Biomedical subjects
N Gardner
Publications and source records attributed to N Gardner.
My mama told me.
Explore the source record for details and available documents.
NADH dehydrogenase in Neurospora crassa contains myristic acid covalently linked to the ND5 subunit peptide.
The mitochondrial, proton-pumping NADH:ubiquinone oxidoreductase consists of at least 35 subunits whose synthesis is divided between the cytosol and mitochondria; this complex I catalyzes the first steps of mitochondrial electron transfer and proton translocation. Radiolabel from [(3)H]myristic acid was incorporated by Neurospora crassa into the mitochondrial-encoded, approximately 70 kDa ND5 subunit of NADH dehydrogenase, as shown by immunoprecipitation. This myristate apparently was linked to the peptide through an amide linkage at an invariant lysine residue (Lys546), based upon analyses of proteolysis products. The myristoylated lysine residue occurs in the predicted transmembrane helix 17 (residues 539-563) of ND5. A consensus amino acid sequence around this conserved residue exists in homologous subunits of NADH dehydrogenase. Cytochrome c oxidase subunit 1, in all prokaryotes and eukaryotes, contains this same consensus sequence surrounding the lysine which is myristoylated in N. crassa.
Doctors' complaints help forge insurance regulation. Payment due.
Arkansas doctors are banning to help draft a regulation that would require health maintenance organizations and insurance carriers to process health care claims within 45 calendar days after receipt of a claim. The proposed regulation would be administered by the Arkansas Department of Insurance. If additional information from a claimant is needed to process a claim, a carrier must notify a provider within 10 days of receipt of the claim. Once that information is received by the carrier, it has an additional 45 days to pay.
Foundation is a 'lifesaver' to many uninsured. Physicians give free care to state's working poor.
Explore the source record for details and available documents.
Regulation of human D1 dopamine receptor function and gene expression in SK-N-MC neuroblastoma cells.
SK-N-MC human neuroblastoma cells express functional D1, but not D5, dopaminergic receptors. Stimulating cells with dopamine or the D1-selective agonist, SKF R-38393, rapidly (t(1/2) = 1 h) resulted in > 95% attenuation of dopamine-mediated accumulation of cyclic AMP, without any change in D1 dopamine receptor levels. Prolonged (> 4 h) exposure of cells to dopamine attenuated D1 receptor levels to 45-50% of control (t(1/2) = 8 h) and was accompanied by a loss of high-affinity binding sites. At the molecular level, the expression of D1 receptor messenger RNA was bimodal: an initial increase (by approximately 60%) of receptor messenger RNA within 2 h of treatment of cells with dopamine was followed by a decline to 50% below control messenger RNA levels. Low concentrations (1-10 nM) of dopamine also potentiated D1 messenger RNA levels (up to 48%), resulting in a twofold increase in receptor levels. Transfection studies with the cloned human D1 promoter construct, pGL-D1P, indicated that the up-regulation of D1 messenger RNA was due to activation of promoter by dopamine. The dopamine-mediated up-regulation of both D1 receptor messenger RNA and promoter was prevented by the D1-selective antagonist, SCH 23390. The results suggest that dopamine regulates D1 receptor gene and protein expression in a bimodal manner, partly through activation of the receptor promoter. Moreover, the effects of dopamine are independent of the second messenger, cyclic AMP.
Disruption of the gene for Hsp30, an alpha-crystallin-related heat shock protein of Neurospora crassa, causes defects in import of proteins into mitochondria.
The gene for Hsp30, the only known alpha-crystallin-related heat shock protein of Neurospora crassa, was disrupted by repeat-induced point mutagenesis, leading to loss of cell survival at high temperature. Hsp30, which is not synthesized at 30 degrees C, associates reversibly with the mitochondria at high temperature (45 degrees C). In this study, we found that import of selected proteins into internal compartments of mitochondria, following their synthesis in the cytosol, was severely impaired at high temperature in a strain mutant in Hsp30. After 70 min of cell incubation at 45 degrees C, most matrix, inner membrane, and intermembrane-space proteins tested were reduced in import by about 50-70% in the mutant, as compared to wild-type cells. In contrast, assembly of selected proteins into the outer mitochondrial membrane was not reduced, except for one component of the preprotein translocase complex of the mitochondrial outer membrane. Three proteins of this complex co-immunoprecipitated with Hsp30 of wild-type cells incubated at 45 degrees C. We propose that Hsp30 interacts with the preprotein translocase of the mitochondrial outer membrane and that it chaperones the activity of one or more components of this translocase complex at high temperature.
HIV pioneer stands up for patients' rights.
Explore the source record for details and available documents.
Plane crash tests skills of local EMS providers.
Explore the source record for details and available documents.
UAMS unit charts course to paperless office.
Explore the source record for details and available documents.
Dr. Suzanne Klimberg. Professor juggles family, research, teaching.
Explore the source record for details and available documents.
Women gain ground with BreastCare. Program ensures screening, treatment for poor.
Explore the source record for details and available documents.
The doc stops here. Arkansas Medical Foundation helps doctors end drug abuse.
Explore the source record for details and available documents.
Collective bargaining is AMA's answer to managed care woes.
Explore the source record for details and available documents.
Emotional and behavioural difficulties in children with diabetes: a controlled comparison with siblings and peers.
This study describes a comparison of children with insulin dependent diabetes mellitus (IDDM) with two control groups: the sibling nearest to them in age, and peers matched for gender and age. A broad range of emotional and behavioural difficulties were assessed using the Conners' Teachers' and Parents' rating scales (CTRS 39 and CPRS 93). Individually completed children's questionnaires provided qualitative data about the children's problems. The results of the study showed markedly higher scores for the children with diabetes on the parent scales on factors such as hyperactivity, psychosomatic and restless/disorganized, in comparison to their siblings, but not their peers. These differences were not found on the Teachers' Scales. The findings are discussed in terms of parental perceptions of children with diabetes and the difficulties these children and their siblings experience in their lives. Suggestions are made for further research.
Regulation of Ca2+ transport by insulin and taurine. Interaction at the level of the Na(+)-Ca2+ exchanger.
Explore the source record for details and available documents.
Development and validation of a pig model for colon-specific drug delivery.
The purpose of this investigation was to develop a pig model for colonic drug delivery and to validate the model by determining whether the physiology of the pig colon had been significantly altered after the surgical implantation of a gut cannula into the terminal ileum of the pig. A fistula was created in the terminal ileum of the pig, and a cannula fitted for the purpose of directly administering drug formulations to a point just anterior to the ileocaeco-colonic valve of the gastrointestinal tract. The cephalic vein of the pig was also cannulated to enable continued blood sampling. Sulphasalazine was used as the model drug for the validation study. In the intact colon, sulphasalazine is metabolized by the gut microflora to sulphapyridine which is then absorbed. Sulphasalazine was administered orally to non-fistulated and fistulated pigs and then ileally, via the gut cannula, to fistulated pigs. Absorption of sulphapyridine was monitored by HPLC analysis of plasma samples. There was no significant difference in the absorption obtained for the three groups. Thus it is demonstrated that the colon physiology had not been altered. The colonic pig model is ideal for studying factors affecting the colonic absorption of drugs and as a means for developing drug delivery systems with improved absorption properties.
Architecture and health care: Saskatchewan leads the way in information technology.
Explore the source record for details and available documents.