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Biomedical subjects

N Goto

Publications and source records attributed to N Goto.

At least 307 records · Page 17Linked to original sources
PubMed1987

Overproduction of gene products by the super-high-copy-number plasmid pNR333.

The plasmid pNR333 is a kanamycin-resistant, deletion derivative of pNR113 with an extremely high copy number in Escherichia coli and in Proteus mirabilis. In order to determine the usefulness of pNR333 as a replication gene of vector, the genes encoding chloramphenicol acetyl-transferase (CAT) and beta-galactosidase (beta-gal) were cloned individually into both pNR333 and other low-copy-number plasmids. The expression of the cloned genes was compared by measuring the specific activity of each enzyme and the amounts of the proteins produced. A hybrid plasmid pNR333-cat expressed 53 times as much activity of CAT as the low-copy plasmid S-a which had a copy number of four. The lacZ gene cloned in pNR333 produced 17 times as much beta-gal as in the low-copy-number plasmid pNR1150. These results suggest that pNR333 is a useful vector plasmid for producing a large amount of polypeptides in E. coli hosts.

Acetyltransferases↗
PubMed1987

Identification of the DNA sequence required for transposition immunity of the gamma delta sequence.

A plasmid containing the transposon gamma delta sequence was immune to further insertion of gamma delta (transposition immunity). Plasmids carrying a fragment containing either 0.2 kilobase pairs of the gamma end or 0.4 kilobase pairs of the delta end of the gamma delta sequence were immune, and other parts of the gamma delta sequence did not confer immunity. The terminal 38-base-pair (bp) sequence of the delta end of the gamma delta was sufficient to confer immunity, the 38-bp sequence of the gamma end conferred only moderate immunity, and the terminal 35-bp sequence, which was completely identical at both the gamma and delta ends, was insufficient to confer immunity.

Base Sequence↗
PubMed1987

An avian transforming retrovirus isolated from a nephroblastoma that carries the fos gene as the oncogene.

A new avian transforming retrovirus, NK24, was isolated from a chicken with a nephroblastoma. This transforming virus induced fibrosarcomas with osteogenic cell proliferation and nephroblastomas in vivo and transformed fibroblast cells in vitro. From extracts of NK24-transformed cells, anti-gag serum immunoprecipitated a 100-kilodalton nonglycosylated protein with no detectable protein kinase activity. An NK24 provirus present in infected quail cells was molecularly cloned and subjected to nucleotide sequence analysis. The genome of NK24 was 5.3 kilobases long and had a 1,126-base-pair sequence of cellular origin in place of a viral sequence of avian leukosis virus containing the 3' half of the gag gene and the 5' half of the pol gene. Although the entire env gene was retained, it appeared to be inactive, possibly owing to the loss of function of its splice acceptor site as a result of a second deletion of 1,598 bases in the 3' half of the pol gene that extended to the acceptor site. Nucleotide sequence analysis revealed that the NK24 virus contained the fos gene, previously identified as the oncogene of FBJ and FBR murine osteosarcoma viruses. Unlike the v-fos gene products of FBJ and FBR, which suffer a structural alteration at their carboxyl termini, the NK24 v-fos gene product seemed to have the same carboxyl-terminal structure as the chicken c-fos gene product. A comparison of the structures of the products of the NK24 v-fos and mouse c-fos genes suggested that the fos gene product consists of highly conserved regions and relatively divergent regions.

Amino Acid Sequence↗
PubMed1987

Morphometrical observations on the mandible of five strains of rabbits and strain identification using mandible measurements.

In order to clarify the relationships between five strains of rabbits and to identify the strains, principal component and discriminant analyses were carried out using 12 mandibular measurements of three inbred strains (JW-NIBS/Y (JW/Y), NW-NIBS/Y (NW/Y) and Dutch-NIBS/Y (D/Y)) and two outbred strains (JW-NIBS (JW) and NW-NIBS (NW] which were maintained at the Nippon Institute for Biological Science. The results obtained were as follows. (1) Principal component analysis revealed that in the males the mandible of JW was the largest but with considerable variation. D/Y was the smallest of all strains examined. The mandibles of NW and NW/Y were similar to the JW mandibles but had a shape which was shorter and higher. In the females the mandible of NW was the largest of all strains and, as in the males, D/Y was the smallest and JW varied markedly. (2) Discriminant analysis showed the probability of erroneous discrimination to be 14.8% (34/229) when the inbred and outbred strains were combined. In both sexes erroneous discrimination mostly occurred between NW and NW/Y, which have the same origin, and between JW and NW, which have a common ancestor. However, when the inbreds and outbreds were identified separately by discriminant analysis, the probability of erroneous discrimination was low in both cases (4.5% (5/112) and 7.7% (9/117) respectively). These results indicate that strain differences are found in the size and shape of rabbit mandibles and that mandible analysis is effective for strain identification of laboratory rabbits.

Animals↗
PubMed1987

The Mexican hairless dog, its morphology and inheritance.

Some morphological characteristics of the Mexican hairless dog and their inheritance were investigated. The dogs examined were hairless and had defective teeth. From the results of mating experiments, an autosomal dominant semi-lethal gene was considered to be responsible for the hairlessness accompanied by defective teeth in the dog. The possible usefulness of the dog was also discussed.

Animals↗
PubMed1987

[Discriminant analysis of hematological and serum biochemical values in cynomolgus monkey (Macaca fascicularis) bred and reared under the indoor individually-caged conditions].

The data on hematological and serum biochemical properties of laboratory-bred cynomolgus monkeys (Macaca fascicularis) at different ages were analyzed by discriminant analysis. All the animals had been bred and reared under uniform environmental conditions at Tsukuba Primate Center for Medical Science, N.I.H., Japan. The items used were as follows: red blood cell count (RBC), hematocrit value (Ht), hemoglobin concentration (Hb), mean corpuscular volume (MCV), white blood cell count (WBC), glutamic oxaloacetic transaminase activity (GOT), glutamic pyruvic transaminase activity (GPT), total protein concentration (TP), albumin concentration (ALB), albumin-globulin ratio (A/G), blood urea nitrogen (BUN), glucose concentration (GLU), total cholesterol concentration (TCHO), free cholesterol concentration (FCHO), triglyceride concentration (TG) and alkaline phosphatase activity (ALP). In total, 1086 animals in 10 age groups were examined. Data analyses were done with respect to the difference of sex. Discrimination was possible by Mahalanobis' generalized distance between centroids of groups. In canonical discriminant analysis (discriminant analysis with reduction of dimensionality), age was highly correlated to the value of the first canonical variate. From the approximate relative value of the eigenvector of the first canonical variate, the most discriminant variables are WBC, TP, ALB, A/G, TCHO, FCHO, TG, and ALP. It can be concluded that periodic measurement of these 8 parameters is necessary and sufficient to monitor the physiological conditions of growing monkeys.

Age Factors↗
PubMed1987

Discriminative staining methods for the nervous system: luxol fast blue--periodic acid-Schiff--hematoxylin triple stain and subsidiary staining methods.

This paper describes a new series of staining methods which can discriminatively demonstrate every structure of the nervous system, including axons and capillaries, in animal and human materials. Methods described in this paper consist of one primary stain, luxol fast blue-periodic acid Schiff-hematoxylin (LPH) and six different subsidiary staining methods. The LPH triple stain can precisely differentiate the following structures: neurons (Nissl bodies, cytoplasm, nuclear membrane and nucleolus), various kinds of nuclei (glia, ependyma, endothelium, leucocyte, connective tissue, etc.), myelin sheaths, neuronal processes (axons and dendrites), reacted glial cell bodies (protoplasmic astrocytes, foamy cells, etc.), blood vessels (arteries, veins and capillaries), meninges, intervening connective tissue, erythrocytes, lipofuscin granules, amyloid bodies, and others. Subsidiary staining methods are also described briefly. Applications are discussed in the context of staining technology and neuromorphological research.

Animals↗
PubMed1987

Postinflammatory remyelination in the spinal cord of mice infected with mouse hepatitis virus, JHM strain.

The spinal cords in mice surviving acute infection for 30 days following lumbar inoculation with mouse hepatitis virus, JHM strain, were examined for virus growth and pathologic changes by light and electron microscopy. Virus titer reached a maximum level on day 6 postinoculation (p.i.) and became negative on day 10 p.i. On days 5 to 10 p.i. there were severe disruption of myelin sheath and marked phagocytosis of myelin by macrophages. Viral particles were seen in degenerating glial cells. On days 15 to 20 p.i. proliferation of oligodendrocytes was noticed in association with thinly myelinated axons with decreased number of macrophages. On days 25 to 30 p.i. affected axons were mostly remyelinated by oligodendrocytes and the interaxonal space was packed with the processes of fibrous astrocytes containing myelin debris and lipid droplets. In the subpial areas of more widespread lesions some axons were remyelinated by Schwann cells.

Animals↗
PubMed1987

Effect of famotidine on hepatic hemodynamics and peptic ulcer.

The acute effects of histamine H2 receptor antagonist, famotidine, on hepatic hemodynamics were studied in six normal volunteers and eight patients with chronic liver disease, and its chronic effects on peptic ulcer, portal hemodynamics, and hepatic function were studied in 20 patients with chronic liver disease and peptic ulcer (16 with gastric ulcer, four with duodenal ulcer). Infusion of 20 mg famotidine did not reduce hepatic blood flow and portal blood flow in normal subjects, nor did it reduce cardiac output, the gradient between wedged hepatic vein pressure and free hepatic vein pressure, hepatic blood flow, and portal blood flow in patients with chronic liver disease. An oral dose of 20 mg famotidine twice daily for 2 months healed peptic ulcer in 19 of 20 patients (95%) with chronic liver disease and peptic ulcer, without any change in portal blood flow and hepatic function. Thus famotidine does not appear to alter hepatic hemodynamics and hepatic function in normal subjects and patients with chronic liver disease.

Adult↗