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Biomedical subjects

N Horie

Publications and source records attributed to N Horie.

At least 55 records · Page 3Linked to original sources
PubMed1999

Intraosseous squamous cell carcinoma arising in association with a squamous odontogenic tumour of the mandible.

We report a rare occurrence of intraosseous squamous cell carcinoma (SCC) arising in association with a squamous odontogenic tumour (SOT), which had not previously been documented in the literature. A 53-year-old man had, for 5 years, a well-demarcated radiolucency attached to the impacted third molar of the mandible. The enucleated specimen had a characteristic pattern of SOT, but in which a few epithelial islands showed atypical features suggestive of SCC. Intense p53-, proliferating cell nuclear antigen- and Ki-67-positive cells were detected in carcinoma areas. Within 2 months, aggressive bone destruction showing typical findings of intraosseous SCC appeared. The present tumour is presumably a malignant variant of SOT.

Ameloblastoma↗
PubMed1999

Collagenous fibroma (desmoplastic fibroblastoma) presenting as a parotid mass.

We describe the case of a 50-year-old man who was found to have a painless, slow-growing parotid mass of 10 months duration. At surgery, a well-delimited, lobulated 4x5-cm tumor was located on the deep lobe of the left parotid gland, adhering to the parotid sheath and masseteric fascia. Follow-up 6 years later has shown no evidence of recurrence. Cardinal morphologic features included multiple nodules of sparsely distributed stellate-or spindle-shaped fibroblasts within a collagenous or myxocollagenous stroma. Fascial involvement and entrapment of salivary gland were focally identified at the edges. Tumor cells were diffusely positive for vimentin with faint focal staining for alpha-smooth muscle actin. These findings satisfied the diagnostic criteria for collagenous fibroma (desmoplastic fibroblastoma). Ours represents the first report of this tumor type mimicking a parotid tumor.

Fibroma, Desmoplastic↗
PubMed1999

Primary intraosseous carcinoma of the mandible with probable origin from reduced enamel epithelium.

This report describes a case of primary intraosseous carcinoma (PIOC) arising de novo in the mandible. The patient was a 74-year-old woman and an early PIOC was found incidentally during histopathological examination of the pericoronal tissue obtained at extraction of a deeply impacted third molar. The curetted soft tissues consisted of a microinvasive, keratinizing squamous cell carcinoma with scattered foci of carcinoma cells showing calcification; stromal osseous metaplasia was also observed. After additional treatment, the patient has remained free of disease for 2 years. Since the tumor was completely enclosed in the bone, the only identifiable source of the present PIOC is the reduced enamel epithelium. Despite its rarity, this case emphasizes the importance of careful histologic examination of all apparently innocuous dental follicles that are removed surgically.

Aged↗
PubMed1999

A case of an electrical burn in the oral cavity of an adult.

Electrical burns in the oral cavity account for 2.2% of all electrical burns and only 0.12% of all burns; thus, the incidence of electrical burns in the oral cavity is relatively low. As this type of injury occurs in the oral cavity when an individual sucks or chews on a live electrical wire, extension cord, plug, or outlet, most cases occur in toddlers or preschool children, and adult cases are extremely rare. Here we describe a case of an electrical burn in a 56-year-old man who accidentally bit the electric wire of a cleaner while carrying out repairs. Conservative treatment, without surgery, was performed. Two years after the injury, a slight scar and a small tongue deformity remain, but no functional disturbance has been observed.

Burns, Electric↗
PubMed1999

So-called simple bone cyst of the jaw: a family of pseudocysts of diverse nature and etiology.

The nature and etiology of so-called simple bone cyst (SBC) are still a subject of debate. Our comprehensive review of the literature suggests that SBC, which appears histologically to be a single entity, has different natures and etiologies, resulting in divergent clinical features. In addition, an interesting case of mandibular SBC in an 11-year-old girl is presented with details of radiographic changes over a 7-year period. Fully documented patient records revealed that this lesion originated in the apical area of the first molar and took about 4 years to develop into a clinically evident bony expansion.

Child, Preschool↗
PubMed1998

Angiomyolipomatous hamartoma of the tongue.

Extrarenal angiomyolipoma is a rare subtype of benign lipomatous tumor composed of adipose tissue, blood vessels, and smooth muscle. We report a 60-year-old woman who developed an angiomyolipomatous tumor of the tongue and discuss the differential diagnosis along with a review of the literature. The term angiomyolipomatous hamartoma is proposed for the present lesion to distinguish it from classic oral angiomyolipoma.

Actins↗
PubMed1998

Intravenous myofibroblastic pseudotumour of the buccal mucosa.

An intravenous pseudotumour at a traumatised site of the buccal mucosa of a 68-year-old woman is described. The lesion was composed of reactive spindle cell proliferation, merging of haphazard bizarre cells and inflammatory elements. Cellular morphology and immunohistochemical profile of proliferating cells reflected that of myofibroblasts. Conceivably, these cells may be derived from indigenous vascular smooth muscle cells. The present case represents a unique type of a reparative process intimately related to the tissue damage.

Aged↗
PubMed1998

Inflammatory pseudotumor in the mandibular retromolar region.

An unusual pseudosarcomatous lesion of the oral mucosa is described in a 43-year-old woman. It presented as an aggressive ulcerative mass in the mandibular retromolar gingiva of one month's duration. The lesion was composed of florid myofibroblastic and fibrohistiocytic proliferations with infiltrative margins in an inflammatory background and was considered to be an inflammatory pseudotumor. Although the tumor appeared to have been incompletely excised, the patient remains free of disease at 1 year follow-up.

Adult↗
PubMed1998

Cloning and characterization of the promoter region of monkey thymidylate synthase gene.

Thymidylate synthase (TS) catalyzes the conversion of deoxyuridylate to thymidylate. Promoter regions of the TS gene from three vertebrates, human, mouse and rat, have been analyzed so far. A unique inverted repeat was present in the promoter region of the human, but not in the mouse and rat, TS genes, and the feature of the promoter region is considerably different between the human TS gene and the two rodent TS genes. To examine whether the characteristics of the human TS gene promoter is intrinsic to human or primate, we cloned a DNA fragment containing the promoter region of the monkey TS gene and determined the nucleotide sequence in this region. The nucleotide sequence in the promoter region of the monkey TS gene was revealed to have 88% homology with that in the corresponding region of the human TS gene. The unique repeated structure mentioned above was also found in the monkey TS gene. Furthermore, the Sp1-binding motif and the CACCC box were found to be conserved in the monkey TS gene. The result of chloramphenicol acetyltransferase assay suggested that the Sp1-binding motif and the CACCC box are essential for the promoter activity of the monkey TS gene, as in the case of the human TS gene.

Animals↗
PubMed1998

Purification method for human plasma kallikrein by a new affinity chromatography.

We synthesized a new affinity gel (PKSI-Toyopearl) using a selective synthetic inhibitor of plasma kallikrein (PKSI-527) as an affinity ligand, and employed it for the rapid purification of plasma kallikrein from human plasma. Human plasma activated with kaolin after acid treatment was applied to a PKSI-Toyopearl column. Adsorbed protein was eluted with 50 mM glycine-hydrochloric acid buffer (pH 3.0). Plasma kallikrein was purified 181-fold with a yield of 85% from the kaolin-activated plasma. Further purification was performed by chromatography on a DEAE-Toyopearl 650M column. Plasma kallikrein was finally purified 1720-fold with a 63% yield by these procedures. On sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis, a band was observed at approximately 88 kDa. These findings indicate that PKSI-Toyopearl is a valuable tool for the purification of plasma kallikrein from human plasma.

Blood↗
PubMed1998

Rapid diagnosis of oral tuberculosis by amplification of Mycobacterium DNA from paraffin embedded specimens.

The polymerase chain reaction (PCR) assay is a powerful tool for quick diagnosis of various infectious diseases. We applied this technique as well as conventional histopathological examination to diagnose oral tuberculosis. Ziehl-Neelsen staining of oral mucosal specimens often fails to detect Mycobacterium (M.) tuberculosis due to the low number of bacteria in the tissue. Specific primers and probes were synthesized based upon the nucleotide sequence of the 65 kDa membrane protein of M. tuberculosis. DNA extracted from the paraffin-embedded tissue was amplified using taq polymerase. PCR assay detected M. tuberculosis in 5 of 6 samples. Although the gene segments from these species were quite similar, the gamma 32P labeled noligonucleotide probes distinguished between M. tuberculosis and M. fotuitum by southern blot hybridization. In all specimens that were Ziehl-Neelsen negative, M. tuberculosis DNA was detected by PCR. These results suggest that PCR is a useful means of diagnosing mycobacterium infection.

Adult↗
PubMed1997

Identification of functional elements in the promoter region of the human gene for thymidylate synthase and nuclear factors that regulate the expression of the gene.

To identify the essential motifs of the promoter of the human gene for thymidylate synthase (TS), we constructed a set of deletion mutants from the 5'-terminal region of the human TS gene. From the results of assays of the expression of chloramphenicol acetyltransferase (CAT), we identified two functional elements with positive effects on the promoter activity: a CACCC box (CCACACCC) and an Sp1-binding motif (GAGGCGGA) that was homologous to the Sp1-binding site in the mouse TS gene. In addition, negative regulatory sequences were identified between the two positive elements and in the region upstream of the CACCC box. The results of gel mobility shift analyses suggested that Sp1 binds to the Sp1-binding motif of the human TS gene promoter and that multiple nuclear factors that are related to Sp1 bind to the CACCC box. Furthermore, the binding of Sp1 to mutated Sp1-binding motifs in the promoter region of the human TS gene was correlated with the promoter activity, as measured by the CAT assay. Therefore, the Sp1 motif seems to be a major contributor to the basic promoter activity of the human TS gene, although multiple positive and negative regulatory elements are involved in the regulated expression of this gene.

Animals↗
PubMed1997

Basaloid squamous cell carcinoma of the esophagus metastatic to the gingiva: a case report.

Basaloid squamous cell carcinoma (BSCC), a biologically high-grade variant of squamous cell carcinoma, carries a grave prognosis. In this article, we describe a rare case of BSCC of the esophagus with metastasis to the mandibular gingiva in a 63-year-old man. In addition, we provide a review of the relevant literature along with a discussion of the differential diagnosis.

Carcinoma, Basosquamous↗
PubMed1997

Glial choristoma in the oral cavity: histopathologic and immunohistochemical features.

This report describes the morphologic and immunohistochemical features of two cases of glial choristoma arising in the palate. The bulk of the lesions consisted of mature neuroglial tissue admixed with salivary glands, adipose tissue and lymphoid aggregates in case 1 and of cartilage, bone and foci of smooth muscle cells in case 2. In addition, case 2 showed choroid plexus and melanin-containing epithelium. Neuroglial tissue was intensely positive for CD57 as well as for glial fibrillary acidic protein, S-100 protein and vimentin. Neuron-specific enolase and neurofilament were focally or weakly positive. Proliferating cell nuclear antigen was negative in case 1 but sparsely positive in case 2. These features reflect the fact that glial choristoma is a developmental malformation of heterotopic central nervous tissue with limited growth potential.

Adipose Tissue↗
PubMed1997

Fumaric acid, anti-thrombin substance from Rhizopus javanicus.

An anti-thrombin substance (M2) was isolated from a culture broth of Rhizopus javanicus. Accumulation of M2 reached a maximum peak after 14 to 15 days of incubation and then decreased. The yield of M2 was 500 mg from 1 L of culture broth. M2 inhibited thrombin activity, and its 50% inhibition concentration in a reaction mixture containing 50 microL of 12.5 NIH unit/mL thrombin and 200 microL of 0.33% bovine fibrinogen was 63 microM. M2 had a specific activity for thrombin, but it was less responsive to plasmin, tissue-type plasminogen activator, urokinase, plasma kallikrein and glandular kallikrein. The structure of M2 was identified as fumaric acid by elementary analysis, FAB/MS, 1H-NMR, 13C-NMR and IR spectra.

Animals↗
PubMed1996

Use of an active center-directed plasmin inhibitor elucidates the multiplicity of plasmin actions.

In our studies, designed to synthesize an active center-directed plasmin (PL) inhibitor, N-(4-aminomethylbenzoyl)-4-(3-picolyloxy)-L-phenylalanine n-hexylamide dihydrochloride (PASI-535) was found. We characterized PASI-535 and analyzed the actions of PL, comparing with those of PASI-535 and tranexamic acid (t-AMCHA). (1) PASI-535 strongly inhibited not only fibrinolysis (IC50: 2.9 x 10(-6) M) but also amidolysis (Ki value: 2.9 x 10(-6) M) and fibrinogenolysis (IC50: 4.5 x 10(-6) M) induced by PL. While t-AMCHA which strongly inhibited fibrinolysis (IC50: 6.0 x 10(-5) M), rarely inhibited amidolysis (Ki value: 4.0 x 10(-2) M) and fibrinogenolysis (IC50: 1.0 x 10(-2) M). (2) PL is able to liberate kinins by degrading kininogen. This kinin-generation by PL was inhibited by 2 x 10(-5) M PASI-535. However, it was little inhibited even by 1 x 10(-3) M t-AMCHA. (3) The inhibitory effect of PASI-535 and t-AMCHA on tumor growth was studied. In sarcoma-180 bearing mice, ascites retention and the increase of tumor cells were markedly suppressed by subcutaneous injection of PASI-535, either 30 mg/kg/day or 50 mg/kg/day, for 5 days, and the inhibitory effect was dose-dependent. Although t-AMCHA also reduced both ascites retention and the increase of tumor cells, it needed approximately 40 times (2 g/kg/day) the amount of PASI-535 to exert these effects. PASI-535 may be a useful tool in analyzing the multiplicity of PL actions. Moreover, PASI-535 can be used as an antifibrinolytic drug which has a mechanism of function different from that of t-AMCHA.

Amino Acid Sequence↗