Search PubMed⌕ Search

Biomedical subjects

N Fujii

Publications and source records attributed to N Fujii.

At least 523 records · Page 29Linked to original sources
PubMed1985

Studies on peptides. CXXVIII. Application of new heterobifunctional crosslinking reagents for the preparation of neurokinin (A and B)-BSA (bovine serum albumin) conjugates.

A decapeptide corresponding to the entire amino acid sequence of neurokinin A, a porcine spinal cord peptide, was synthesized in a conventional manner using protecting groups removable by 1 M TFMSA-thioanisole in TFA. The HS-CH2CH2CO group was introduced onto the synthetic neurokinin A by reaction of 3-(S-acetyl-thiopropionyl)-thiazolidine-2-thione, followed by deacetylation with hydroxylamine. 2,4-Dinitrophenyl-p-(beta-nitrovinyl)-benzoate trapped the above HS-CH2CH2CO-neurokinin A derivative in acidic media, then BSA in basic media in nearly quantitative yield. A similar decapeptide, neurokinin B, was also synthesized and conjugated onto BSA using an alternative SH-introducing reagent, 3-(S-p-methoxybenzyl-thiopropionyl)-thiazolidine-2-thione, and the above heterobifunctional conjugating reagent.

Amino Acid Sequence↗
PubMed1984

Oligo-2',5'-adenylate synthetase activity in peripheral blood mononuclear leukocytes in various diseases.

Interferon induces oligo-2',5'-adenylate synthetase in cells. In various diseases, interferon was detectable in the circulation or was produced spontaneously from peripheral blood mononuclear leukocytes. The oligo-2',5'-adenylate synthetase activity in peripheral blood mononuclear leukocytes was examined in various diseases, including systemic lupus erythematosus, sarcoidosis, Vogt-Koyanagi-Harada disease, and Behcet's disease. The activity of this enzyme was significantly increased in systemic lupus erythematosus (P less than 0.01), sarcoidosis (P less than 0.01), and Vogt-Koyanagi-Harada disease (P less than 0.01) compared with that in controls, but the increase of activity was not significant in Behcet's disease (P greater than 0.05).

2',5'-Oligoadenylate Synthetase↗
PubMed1984

Studies on peptides. CXIII. Synthesis of the heptacosapeptide amide corresponding to the entire amino acid sequence of chicken secretin.

The heptacosapeptide amide corresponding to the entire amino acid sequence of chicken secretin was synthesized by assembling four peptide fragments followed by deprotection with thioanisole-mediated trifluoromethanesulfonic acid in TFA. The deprotected peptide was purified by gel-filtration on Sephadex G-25, followed by ion-exchange chromatographies on CM-cellulose and DEAE-Sepharose. Synthetic peptide stimulated the flow of pancreatic alkaline juice and decreased systemic blood pressure in rats.

Amino Acid Sequence↗
PubMed1983

Subsets of lymphoid cells in blood and thymus in myasthenia gravis. Monoclonal antibody analysis.

Lymphoid cell subpopulations in peripheral blood and thymus were analyzed in patients with myasthenia gravis (MG) using monoclonal antibodies. The proportion of lymphocytes of T lineage (OKT 3+, OKT 4+, OKT 8+ cells) in peripheral blood of 11 MG patients, was significantly decreased in comparison with controls, but the ratio of OKT 4+/OKT 8+ cells was not different. Thymus cells were studied in 9 patients. The percentage of OKIa 1+ cells was significantly higher in MG thymus than in control thymus (P less than 0.0005). There were no significant differences in the proportions of T lymphocyte subsets between MG and control thymuses.

Adult↗
PubMed1983

Classification of malar complex fractures using computed tomography.

Fifty-one patients with malar complex fractures were examined with computed tomography to analyze the pattern of displacement in an anteroposterior direction of the face. Malar complex fractures were classified into four groups according to the location and direction of the fragments. Comparisons of the fracture classification obtained by use of CT images with that obtained by conventional radiographic methods demonstrated sufficient correlations between the classification systems to confirm that it is possible to establish a complete three-dimensional pattern of displacement in malar complex fractures using CT imaging.

Humans↗