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Biomedical subjects

N G Gratz

Publications and source records attributed to N G Gratz.

14 recordsLinked to original sources

Education and employment of medical entomologists in Aedes aegypti control programmes.

Most dengue/DHF endemic countries have facilities for the training of entomologists. However, experience has shown that many of the graduates of such courses remain in the area of research and relatively few are employed in Aedes aegypti vector-control programmes. Few university courses in medical entomology provide specific training in vector control including such subjects as the organization and administration of vector control programmes and vector-control methods; chemical, biological and environmental control; evaluation of control activities and the epidemiology of the vector-borne diseases. Governments and municipalities are often unaware of the complexities of an efficient control programme and the importance of employing well trained entomologists to plan and implement them. Inadequately organized and poorly supervised vector-control programmes are often the reason for failure to interrupt transmission of the disease. The type of training required and the necessity for providing adequate career structures for vector control personnel and adequate funding for the control programmes is discussed.

Aedes

What role for insecticides in vector control programs?

Vector-borne diseases including dengue, yellow fever, Japanese encephalitis, malaria, leishmaniasis, and filariasis remain severe public health problems in most of the countries in which they are endemic. In some cases, their incidence is increasing and they are spreading to new geographic areas. For a number of the infections, the most effective manner of controlling their transmission is through control of their vectors. However, in some instances, such as dengue and Chagas' disease, there is no alternative. Most countries that are endemic for vector-borne diseases maintain vector control services, and most large tropical and semitropical cities also have pest control programs, mainly against pest mosquitoes. Virtually all of the vector and pest control programs depend on the use of insecticides formulated as larvicides, adulticides, baits, or insecticide impregnated bed nets. For many years, the development of new insecticides for use in public health programs was encouraged and supported by multilateral and bilateral health agencies, including the implementation of field trials in endemic areas. Due to the development of insecticide resistance, toxicologic and environmental considerations, and the cost of development and of registration, the number of compounds available for use has declined while the number of new insecticides submitted for laboratory and field trials to the World Health Organization has dwindled even more. The recrudescence of vector-borne diseases, the rapid pace of urbanization, lagging development of environmental services in many tropical cities, and difficulties encountered in ensuring the community's cooperation in its own protection through environmental measures make imperative the continued availability of pesticides for public health use. Since only the pesticide manufacturing industry has the combination of technical and financial resources to promulgate the research and development of new pesticides and pesticide groups, it is suggested that governments, bilateral, and multilateral organizations explore the manner in which they can assist industry in the development of new compounds and guarantee the continued availability of effective and safe pesticides for vector-control programs.

Animals

Lessons of Aedes aegypti control in Thailand.

The incidence of dengue haemorrhagic fever (DHF) in Thailand has increased cyclically since the first recognized outbreak in 1958. Without an effective vaccine against dengue, and considering the clinical difficulty of treating DHF cases, vector control is needed to prevent dengue transmission. Since the establishment of the WHO Aedes Research Unit in 1964, continued since 1973 as the WHO Collaborating Centre at the Department of Medical Research in Bangkok, much operational research has been carried out in Thailand on the bionomics and control of dengue vectors: Aedes aegypti and Ae. albopictus. This review shows that, as in most other countries, dengue vector control programmes in Thailand make little use of the procedures arising from research, nor have they reduced the upward trend of dengue or prevented DHF outbreaks. Implications of the reluctance to use results of operational research on vector control are considered and remedial suggestions made.

Aedes

Emergency control of Aedes aegypti as a disease vector in urban areas.

Techniques for the emergency control of adult Aedes aegypti populations and their development are reviewed. Larviciding and environmental measures provide only delayed control of adult populations. Large-scale field trials of the ultra-low volume application of insecticide concentrates in Southeast Asia, South America and Africa, using aerial, ground, vehicle-mounted and hand-carried equipment, have, in most cases, resulted in satisfactory levels of control of adult populations. Sequential or indoor ULV applications of fenitrothion have provided immediate control and sustained reduction of the adult populations, often lasting well through normal peak transmission periods of dengue. Many ULV application trials in the Caribbean have not produced satisfactory control, but it is considered that this was due to the type of house construction, to the lower dosage rates of the malathion 96% ULV concentrates used, or to inappropriate droplet sizes. While ULV applications can provide rapid and effective emergency control of vectors at the time of outbreaks of disease in urban and periurban areas, they should not be used as a routine mosquito control measure nor as an alternative to reducing vector populations by environmental measures.

Aedes

Problems associated with the control of rodents in tropical Africa.

As elsewhere in the world, rodents are responsible for very considerable economic losses in tropical Africa because of their depredations on both growing crops and stored food products. Unfortunately, few accurate data are available on the extent of these losses but there is evidence that they are considerable. The public health importance of rodents, both as reservoirs and vectors of disease in tropical Africa, is also great; plague, leptospirosis, murine typhus, and Lassa fever are among the diseases associated with rodent hosts. Scientifically based rodent control programmes have been carried out in very few areas of Africa and there is urgent need for studies and demonstrations on rodent control in both urban and rural areas. The problems likely to be encountered are reviewed and methods of control proposed.

Africa

The structure of rodent faunas associated with arenaviral infections.

The biogeographical examination of rodent faunas associated with arenaviruses reveals two distinct patterns. Lymphocytic choriomeningitis (LCM) virus is associated primarily with a single murid species, Mus musculus, although it is also known to cause laboratory infections in other species. On the other hand, the arenaviruses from the Western hemisphere are associated exclusively with a large and diverse group of cricetid rodents. Studies to date, although limited, have not demonstrated their association with any other rodent groups, although in South America alone at least twelve other rodent families are known. Evidence at the present time indicates that Lassa virus is only associated with a common African rodent, Mastomys natalensis. From this limited evidence it is as yet difficult to determine whether Lassa virus will follow the pattern of the South American arenaviruses, most of which are known from several species of rodents, or that of LCM virus, which appears to be associated with only a single rodent species. In this paper, the history and structure of South American, Eurasian, and African rodent faunas are described.

Africa

Determination of the age of some anopheline mosquitos by daily growth layers of skeletal apodemes.

Earlier work has shown that it is possible to determine the calendar age of Culex and Aedes mosquitos by counting the daily layers of cuticular growth on the inner apodemes. In view of the epidemiological importance of age grouping in Anopheles species, the applicability of the method to this genus was studied in both laboratory-reared and field-collected specimens. In the four species examined, it was found possible to distinguish daily growth layers for periods of up to 10-13 days provided that proper staining procedures are used.

Age Determination by Skeleton

A critical review of currently used single-dose rodenticides.

The introduction of the anticoagulants in the early 1950s, with their much greater safety to nontarget animals, resulted in a general decline in the use of single-dose rodenticides. However, the appearance of rodent resistance to the anticoagulants, first in the United Kingdom, later elsewhere in Europe, and still more recently in the USA, has revived interest in the use of single-dose rodenticides. Unfortunately, owing to their danger to nontarget mammals, the use of several of these compounds must be restricted; others, despite their long use, are now recognized to be unsatisfactory because of their poor acceptance or reacceptance by rats and mice. Thus, only very few compounds of this type are available for unrestricted use and there is an urgent need for the development of effective alternatives.

Amides

Age determination of some flies and mosquitos by daily growth layers of skeletal apodemes.

A new method of determining age in certain Diptera, including Culex and Aedes mosquitos, involves counting-sometimes with the aid of staining techniques-the layers of cuticle growth that form daily at different points (inner apodemes) of the skeleton. If developed, the method would be of great importance for eradication and control programmes. The authors recommend further studies, in particular on the application of the technique to Anopheles and certain flies such as Calliphora.

Age Determination by Skeleton

Preliminary studies on the use of ultra-low-volume applications of malathion for control of Aedes simpsoni.

In view of the threat of additional yellow fever epidemics in East Africa and recent successes in the use of malathion applied by the ultra-low-volume technique against insect vectors, field trials were initiated in November 1968 to test the efficacy of this method against Aedes simpsoni. Results of these trials show that in areas where Musa ensetta is the principal breeding site, the application of 20.2 US fl oz/acre (1474 ml/ha) of malathion was capable of reducing vector populations 93%-100%. Comparable applications of 6 US fl oz/acre (438 ml/ha) reduced populations by 76% and 89%. Since this study did not conclusively demonstrate that the 6 US fl oz/acre dosage rate was sufficient to obtain maximum population reduction, further studies should be initiated to obtain information on the optimal dosage to be employed against this species.Under the conditions of these trials, the use of a light, single-engined aircraft equipped with a rotary atomizer spray system and capable of operation from improvised fields was shown to be feasible against this vector species. It was further demonstrated that the canopy formed by M. ensetta does not present an impossible barrier to the penetration of the spray droplets produced by the ULV application technique.Observations made on the application methodology and on the biology of A. simpsoni show the versatility of this species and the need for further studies on the integration of spray applications with the peak activity periods of the insect.

Aedes

Ortho-isopropoxphenyl methylcarbamate (OMS-33) as a residual spray for control of anopheline mosquitos. With special reference to its evaluation in the WHO programme for evaluating and testing new insecticides.

More than 1300 compounds have so far been included in the WHO Programme for Evaluating and Testing New Insecticides, which is designed to disclose compounds that may satisfactorily replace those to which insect vectors of disease have become resistant. The authors describe the successful passage of o-isopropoxyphenyl methylcarbamate (OMS-33) through the first 6 stages of the 7-stage programme that has been established for compounds intended for use against anopheline mosquitos and conclude that this product is suitable for testing in the final stage-large-scale epidemiological evaluation.In operational field trials (at 2 g/m(2)) OMS-33 has been shown capable of controlling Anopheles stephensi (in Iran), An. gambiae and An. funestus (in Nigeria) for 3-4 months, An. albimanus (in El Salvador) for 2-4 months and An. dthali (in Iran) for 2(1/2) months. It has an airborne effect by which anophelines are killed for a considerable time after OMS-33 has been sprayed, even though they do not make contact with a sprayed surface; this quality would appear advantageous in areas where anophelines enter houses and bite man but do not rest long enough on sprayed surfaces to acquire a lethal dose of insecticide or where significant outdoor biting occurs. The observance of simple safety precautions protects occupants of sprayed houses, spraymen and others from danger. Chemical studies have indicated that commercially produced water-dispersible powders of OMS-33 are stable under field conditions of storage and use.

Aerosols