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Biomedical subjects

N Hartman

Publications and source records attributed to N Hartman.

26 records · Page 2Linked to original sources
PubMed1976

Change in Ig expression of a human lymphoblastoid line during long-term culture.

A human lymphoblastoid line, in continuous culture for over 3 years, showed changes in Ig expression with time. The following pattern of events was suggested by studies of Ig synthesis and secretion performed at intervals. In a mass culture containing a heterogeneous cell population, Ig expression may shift because of selection acting on the different phenotypes. In human lymphoblastoid lines there may be a tendency for selection to favor IgMchi-producing cells. In clones, including those that synthesize multiple H chains, substantial determinism for Ig expression is present.

Cell Line↗
PubMed1975

A glycoprotein associated with the membrane fraction of human B but not T lymphocytes.

A method is described which employs differential centrifugation and sucrose density gradient centrifugation to isolate a membrane fraction from human lymphocytes. Membrane preparations from long-term human cultured B- and T-lymphoid lines, peripheral blood lymphocytes, tonsillar lymphocytes, and thymocytes were analyzed on 0.5% sodium dodecyl sulfate-7.5% polyacrylamide gels stained for protein and carbohydrate. The most important finding was a major glycoprotein of approximately 30,000 daltons associated with the membrane preparations from B lymphocytes. T-lymphocyte preparations did not contain readily detectable amounts of this membrane-associated component. The T-cell lymphoid line MOLT-4 was unique in that it had a narrow protein band at approximately 30,000 daltons which did not contain carbohydrate.

Antigens↗
PubMedDate not supplied

Resolution of migraine following bromocriptine treatment of a prolactinoma (pituitary microadenoma).

A 39-year-old male physician with a 27-year history of chronic severe migraine had a prolactin-secreting pituitary microadenoma diagnosed as an incidental finding following an automobile accident. Treatment of the prolactinoma with bromocriptine provided complete and lasting resolution of the migraine as well, suggesting a possible etiologic relationship between these two prevalent conditions, and the possibility of treating at least some cases of migraine with bromocriptine.

Adult↗