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N G Picardo

Publications and source records attributed to N G Picardo.

9 recordsLinked to original sources
PubMed1981

Comparison of three immunological tests for seroepidemiological purposes in human echinococcosis.

The comparative sensitivity and specificity of three immunodiagnostic tests for hydatid disease--the latex agglutination (LA) test, the indirect haemagglutination (IHA) test and the counterimmunoelectrophoresis (CIEP) test--were studied. The results of the IHA test were evaluated using three positivity criteria. Antigen from the same source--hydatid cyst fluid pool of ovine origin--was utilized for the three tests. A total of 123 sera were studied; 30 preoperative sera from patients with other parasitic and infectious diseases, 30 sera from patients with non-parasitic diseases and 30 sera from normal blood donors. The results of the three tests were correlated to the immunoelectrophoresis (IEP) test based on the presence of arc 5. The same sensitivity (86.7%) and specificity (99%) was obtained in LA, CIEP and IHA tests when the minimum cross-reactivity titre criterion was employed for the latter. Non-specificity was relatively high (13%) in the IHA test when only serological reactivity was considered. The sensitivity of the IHA test decreased (83.3%) using the diagnostic titre criterion and non-specific reactions were eliminated. All three tests correlated well with the IEP test.

Antibodies↗
PubMed1980

[Correlative urinary excretion of sodium-uric acid in hyperuricemia (author's transl)].

Since uricosuria is an essential compensative factor in hyperuricemia, multiple factors exist which can influence on the uric acid excretion in the tubules. Normally, 90% of the uric acid filtered by the glomerulus is reabsorbed, and its active tubular secretion forms the greater part of the uric acid present in the urine. One of the factors observed as leading to urinary excretion of uric acid, is saline overload, although this effect cannot always be achieved. This study is an attempt to assess the importance of hyperuricemia as a determinant of the action of sodium upon the urinary excretion of uric acid. The series were grouped according to the plasma and urinary values of uric acid. In cases studied, the percentage of sodium was similar independent of the excretion percentages obtained for uric acid. Nevertheless, in the groups with increased plasma levels of uric acid, a direct and significant correlation was found between the excretion percentage of sodium and that of uric acid. These data cannot be obtained in groups with normal plasma levels of uric acid. Since saline overload does not always lead to hyperuricosuria, other factors must condition the secondary effect. The obtained results suggest that hyperuricemia can be one of those factors, offering a great amount of uric acid to the renal tubule, a condition in which the sodium may act as a dragging factor which may not be possible with a lesser degree of uric acid.

Adult↗
PubMed1979

[A new technical variation of the latex agglutination test for the diagnosis of human hydatidosis].

A new variant of the latex agglutination test using latex particles of 0,81 mu diameter is described. Hidatid cyst fluid antigen standardized by immunoelectrophoretic analysis was used. Fifty-one human sera from surgically confirmed hydatidosis and ninety control sera from non-hydatid diseases and healthy donors were studied. The method showed a sensibility of 86,2% and a global inespecificity of 1,1%. A human case of teniasis by Taenia saginata was positive. General considerations about the interpretation of results in human hydatid disease serology are made.

Echinococcosis↗