tissue has been pretreated with RDE or in the presence of AMPS. However, under these conditions the binding is of a lesser degree. When the tissue has been treated with RDE and AMPS is present, no stable binding occurs. In the presence of AMPS, the initiating activity is bound but cannot function in promoting viral increase. It is proposed that active virus is held by two types of binding at the same site; one type of binding is sensitive to the action of RDE; the second type is sensitive to the blocking effect of AMPS. Virus can be held to the receptor site by either type of binding or both. It is further suggested that the bound infectious virus is a result of an abortive attempt at initiating infection. The nature of the binding of infectious virus is of significance for understanding the binding of initiating activity.

Binding Sites↗