Search PubMed⌕ Search

Biomedical subjects

N Gilboa-Garber

Publications and source records attributed to N Gilboa-Garber.

60 records · Page 4Linked to original sources
PubMedDate not supplied

Adoptive transfer of resistance to Pseudomonas aeruginosa infection by splenocytes and bone marrow cells from BALB/c mice immunized by Pseudomonas aeruginosa lectin preparations.

BALB/c mice immunized by intraperitoneal injection of purified Pseudomonas aeruginosa lectin preparations are fully protected against a lethal dose of the live bacteria. Intraperitoneal inoculation of splenocytes or bone marrow cells obtained from actively immunized mice into naive syngeneic mice was shown to significantly increase their resistance to P. aeruginosa infection. A similar transfer of splenocytes or bone marrow cells from untreated control mice did not provide protection against a lethal Pseudomonas challenge. Administration of immunocompetent cells from immunized animals to naive mice by the i.v. route was less efficient than the i.p. inoculation, probably due to different homing of the cells. Only the i.p. injection concentrated the immune cells to the site of infection.

Animals↗
PubMedDate not supplied

'Subinhibitory' erythromycin represses production of Pseudomonas aeruginosa lectins, autoinducer and virulence factors.

Pseudomonas aeruginosa infection is preceded by selective adhesion of the bacteria to the host target cells via diverse adhesins, including lectins. This step enables maximal damage to the target host cells by the bacterially secreted injurious toxins and enzymes. The production of both lectins and many of the virulence factors is positively controlled by transcription activators including signaling autoinducers (N-acyl-L-homoserine lactones). We show in this communication that erythromycin at subminimal growth inhibitory concentrations simultaneously suppresses the production of P. aeruginosa hemagglutinins (including lectins), protease, hemolysin and homoserine lactone autoinducers. The antibiotic-treated bacteria also show reduced virulence to mice, endorsing clinical observations that indicate the efficiency of low-dose erythromycin treatment of persistent drug-resistant P. aeruginosa infections.

Adhesins, Bacterial↗
PubMedDate not supplied

Effect of lectins on tumorigenicity of AKR lymphoma cells of varying malignancy.

This study represents an attempt to characterize differences in the cell surfaces of cells of high - and low - malignancy by use of lectins. The effect of concanavalin A (con A), Phaseolus vulgaris lectin (PHA) and the galactophilic lectin of Pseudomonas aeruginosa (PA-I) on cell agglutination, viability and tumorigenicity of two variants of malignancy of AKR lymphoma was tested. Cell viability was not affected by the lectins. Agglutination of the two variants was similar with all lectins and was specifically inhibited by the respective sugars. However, the effect on tumorigenicity of the two variants differed markedly: it was strongly reduced by PHA in the less malignant variant, while the more malignant one was hardly affected. The effect of con A was, on the contrary, more accentuated on the high-malignancy variant. The effect of PA-I was in the same direction as con A, but reduction in oncogenicity of both variants was feeble.

Animals↗