A modified antigen--antibody crossed electrophoresis characterizing the specificity and titre of human precipiting against Candida albicans.
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Animals↗
Biomedical subjects
N H Axelsen
Publications and source records attributed to N H Axelsen.
A low-dose immunization schedule for the production of ALG in rabbits.
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Animals↗
Antigen-antibody crossed electrophoresis (Laurell) applied to the study of the antigenic structure of Candida albicans.
By means of the antigen-antibody crossed electrophoresis procedure of Laurell, 68 antigens were demonstrated in Candida albicans. This is about four times the number of antigens described earlier by means of classical immunoelectrophoresis. The procedures for obtaining this result are described, including the preparation of antigen, the immunization of rabbits, and the method of N. M. G. Harboe for the production of purified and concentrated rabbit antibodies suitable for quantitative immunoelectrophoresis. The immunoplates were stained by means of the sensitive Coomassie brilliant blue R. The various quantitative immunoelectrophoretic methods offer considerable possibilities for qualitative and quantitative characterization of antigens, even in complex mixtures, and are therefore well suited for the investigation of microbial antigens.
Animals↗
Human precipitins against a micro-organism (Candida albicans) demonstrated by means of quantitative immunoelectrophoresis.
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Autoimmune Diseases↗
Post-gamma protein in urinary protein. Occurrence, clinical significance and probable identity with muramidase.
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Adult↗
Purified flagella from Treponema phagedenis biotype Reiter does not induce protective immunity against experimental syphilis in rabbits.
Thirteen rabbits were immunized three times at weekly intervals with 50 micrograms of flagella purified from Treponema phagedenis biotype Reiter. Fourteen rabbits were inoculated in the same way with a placebo preparation. Rabbits immunized with the flagella developed an immune response to the flagella but showed no statistically significant prolongation of incubation time or diminution of lesion severity when challenged intradermally with 4 X 10(3) Treponema pallidum organisms.
Animals↗