[Variations of required nutrients for human tubercle bacilli. Growing in the culture media without glycerin. (2)].
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Biomedical subjects
N Hirano
Publications and source records attributed to N Hirano.
The effect of alcohol injection on suckling-induced PRL and alpha-MSH release in lactating rats.
The effects of acute treatment with alcohol on prolactin (PRL) surge and immunohistochemical features of PRL cells and alpha-melanocyte-stimulating hormone (alpha-MSH) cells in lactating rats were investigated. In light microscopic observations, one could see numerous large, lightly immunoreactive PRL cells in the pars distalis of control lactating rats. These cells revealed well-developed Golgi zones, prominently dilated ER, and a few granules in the electron microscopic observations. The alcohol-treatment depressed the occurrence of such "active" cells. In addition, following resuckling, the immunoreactivity to alpha-MSH of the intermediate lobe was decreased in light microscopic observations and a release of alpha-MSH granules in cells of the intermediate lobe was detected in electron microscopic observations. These changes were prevented by the injection of alcohol. These results indicate that suckling induces an increase of the release of PRL granules from PRL-cells in the pars distalis and alpha-MSH granules from the cells in the intermediate lobe which are correlated with the appearance of the PRL surge. Further, acute administration of alcohol to lactating rats prevents both changes and consequently inhibits the suckling-induced PRL surge.
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Reversibility of organic anion-induced cholestasis: association with compensatory hypersecretion of biliary phospholipid and protein in the dog.
The effect of a concomitant infusion of organic anions, structurally related phthaleins, on bile flow was studied in anaesthetized dogs. A combination of rose bengal and sulfobromophthalein was found to uniquely and synergistically produce an acute, reversible form of intrahepatic cholestasis (< 10% of control level). This phenomenon was not observed with the administration of those individual organic anions at concentrations previously associated with the induction of intrahepatic cholestasis. The infusion of either a micelle forming bile salt, sodium taurocholate, or a non-micelle forming bile salt, sodium dehydrocholate, rapidly reversed the intrahepatic cholestasis (within 20 min after bile salt infusion). During the choleretic phase immediately following the bile salt infusion, a transient but marked hypersecretion, a disproportionately increased output in relation to that of bile acids, of biliary phospholipid (176% of control level by taurocholate and 138% of control level by dehydrocholate), and an even more striking amount of biliary protein hypersecretion were observed (392% of control level by taurocholate and 357% of control level by dehydrocholate). Although the significance of these new post-cholestatic observations requires clarification, it is suggested that the intrahepatic cholestasis induced by organic anions reflects a reversible defect in the mechanism(s) involved in transcellular transport.
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