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Biomedical subjects

N Hisanaga

Publications and source records attributed to N Hisanaga.

At least 37 records · Page 2Linked to original sources
PubMed1998

Effect of working hours on biological functions related to cardiovascular system among salesmen in a machinery manufacturing company.

A field survey of 71 salesmen (22-60 years) in a machinery manufacturing company was conducted to investigate the effect of working hours on biological functions related to the cardiovascular system. The subjects were divided into four groups by age, and those in each age group were further divided into shorter (SWH) and longer (LWH) working hour subgroups by weekly working hours. Rates of complaints of subjective fatigue for LWH were significantly higher than those for SWH on the whole. Although the mean amplitude of respiratory sinus arrhythmia at rest decreased with age, no significant difference between SWH and LWH was found in this function. Systolic blood pressure for LWH was significantly higher than that for SWH in the 50-60 year group. The serum total cholesterol level for LWH was significantly lower than that for SWH in the 40-49 year group. Comparison of biological functions related to cardiovascular system was also made between fatigue complaint and no-complaint subgroups. Significant differences were found between the two subgroups: systolic blood pressure was higher and the total cholesterol level was lower in the fatigue complaint subgroups. Summarizing our results, it appeared that long working hours might increase systolic blood pressure and lower the total cholesterol level due to fatigue.

Adult↗
PubMed1998

[Types and dimensions of mineral fibers in the atmosphere and from lungs of residents in the urban area].

OBJECTIVE: The objective of this study was to estimate the types and origin of the mineral fibers in the lungs of residents in the urban area. METHODS: Mineral fibers in the atmosphere from 21 urban areas were analyzed by direct transfer-analytical transmission electron microscopy (ATEM). Lung tissues of 36 urban residents without occupational asbestos exposure were also analyzed by ATEM after low temperature ashing. Measurement of dimensions of all types of mineral fibers, identification of asbestos, and classification of nonasbestos fibers according to the combination of main elements were carried out. RESULTS: 1. Chrysotile, amosite, actinolite, tremolite and 11 types of nonasbestos fibers were found in both the lung tissues and the atmospheric samples. The fibers, mainly composed of Al and Si, contributed 16.3% and 62.3% of the atmospheric samples and lung tissues respectively. On the other hand, the proportion of the fibers mainly composed of S and Ca were 43.7% and 0.0% in the atmosphere and lung tissues respectively. Those fibers mainly composed of P and Fe were 0.4% and 18.4% in the atmosphere and lung tissues respectively. 2. Fifteen types of nonasbestos fibers were found exclusively in the atmosphere. The average proportions for ten of these were less than 0.5% each. 3. Crocidolite was found exclusively in the lung tissues. 4. No significant difference in the geometric mean of length and diameter of asbestos was found between the atmospheric samples and lung tissues. Some types of nonasbestos fibers had significantly larger length and diameter in the atmospheres than in the lung tissues (p < 0.01). CONCLUSION: The results suggest that mineral fibers found in lung tissues could be classified into three groups on the basis of their behavior. 1) Some fibers, such as amphibole asbestos and fibers composed of Al and Si, are inhaled from the atmosphere and remain in the lung tissues for a long time. 2) Some fibers, such as those composed of S and Ca, are inhaled from the atmosphere and eliminated from the lung tissues within a relatively short time. 3) Some fibers found more in the lung tissues than in the atmospheres, such as those composed of P and Fe, are suspected to be generated in the lungs.

Air Pollutants↗
PubMed1997

A cohort mortality study of construction workers.

This report presents a mortality study among the 17,344 members of the Construction Workers' Health Insurance Society of Mie Prefecture in Japan. The study period was between 1973 and 1993. During this period, 480 members died. Age-specific mortality rates of Mie Prefecture were used as comparison standards. Significantly elevated standardized mortality ratio (SMR) and proportionate mortality ratio (PMR) were observed for "accidents and adverse effects." In addition, the PMRs of all cancers and "cancers of trachea, bronchus and lung" were also significantly elevated. The job classifications were reorganized into three groups, according to the frequency of asbestos exposure the workers experienced on the construction sites. The asbestos exposure was based on job classifications among 7,411 workers who had completed a self-administered survey questionnaire. In the frequent-exposure group, the PMR was significantly elevated for all cancers. In the medium-exposure group, the SMRs were significantly elevated for all cancers and "cancer of trachea, bronchus and lung." The PMR was significantly elevated for "cancer of trachea, bronchus and lung." In the less-exposure, group, the PMR was significantly elevated for "accidents and adverse effects." This study provided support for the hypothesis that working in the construction industry might be associated with high risks for asbestos-associated cancers and accidental deaths.

Accidents, Occupational↗
PubMed1997

Pulmonary metal distribution in urban dwellers.

To clarify the factors influencing the concentrations and distribution of metal elements in the lung, we analyzed the following 8 metals in the 5 lung lobes of 17 autopsied urban dwellers by flame or flameless atomic absorption spectrometry: aluminum (Al), cadmium (Cd), chromium (Cr), nickel (Ni), lead (Pb), manganese (Mn), copper (Cu), and Zinc (Zn). The arithmetic mean value (in micrograms of metal per gram of dry weight) calculated for five lobar metal concentrations in each subject ranged from 80 to 681 for Al, from 0.34 to 3.41 for Cd, from 0.41 to 12.7 for Cr, from 0.22 to 1.93 for Ni, from 0.15 to 1.47 for Pb, from 0.64 to 2.36 for Mn, from 2.84 to 7.24 for Cu, and from 40.7 to 77.6 for Zn. The eight metals were classifiable into two groups on the basis of the interindividual and interlobar concentration variations. The first group, consisting of Cu and Zn, was characterized by smaller interindividual variations (co-efficient of variation, CV < 30%) and smaller inter lobar variations (mean CV < 15%). Their levels were not affected significantly by sex, smoking habits, or possible occupational exposure to metal-containing dust. In contrast, the second group, consisting of Al, Cd, Cr, Ni, Pb, and Mn, exhibited larger interindividual variations (CV > 45%) and larger interlobar variations (mean CV > 25%). Concentrations of these metals, except for Cd, tended to be higher in the upper lobes than in the lower lobes. The concentrations of Al and Cr were significantly higher in men than in women. Smoking significantly elevated the levels of Cr, Cd, Ni, and Pb, and the concentrations of Al, Cr, Ni, and Mn were higher in subjects with possible occupational exposure to metal-containing dust than in those without such exposure. However, the interlobar distribution patterns of the metals did not seem to be influenced by sex, smoking habits, or occupational exposure to metal-containing dust.

Adult↗
PubMed1997

The effect of a back belt on torso motion--survey in an express package delivery company.

According to the Labor Standard Bureau of Japan, accidental back injuries accounted for about 60% of all occupational injuries and diseases in the last decade. The Ministry of Labor issued guidelines to prevent low back injuries in 1994 to address the problem. The use of back belts is recommended for some special working conditions but details on its proper use were not given. This study was planned to evaluate a newly developed back belt and was done at an express package delivery company where the incidence of low back injury was high. The BackTracker was used to evaluate the effect of the back belt on the range and velocity of torso motion. The results indicated that there were no significant differences in the range of motion (ROM) during flexion/extension, lateral bending, and rotation between with and without the belt. The maximum angular velocity (MAV) of flexion decreased significantly (average decrease: 30 +/- 28.3 degrees/sec) when the back belt was worn. The MAV of extension with belt showed a decreasing tendency though not significant. No notable trends were observed in the MAV, during lateral bending and rotation of the subjects while wearing and not wearing the belt. The results also indicated that the back belt affected differently the torso motion of each subject. This study suggested that this back belt could be useful for tasks with high velocity of flexion/extension and that proper instruction on the use of the back belt is needed for each worker.

Adult↗
PubMed1997

Sitting posture and neck and shoulder muscle activities at different screen height settings of the visual display terminal.

The effects of the VDT screen height on working posture and electromyographic (EMG) activities of the neck and shoulder muscles were determined in 10 healthy subjects. The subjects performed a mouse-driven interactive task at the screen height settings of 80, 100 and 120 cm. Changes in body positions were measured by video image and frame analysis. Surface EMG recordings were done on the neck extensor muscle and the descending part of the trapezius muscle, both on the right side. The results of the postural analysis showed that at higher screen height settings, the neck became significantly more erect. The subjects also assumed a more backward-leaning trunk position at higher screen heights. The EMG activities of the neck and shoulder muscles were related to the neck angle and trunk inclination, respectively. A more flexed neck produced significantly higher neck extensor muscle activities. A backward-leaning trunk was also noted to decrease trapezius muscle activity in some subjects.

Adult↗
PubMed1996

[Concentration and fiber size of asbestos in lungs of residents living close to the serpentinite area].

Concentrations and fiber size distribution of asbestos in the lung tissue of 16 ( 8 males and 8 females) rural residents living close to the serpentinite area (neighboring group) were analyzed by low temperature ashing-analytical transmission electron microscopy, and were compared with those of 5 (5 males) rural and 53 (34 males and 19 females) urban residents living away from the serpentinite area (reference group). The results are as follows; 1. Pulmonary asbestos fibers were found in all rural residents. The types of asbestos fibers were chrysotile, amosite, crocidolite, actinolite, and tremolite. These findings were very similar to results for urban residents. 2. In males, the geometric mean concentration of actinolite-tremolite in the neighboring group (1.78 x 10(6) fibers/g dry lung) was significantly higher than those in the reference groups (rural: 0.32 x 10(6), urban: 0.31 x 10(6), p < 0.01). In females also, the geometric mean concentration of actinolite-tremolite in the neighboring group (0.59 x 10(6)) was significantly higher than that in the reference group (urban: 0.16 x 10(6), p < 0.05). There were no significant differences in the actinolite-tremolite concentrations between males and females in the neighboring group. 3. The serpentinite in this area have contained not only chrysotile but also both actinolite and tremolite. There was no significant relationship between actinolite-tremolite concentration and occupational history in the neighboring group. It is possible that actinolite and tremolite are liberated from the serpentinite to the atmosphere and inhaled by the residents near the serpentinite area which increases pulmonary actinolite-tremolite concentration. 4. The difference in the geometric mean concentration of chrysotile between the neighboring and the reference group was not statistically significant for both sexes, possibly due to the poor durability of chrysotile in the lung tissue. 5. There were no significant differences in the geometric mean of fiber length and diameter of actinolite-tremolite between the neighboring and the reference group.

Adolescent↗
PubMed1995

Toluene induces behavioral activation without affecting striatal dopamine metabolism in the rat: behavioral and microdialysis studies.

We examined the effects of toluene on the release of dopamine (DA) and its metabolites in rat striatum using microdialysis. Intraperitoneal injection of 800 mg/kg toluene significantly increased motor activity in rats, as did methamphetamine (MAP) (1 mg/kg). However, 800 mg/kg toluene did not affect the extracellular levels of DA, 3,4-dihydroxyphenylacetic acid, homovanillic acid, or 5-hydroxyindoleacetic acid. This is in contrast to MAP, which significantly increased extracellular DA and decreased the extracellular levels of its metabolites. These results suggest that toluene-induced behavioral augmentation may not be associated with alterations in DA or serotonin neurochemistry such as are associated with MAP-induced behavioral augmentation.

Animals↗
PubMed1994

Asbestos and nonasbestos fiber content in lung tissue of Japanese patients with malignant mesothelioma.

BACKGROUND: Pulmonary fiber content of both asbestos and nonasbestos types were evaluated in Japanese patients with malignant pleural mesotheliomas. METHODS: Pulmonary fiber content was analyzed in 16 patients and 16 case-matched control subjects by transmission electron microscopy with energy-dispersive X-ray analysis using a low-temperature ashing procedure. RESULTS: The geometric mean content of total asbestos was significantly higher in the patients (22.0 x 10(6) fibers/g dry lung) than in the control subjects (2.24 x 10(6) fibers/g dry lung) (P < 0.01). When the asbestos content was analyzed by fiber type, the geometric means were also consistently and significantly higher among the patients compared with the control subjects (P < 0.01). Results were as follows: (1) amosite: patients 3.94 times 10(6) versus control subjects 0.23 x 10(6); (2) crocidolite: patients 3.56 times 10(6) versus control subjects 0.35 times 10(6); (3) total amphiboles: patients 16.0 times 10(6) versus control subjects 0.77 times 10(6); and (4) chrysotile: patients 3.76 times 10(6) versus control subjects 1.01 times 10(6). However, when individual total asbestos content was considered, 7 of the 16 patients (44%) had levels lower than the highest value noted among the control subjects. Pulmonary fiber content of patients and control subjects also revealed the presence of nonasbestos fibers. The geometric mean of nonasbestos fibers was significantly higher in the patients (87.3 x 10(6)) than in control subjects (33.8 x 10(6)) (P < 0.01). The major type of nonasbestos fibers in both groups was aluminum silicates. The mean of ratios of nonasbestos fiber contents to total asbestos contents in the patients and control subjects was 7.0 and 17.3, respectively. CONCLUSIONS: The results were mainly in agreement with the findings of earlier investigations, but fiber content of both chrysotile and nonasbestos fiber as well as those of amphibole asbestos were significantly higher in the patients than in the control subjects.

Adult↗
PubMed1994

[Medical examination of workers exposed to lead in the Philippines].

The medical examination of workers exposed to lead was conducted as part of the activity of the Occupational Safety and Health Center (OSHC) Project in the Philippines (JICA Project). The subjects of the medical examination were 21 male and 193 female workers of a semi-conductor plant (abbreviated A company), 59 male and 6 female workers of a refrigerator manufacture (B company); 199 male workers of a storage battery manufacturer (C company); and 107 male and 6 female workers of a lead smelter (D company). Among the examination items were questions regarding occupational history, subjective complaints and objective signs, determination of blood lead levels, urine delta aminolevulinic acid levels, and examination for anemia. The survey was conducted from June to September in 1990. The following results were obtained: 1) The mean age of the workers ranged from 21.8 to 33.8 years. Those of companies A and B were younger than those of companies C and D. The mean employment duration of males at C company was 10.7 yr, and the longest among the four companies. That in both sexes for A company was 1.8 yr and the shortest among the above mentioned companies. 2) The blood lead geometric mean levels of companies C and D showed the highest concentration. The level in males of C company was 64.5 micrograms/dl and that of D company was 80.8 micrograms/dl. The level in females of A company was 9.9 micrograms/dl and the lowest in concentration. The urine delta aminolevulinic acid geometric mean levels were less than 6.0 mg/l in the four companies. There was no company having hemoglobin mean values less than 14.0 g/dl in males or less than 12.0 g/dl in females. 3) The proportion of blood lead levels of 60 micrograms/dl or more was 67.3% in males of C company, and 89.7% in males and 16.7% in females of D company. The proportion of urine delta aminolevulinic acid levels of 6 mg/l or more was 1.0% in females of A company, 20.1% in males of C company, and 43.0% in males of D company. The proportion of hemoglobin values less than 14.0 g/dl in males or less than 12.0 g/dl in females was 4.7% in females of A company, 1.7% in males of B company, 3.5% in males of C company, and 12.1% in males of D company.(ABSTRACT TRUNCATED AT 400 WORDS)

Adult↗
PubMed1993

Urinary metabolite levels and symptoms in Filipino workers using organic solvents.

To compare symptoms with urinary metabolite levels, 900 workers from 7 organic solvent-using industries were studied. Urinary metabolites were determined using a high performance liquid chromatograph. Urinary hippuric acid concentrations exceeding the reference value (2.5 g/g creatinine) were found in 78 (8.7%) workers. However, only 3 (0.3%) and 1 (0.1%) of the participants exceeded the reference value for mandelic (0.8 g/g creatinine) and total methylhippuric acid (1.5 g/g creatinine), respectively. The sum of the values of the ratio of measured urinary metabolite concentration to the corresponding ACGIH's biological exposure indices (BEI) [(HA/BEI of HA + MHA/BEI of MHA + MA/BEI of MA)] exceeded 1.0 in 166 (18.4%) workers. Majority of them were from the footwear manufacturing industry (63/129 or 49.2%). Questionnaire interviews were also administered to determine the prevalence of symptoms while at work (acute symptoms) or within the past 6 months (chronic symptoms). Urinary metabolite levels of individual and mixed solvents were compared with the symptoms of all workers. Analysis using Spearman's rank correlation showed in workers whose urinary hippuric acid exceeded 3.75 g/g creatine (1.5 x BEI), significant correlation between their hippuric acid levels and subjective complaints. Workers whose sum of the values of the ratio of measured urinary metabolite concentration to corresponding BEI exceeded 1.5 were selected and comparing this level with their symptoms, significant correlation was also noted in some complaints.

Biomarkers↗
PubMed1993

Modification of metabolism and neurotoxicity of hexane by co-exposure of toluene.

The effects of co-exposure of hexane and toluene were investigated in field surveys and animal experiments. One field survey suggested that increase of hexane content in adhesives might have caused an outbreak of polyneuropathy in a vinyl sandal manufacture in Japan. The animal experiments proved that co-exposure of hexane and toluene decrease hexane neurotoxicity and urinary excretion of hexane metabolites in rats. The results also suggested that toluene might inhibit metabolism of hexane. Another recent field survey indicated that the ratio of urinary 2,5-hexanedione to hexane exposure in the workers co-exposed to hexane and toluene decreased in parallel with in more crease of toluene concentration. The results indicated that urinary excretion of 2,5-hexanedione could be depressed by co-exposure of toluene even in the workers exposed to relatively low concentrations. These above-mentioned results suggest that co-exposure of hexane and toluene could inhibit hexane metabolism and decrease hexane neurotoxicity in both experimental animals and workers. Although metabolism of hexane could be easily modified by toluene or other solvents and might not be a good indicator for hexane exposure in mixed exposure, urinary 2,5-hexanedione might be a good indicator for neurotoxicity of hexane even in mixed exposure.

Adhesives↗
PubMed1993

[Inhaled nonasbestos fibers; pulmonary concentrations and fiber size in autopsied urban residents].

Nonasbestos fiber concentrations and fiber size distribution in autopsied lung tissues of 53 urban residents (34 males and 19 females) were analyzed by low temperature ashing-analytical transmission electron microscopy. The following findings were obtained. 1. Pulmonary nonasbestos fibers were found in all samples. Fiber consisting mainly of aluminum and silicon represented 80.0% of the total nonasbestos fibers observed. 2. The geometric mean concentration of nonasbestos fiber was significantly higher in males (47.6 x 10(6) fibers/g dry lung) than in females (18.2 x 10(6) fibers/g dry lung) (p < 0.05). This may be caused by differences in occupational exposure to nonasbestos fibers between males and females. 3. In females, the geometric mean concentration of nonasbestos fibers of smokers (34.6 x 10(6) fibers/g dry lung) was higher than that of nonsmokers (11.4 x 10(6) fibers/g dry lung), while, in males, the geometric mean concentration of nonasbestos fibers was lower in smokers (53.9 x 10(6) fibers/g dry lung) than in nonsmokers (89.1 x 10(6) fibers/g dry lung). These differences were not statistically significant. 4. The mean value for the ratio of nonasbestos fiber to asbestos concentration which ranged from 2.3 to 268 in males, and from 1.3 to 52.0 in females, was 30.1 and 20.2 respectively. 5. The mean length, diameter and aspect ratio for nonasbestos fibers were 1.01 microns, 0.103 microns and 11.3, respectively. Although nonasbestos fibers were almost equal in length to asbestos, the mean diameter of nonasbestos fibers was almost one-half of that of asbestos. Most of nonasbestos fibers (99.0%) were too small to be visible by light microscopy.

Adult↗
PubMed1992

Absence of blue-yellow color vision loss among workers exposed to toluene or tetrachloroethylene, mostly at levels below occupational exposure limits.

Possible color vision loss was examined with Lanthony's new color test and Ishihara's color vision test in 261 solvent workers and 120 controls (48 men and 72 women). The solvent workers were exposed to either predominantly toluene [46 ppm as geometric mean (GM); 63 men and 111 women], tetrachloroethylene alone (13 ppm; 30 men and 34 women), or a mixture (14 men and 9 women) of tetrachloroethylene (12 ppm) and trichloroethylene (7 ppm). The only instances of color vision loss that were detected in either the exposed workers or the controls were six cases of red-green loss (all in men). These six cases of red-green loss showed an unbiased distribution between the exposed workers and the nonexposed controls.

Adult↗
PubMed1992

Dose dependent effects of chronic exposure to toluene on neuronal and glial cell marker proteins in the central nervous system of rats.

The dose dependent effects of chronic exposure to toluene on the neuronal marker proteins (gamma-enolase, calbindin-D28k) and glial cell marker proteins (alpha-enolase, creatine kinase-B, and beta-S100 protein) were investigated in the central nervous system (CNS) of rats. Three groups of animals were exposed to 100 ppm, 300 ppm, or 1000 ppm toluene vapour eight hours a day, six days a week for 16 weeks. The contents of the marker substances were determined with enzyme immunoassays. A significant increase in the three glial cell marker proteins was noted in the cerebellum after exposure to 100 ppm toluene; a more pronounced increase occurred at the higher toluene concentrations. beta-S100 protein also exhibited a dose dependent increase in the brainstem and spinal cord. On the other hand, the two neuronal cell markers did not show a quantitative decrease in the CNS. This means that the development of gliosis, rather than neurone death, is induced by chronic exposure to toluene. The significant biochemical changes induced around the threshold limit value and the concentration dependent alterations suggest that these nerve specific marker proteins may be used to evaluate solvent related damage to the CNS.

Animals↗
PubMed1991

Determination of urinary 2,5-hexanedione concentration by an improved analytical method as an index of exposure to n-hexane.

2,5-Hexanedione is a main metabolite of n-hexane and is considered as the cause of n-hexane polyneuropathy. Therefore, it is useful to measure 2,5-hexanedione for biological monitoring of exposure to n-hexane. The analytical methods existing for n-hexane metabolites, however, were controversial and not established enough. Hence, a simple and precise method for determination of urinary 2,5-hexanedione has been developed. Five ml of urine was acidified to pH 0.5 with concentrated hydrochloric acid and heated for 30 minutes at 90-100 degrees C. After cooling in water, sodium chloride and dichloromethane containing internal standard were added. The sample was shaken and centrifuged. 2,5-Hexanedione concentration in an aliquot of dichloromethane extract was quantified by gas chromatography using a widebore column (DB-1701). Urinary concentration of 2,5-hexanedione showed a good correlation with exposure to n-hexane (n = 50, r = 0.973, p less than 0.001). This method is simple and precise for analysis of urinary 2,5-hexanedione as an index of exposure to n-hexane.

Adhesives↗