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Biomedical subjects

N Goldman

Publications and source records attributed to N Goldman.

At least 91 records · Page 5Linked to original sources
PubMed1980

Adenovirus regulation of simian virus 40 macromolecular synthesis.

At low multiplicities of infection (1 to 5 PFU/cell), human adenoviruses grew inefficiently in monkey kidney cells as the result of a late transcriptional and/or translational defect. Through an unknown mechanism, coinfection with simian virus 40 (SV40) could overcome this block. Coincident with the onset of adenovirus DNA replication in coinfected monkey cells, however, there was a marked inhibition of SV40 DNA replication. Since this inhibition in SV40 DNA synthesis occurred in the presence of normal SV40 transcription and protein synthesis, we suggest that adenovirus competes with SV40 for molecules specifically required for DNA synthesis (e.g., SV40 T-antigen). At high multiplicities of adenovirus infection (50 PFU/cell), adenoviruses could grow efficiently in monkey kidney cells. In cells coinfected under these conditions, SV40 transcription was generally inhibited, as shown by a decrease in nuclear and cytoplasmic SV40 mRNA.

Adenoviruses, Human↗
PubMed1980

New chimeric splice junction in adenovirus type 2-simian virus 40 hybrid viral mRNA.

We have examined hybrid viral RNAs synthesized in both human and monkey cells infected by three nondefective adenovirus type 2 (Ad2)-simian virus 40 (SV40) hybrid viruses; Ad2+ND1, Ad2+ND2, and Ad2+ND4. Most of the hybrid viral RNA molecules appeared to be initiated within adenoviral sequences, but were polyadenylated on their 3' end at the early SV40 mRNA polyadenylation site. The Ad2+ND4 stock of virus was not homogeneous, but consisted of two principle populations of viral DNA. Both populations contained a segment of SV40 DNA extending from the SV40 map positions 0.63 to 0.11 in a left-to-right orientation at adenovirus map position 0.82. One population contained an intact SV40 segment, whereas the other (representing 80 to 85% of the population) has a 500-base pair deletion mapping from approximately 0.60 to 0.50 SV40 map units. This deletion encompassed the SV40 DNA segment which encodes the early SV40 splice sites. Cells infected by the mixed Ad2+ND4 population induced the synthesis of at least three major SV40 RNA species among the hybrid viral transcripts. The most abundant of these hybrid mRNA's appeared only late in the lytic cycle, after the onset of viral DNA replication. It contained an RNA splice junction which extended from a donor (5') nucleotide within the adenoviral RNA sequences to an acceptor (3') splice site within the early region of SV40 at 0.46 SV40 map units. This SV40 acceptor splice site was remarkable in that its use has not been detected in the spliced viral mRNA's of SV40-infected or -transformed cells.

Adenoviridae↗
PubMed1978

Estimating the intrinsic rate of increase of a population from the average numbers of younger and older sisters.

Based on stable population theory, a mathematical relationship is developed between the intrinsic rate of increase (r) of a population and the ratio (Z) of the average number of younger sisters ever born to the average number of older sisters ever born, for a random sample of women in the population. This mathematical formula is then converted into a technique for estimating r from data on numbers of sisters. The extent to which the technique may be generalizable to actual populations is discussed.

Birth Rate↗
PubMedDate not supplied

Elevated P-selectin on platelets in depression: response to bupropion.

Increased platelet activation has been suggested as a possible reason for the increased vulnerability of depressed patients to ischemic heart disease (IHD). Translocation of P-selectin, an integral alpha-granule membrane protein, to the platelet surface is a measure of platelet activation. Herein, western blots of platelet plasma membranes containing P-selectin were quantified in patients with major depression (n=19; mean age=39 +/- 2 years) and healthy comparison subjects (n=17; mean age=36 +/- 2 years). None evidenced clinical signs of IHD, and only two patients had a lifestyle IHD risk factor (smoking). Blood was obtained from all 19 depressed patients before treatment, and 15 returned after 6-8 weeks of open-label bupropion treatment. Bupropion was chosen as the antidepressant because it did not elevate plasma norepinephrine or serotonin, endogenous agonists that can induce platelet degranulation. Western blotting revealed more P-selectin immunoreactivity (75 kD band) in depressed patients compared to healthy controls (P=0.003). After bupropion treatment, P-selectin remained high in depressed patients. beta3-Integrin, a reference plasma membrane protein that does not translocate during activation, was of equivalent density in depressed patients and healthy control subjects, and was unchanged after treatment with bupropion. P-Selectin failed to correlate with severity of illness based on the Hamilton Depression scale, or with the post-treatment plasma concentration of bupropion. The results suggest an elevation in P-selectin on platelet plasma membranes might be a trait marker for depression.

Adolescent↗
PubMedDate not supplied

Effect of bupropion on immunodensity of putative imidazoline receptors on platelets of depressed patients.

A substantial number of studies have demonstrated increased imidazoline receptors (I1 binding sites) on platelets of depressed patients and downregulation following antidepressant treatments. Herein, imidazoline receptor binding protein (IRBP) antiserum was used to quantify imidazoline receptors on platelets of depressed patients before and after treatment with the atypical aminoketone antidepressant, bupropion. Western blots revealed an increase in IRBP-immunodensity (p = 0.01, two-tailed) in a 33 kDa protein band in untreated depressed patients (n = 21) as compared with controls (n = 17). This band has been positively correlated with I1 binding sites on platelets. Following 6 weeks' treatment with bupropion, IRBP-immunodensity was downregulated in depressed patients (p = 0.03, paired t-test); predominantly in responders (p = 0.005). Patients non-responsive to bupropion (n = 5) were significantly different from responders (p = 0.05) by exhibiting no elevation in IRBP-immunodensity at pre-treatment and no downregulation of the 33 kDa band after treatment. IRBP-immunodensity was negatively correlated (r = -0.79, p = 0.01) with plasma concentrations of bupropion and its metabolites at week-4 of BUP treatment. Thus, a 33-kDa IRBP on platelet plasma membranes is elevated in depression and normalized in responders to bupropion.

Adolescent↗
PubMedDate not supplied

Fecundability and husband's age.

The effect of husband's age on the probability of conception is evaluated from World Fertility Survey data in five developing countries: the Ivory Coast, Ghana Kenya, the Sudan, and Syria. Proportional hazards models, which include wife's age, husband's age, marriage duration, union type, and post-partum exposure as covariates, are used to describe the monthly conception rate for second and higher-order birth intervals in which no contraception was used. With the exception of Syria, the resulting models indicate that the effects of male age are generally small in relation to the influences of marital duration and the age of the woman.

Adult↗
PubMedDate not supplied

An evaluation of survey data on birthweight and prematurity status.

The 1986 experimental Demographic and Health Survey in the Dominican Republic was one of the first retrospective, population-based surveys of a developing country to collect information on prematurity status as well as birthweight. We show that the relationships among birthweight, prematurity status, and infant mortality are consistent with corresponding patterns observed in other Latin American and Caribbean countries and in developed nations. Two notable irregularities in the birthweight data are the atypically high proportion of infants weighing 4,000 grams or more, and the high mortality rates among infants with missing birthweight. Problems with the prematurity status data include an unexpectedly low proportion of premature births, an unusually small proportion of low birthweight explained by premature births, and an extremely high neonatal death rate among premature compared to term infants. Inclusion of an explicit definition of "premature" may help avert similar problems in future health surveys.

Birth Weight↗
PubMedDate not supplied

Exchange of life.

Explore the source record for details and available documents.

Female↗
PubMedDate not supplied

[A comparison of complete and truncated birth histories to measure fertility and child mortality].

"During the latter part of 1986, national probability sample surveys of women of reproductive ages were carried out in... Peru and the Dominican Republic. These surveys were made as part of the Demographic Health Surveys project (DHS). In each country, one survey was conducted with the standard core questionnaire developed for DHS; the other survey was based on an experimental questionnaire. The major difference between the two questionnaires is the inclusion in the experimental one of a monthly calendar, which records pregnancies, contraceptive use, reasons for contraceptive discontinuation, breastfeeding, post-partum amenorrhea, post-partum abstinence, women's employment and place of residence for the period 1981-1986. This paper presents results from the first stage of the analysis of the Peruvian data: a comparison of basic characteristics of the two samples and an assessment of the completeness of reporting of recent births and infant and child deaths, i.e., a comparison of information in the truncated and full birth histories." (SUMMARY IN ENG)

Americas↗
PubMedDate not supplied

Collection of survey data on contraception: an evaluation of an experiment in Peru.

The objective of this analysis is to compare two different approaches to the collection of information on contraceptive use. The data for this comparison are derived from the 1986 Demographic and Health Surveys (DHS) in Peru. Approximately 7,500 women were interviewed with the standard DHS questionnaire, whereas about 5,000 women received an "experimental questionnaire." The major difference between the questionnaires is the inclusion of a six-year monthly calendar in the experimental questionnaire that records pregnancies, contraceptive use, and postpartum information, in contrast to the more common tabular format of the standard questionnaire. The analysis demonstrates that although reports of contraceptive knowledge, ever-use, and current use are relatively robust to the variations in questionnaire design, estimates of past use are dependent on the survey instrument. Several different comparisons indicate that reporting of information on contraceptive histories in the experimental questionnaire is superior to that in the standard one.

Contraception Behavior↗