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Biomedical subjects

N I Kondrikov

Publications and source records attributed to N I Kondrikov.

At least 19 recordsLinked to original sources
PubMed2000

[Quantitative evaluation of the activity of nuclear Ca2+/Mg2+-dependent endonucleases in hyperplasia and cancer of the endometrium].

Endometrial carcinoma is the most incident cancer of human reproductive system. There are unequivocal evidences of relationship between complex and atypical hyperplasia and development of cancer. Apoptosis plays a significant role in the maintenance of equilibrium between cell death and proliferation and contributes to prevention of tumorigenesis. Internucleosomal DNA fragmentation known as one of the most important criteria of apoptosis cannot be used for evaluating the risk of cancer development because it reflects the current level of apoptosis but is useless for evaluating the real limits of apoptosis intensity in certain types of tissue. For estimating the possibility of apoptosis development in endometrial tissues, a new method of quantitation of nuclear Ca(2+)/Mg(2+)-dependent endonuclease (NCME) activity has been developed. Fifteen samples of normal endometrial tissues at middle proliferative, secretory, premenstrual, and menstrual phases, 43 samples of hyperplastic endometrial tissues, 13 samples of endometrial polyps, and 17 samples of endometrial adenocarcinoma were collected by diagnostic curettage of the uterine cavity and by hysterectomy (carcinomas). The material was examined by 1) TUNEL method and 2) agarose gel electrophoresis of DNA cleaved by nuclear CME in isolated cell nuclei in the presence of Ca(2+) and Mg(2+) ions, followed by quantitation of CME activity. The activity of NCME was found to decrease from normal endometrium (1.1 +/- 0.12 U, without significant changes throughout the menstrual cycle) through polyps (0.9 +/- 0.15 U), cystic hyperplasia (0.45 +/- 0.06 U, p < 0.01), and adenomatous hyperplasia (0.32 +/- 0.08 U, p < 0.01) to adenocarcinoma (0.37 +/- 0.11 U, p < 0.01 for well differentiated, 0.16 +/- 0.08 U, p < 0.01 for moderately differentiated, and 0.03 +/- 0.02 U, p < 0.01 for poorly differentiated samples). The TUNEL-specific staining pattern in normal endometrium varied in a wide range during the menstrual cycle (from poorly stained individual cells in the proliferative phase to intensely stained cell clusters in the premenstrual phase). At the same time the difference between the normal endometrium in the proliferative phase and pathologically changed endometrium (hyperplasia or cancer) could not be detected by the TUNEL technique. Hence, TUNEL is useless for predicting cancer development in hyperplasia and precancer. By contrast, evaluation of NCME activity helps detect the early disorders in the proliferative processes coursing in endometrial tissues and thus prevent tumor development.

Adenocarcinoma↗
PubMed1995

[The clinical importance of the dopplerometric study of the blood flow in the normal iliac, uterine and ovarian arteries, in myoma and in endometriosis interna of the corpus uteri].

Dopplerometric investigation of pelvic arteries was carried out in 30 women without gynecologic diseases, in 51 patients with internal endometriosis, and 48 ones with uterine myomas. Internal endometriosis of the uterus was found to be characterized by specific qualitative changes in spectral curves of bloodstream velocity in the internal iliac and uterine arteries. Moreover, progressive changes in spectral curves related to dissemination of endometrioid heterotopias were revealed. In patients with uterine myomas combined with internal endometriosis disorders of peripheral circulation typical of endometriosis predominated.

Adult↗
PubMed1994

[Current concepts in observation and treatment of patients with internal endometriosis of the uterus].

The paper outlines the issues of improving and systematizing clinical instrumental diagnostic and therapeutical techniques for internal endometriosis. The results of examining 220 patients with internal endometriosis, out of whom 116 were further operated on, have been prospectively and retrospectively studies. Based on the histological findings of the gross specimens intraoperatively removed, the authors analyzed the predictive value of clinical and instrumental diagnostic criteria, including those of transvaginal echography, Doppler echometry of the arteries supplying the uterus, those of hysteroscopy and the computer hysterosalping graphic technique developed by them. The ovarian morphological and receptor apparatuses were studied in internal endometriosis. The examination and treatment system for patients with internal endometriosis is presented in the paper.

Adolescent↗
PubMed1994

[Morphofunctional characteristics of the placenta of women living in extreme conditions of the Aral cost line].

Morphologic examination of the placenta from 41 puerperants living near the Aral sea revealed an increase of its mass and changes in other parameters. The detected pathologic processes: hemodynamic disorders and dystrophic changes--are not specific and contribute to the development of placental insufficiency. The majority (85.6%) of newborns were born in asphyxia. Morphofunctional features of the placentas of multiparous women and the status of their children permit regarding this population as the best adapted to pregnancy and delivery of viable children under the said extreme conditions.

Adaptation, Physiological↗
PubMed1994

[Experimental validation of the efficacy of laser-magnetic therapy for chronic placental insufficiency].

A new pathogenetically based non-medicamentous method for correction of uteroplacental bloodflow disturbances has been developed on the model of chronic placental insufficiency in rats. A single 5 min laser-magnetic exposure on day 21 of normal pregnancy resulted in a vasodilating effect with reduction of the peripheral resistance in the uterine horn vessels and with improvement of their blood supply. A new LAMA laser magneto-therapeutic device was employed. Daily 5 min sessions of laser magnetic therapy administered to rats with chronic placental insufficiency from pregnancy days 15-16 to 21 normalized uterine horn contractility and resulted in positive morphofunctional changes in the components of the uterine horns and placenta, being associated with a noticeable improvement of fetal functions. Hence, laser magnetic therapy may be regarded as an effective non-drug method for therapy of chronic placental insufficiency.

Animals↗
PubMed1994

[Endometrial and ovarian morphology in internal uterine endometriosis].

The ovaries and endometrium were examined in 66 patients with internal uterine endometriosis with various degrees of the process dissemination. A high incidence of combinations of uterine endometriosis with hyperplastic processes in the ovaries was revealed (72.7%) and a relatively low incidence of uterine body mucosa involvement (31.7%). The degree of endometriosis dissemination in the myometrium and the incidence of ovarian hyperplasia were in direct proportion. The authors emphasize the usefulness of morphologic studies of the ovaries in patients with internal uterine endometriosis.

Adult↗
PubMed1993

[The clinico-morphological and cytological characteristics of the ecto- and endocervix with the use of the oral contraceptive triregol].

Cervix uteri status was examined in 55 young nulliparous women in the course of triphasic oral contraception. The status of the cervix uteri was assessed on the basis of clinical, colposcopic, cytologic, and morphofunctional examinations. Triphase oral contraception with triregol was found conducive to the formation of squamous-cell metaplasia in ectopic sites, this promoting epithelialization of the cervix uteri.

Adolescent↗
PubMed1993

[The ultrastructure and electron-histochemical characteristics of the stromal cells of the endometrium].

Histologic, histochemical, electron-microscopic and ultracytochemical studies of cellular populations of the endometrial stroma and the vascular component, carried out in 33 women of a productive age in various phases of the cycle (on days 5-28) showed interactions of the cellular elements (fibroblast-like cells, predecidual cells, endometrial granulocytes, endotheliocytes, cells of a hematogenic origin) within and between the populations over the course of the cycle; all the cells were found functionally heterogenic, this underlying the endometrial homeostasis maintenance. Disturbances in any of the components of these interactions may result in a pathologic process in the uterine body mucosa.

Adult↗
PubMed1990

[Alternative solutions in the treatment of patients with combined benign endometrial and myometrial pathology].

The study included 96 women with combined benign hyperplastic uterine conditions. Half of them underwent a radical uterine resection and were examined at 3.1 +/- 0.2 years following the operation. The rest were randomly selected as matched pairs and were evaluated at 4.3 +/- 0.13 years following multicomponent treatment including hormones (n-26) and cryohormonal treatment (n-22). Analysis of results revealed a complex pathogenesis of this disease of the reproductive tract, presenting as severe postoperative vegetoneurotic disorders. Potential of an adequate conservative treatment in preserving specific female functions was determined.

Adult↗
PubMed1990

[Effectiveness of hormonal therapy of endometrioid ovarian cysts].

Patients with ovarian endometriosis have been examined during the follicular and luteal phase of the ovulatory and the second phase of the anovulatory cycles. Estradiol and progesterone blood concentrations measured on operation days and during the respective ovulatory phases were in a normal range. Ovulation and endometrial hyperplasia reduced basal progesterone concentrations. Comparison of findings on steroid receptor systems in the endometrium and endometrioid ovarian cysts indicated that they were identical. The findings were different in ovulation and endometrial hyperplasia. These patterns provided criteria for endometrial response to female sex hormones by assaying endometrial intracellular steroid receptor systems in normally ovulating patients with ovarian endometriosis.

Adult↗
PubMed1989

[Clinico-morphological characteristics of retrocervical endometriosis].

Clinico-morphologic parameters are described in 18 patients of reproductive age before and after hormonal treatment. There was no clear-cut correlation between morphofunctional endometrial state and endometrioid heterotopies. It did not seem possible to identify the phase of the cycle on the basis of the structural pattern of retrocervical endometriosis. Electron microscopic findings in endometriosis sites were suggestive of considerable variability of epithelial cell activity in different glands as well as within the same gland. Post-treatment structural pattern of retrocervical endometriosis does not exclude the possibility that steroids only block cyclic changes in the functional glandular epithelium, having no effect on nonactive cell elements. In spite of dystrophic changes, present in many retrocervical endometriosis sites, hormonal treatment did not eliminate endometrioid heterotopies, but only relieved pain.

Adult↗