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Biomedical subjects

N G Wright

Publications and source records attributed to N G Wright.

At least 55 records · Page 3Linked to original sources
PubMed1976

Canine leptospirosis: an immunopathological study of interstitial nephritis due to Leptospira canicola.

In an attempt to define the pathogenetic mechanisms involved in acute interstitial nephritis due to Leptospira canicola the kidneys of 14 dogs were subjected to histological immunofluorescence and elution studies. Leptospiral antigen was found in three sites within the kidney; as discrete organisms in the proximal tubules, as granular deposits in the cytoplasm of macrophages and in large extracellular clumps in the interstitium. Deposits of IgG were also detected in the latter site and in plasma cells found in the surrounding cellular infiltrate. Anti-leptospiral antibody, mainly of the IgG class, was detected in renal eluates using both agglutination-lysis and indirect immunofluorescence. From these findings it was considered that the cellular infiltrates found in the kidneys of dogs suffering from acute leptospiral nephritis have two main functions; firstly, the local production of anti-leptospiral antibody and, secondly, phagocytosis.

Animals↗
PubMed1976

An immunopathologic study of interstitial nephritis associated with experimental canine adenovirus infection.

The localisation of canine adenovirus in the renal tubules induces a marked cellular response in the interstitium. A number of mechanisms appear to be involved in the genesis of the lesions. Direct lytic damage to tubular epithelial cells by the virus is probably augmented by immunological factors. The production of local anti-viral antibody by plasma cells in the vicinity of virus-containing cells may result in the formation of toxic antigen-antibody complexes while the accumulation of large populations of macrophages suggests that cell-mediated hypersensitivity is also operative.

Adenoviridae Infections↗
PubMed1976

Experimental respiratory disease in dogs due to Bordetella bronchiseptica.

Young dogs of two age groups, six weeks and 12 weeks respectively, were infected by aerosol with a strain of Bordetella bronchiseptica which had been isolated from a dog with pneumonia. Clinical respiratory disease characterised by coughing and in some cases purulent nasal discharge was induced in both groups of infected dogs and also in dogs kept in contact. B bronchiseptica was recovered from the nasal cavity, trachea, bronchi and lung parenchyma of infected and contact animals. At necropsy, masses of Gram-negative bacteria were found trapped in the cilia of the respiratory epithelia and there was an exudate containing neutrophils in the mucosae of the respiratory tract at all levels. A close similarity was noted between the lesions produced in the dog and those described in pertussis infection in man. Experimental respiratory disease in the dog due to B bronchiseptica may offer a model system for the study of the human disease.

Animals↗
PubMed1976

Detection of immune complexes in the serum of dogs infected with canine adenovirus.

Evidence for the presence of circulating virus-immune complexes in dogs experimentally infected with canine adenovirus was found when deposits of canine IgG were present in the glomeruli of recipient mice. This passive transfer of complexes occurred only with serum taken at the height of the disease when antibody titres were low and when deposition of complexes in the donor dogs was maximal. Transfer of complexes was not obtained early in the disease (no circulating antibody and minimal deposition of complexes) nor later in the disease (high circulating antibody and minimal complex deposition).

Adenoviridae Infections↗
PubMed1975

Glomerular deposition of immune complexes in dogs following natural infection with canine adenovirus.

The renal lesions were studied in eight dogs which had either died as a result of acute canine adenovirus infection (Rubarth's disease) or were in various stages of recovery from the clinical disease. Using immunofluorescence techniques granular deposits of IgG were detected in the glomeruli of six dogs; four of these animals had similar glomerular deposits of canine adenovirus antigen. Eluates obtained from kidney tissue of four dogs were found to contain antiviral antibody. Histologically those animals in which glomerular deposits of IgG and viral antigen were detected showed segmental glomerular hypercellularity. These findings were attributed to the deposition of circulating virus antigen-antibody complexes in the glomeruli.

Adenoviruses, Canine↗
PubMed1975

Determination of the buoyant density of canine distemper virus by radioassay.

Canine distemper virus was labelled with tritiated uridine and, following precipitation with saturated ammonium sulphate solution, was concentrated 66-fold by centrifugation through a discontinuous sucrose gradient. When this preparation was centrifuged to equilibrium in density gradients of potassium tartrate or sucrose, radioactivity was distributed over the density range 1.218 to 1.180 with a pronounced peak at around 1.195. This corresponded closely to the distribution of infectivity and also to that of virus particles revealed by electron microscopy. In density gradients of caesium chloride, a plateau of radioactivity was present over the density range 1.26 to 1.24 with a peak at around 1.240 but most of the infectivity was limited to the range of 1.24 to 1.22. Since the amount of infectious virus recovered from potassium tartrate was greater than that recovered from the other two materials and the radioactive peak occurred over a narrower density range, it was concluded that potassium tartrate was the material of choice for the isopycnic centrifugation of canine distemper virus.

Ammonium Sulfate↗