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Biomedical subjects

N Frank

Publications and source records attributed to N Frank.

At least 91 records · Page 5Linked to original sources
PubMed1982

[Enhancement of the toxic effect of endoxan after pretreatment with brain homogenates in rats].

Following monotherapy with cyclophosphamide rat leukemia cells (L 5222) survive only in the CNS, as has been described previously. Here we report on investigations performed to elucidate the influence of rat brain tissue on cyclophosphamide activity. It was found that the toxicity of cyclophosphamide is enhanced 5 fold after incubation with freshly prepared brain homogenate. In parallel experiments it was also found that cyclophosphamide preincubated with brain homogenates is therapeutically less effective toward L 5222 cells than cyclophosphamide alone. The incubation of cyclophosphamide with liver homogenate did not result in the same enhancement of cyclophosphamide toxicity nor in the decrease of therapeutic effects. The different influence of the two tissues on cyclophosphamide suggests two different metabolic mechanisms.

Animals↗
PubMed1981

[Diagnosis of space-occupying renal processes: sonography and aimed fine-needle biopsy].

It is reported on 3 cases in which by sonographic examination a tumorous process could be established. In all cases the additional investigation by fine needle biopsy and cytological examination proved the presence of malignant cells. This local renal process proved by sonography could be verified neither by i.v. pyelographic nor arteriographic methods. For two patients the diagnosis based on sonography, fine needle puncture and cytological test could be verified by operation. The third patient was not operated in spite of malignant cells in the cytogram because of negative computer tomography provided by an external hospital.

Aged↗
PubMed1981

[The normal pancreas. Demonstration in the sonogram in relation to age].

For 191 patients without any clinically or anamnestically traceable disease of pancreas sonographic inspection was performed, a real time-equipment type Aloka, SSD 240 was used. The age of the patients, divided into 5 groups ranged from 15 to 90 years. The measurements of the pancreas sonographic inspection was performed, a real time-equipment type Aloka, SSD 240 was used. The age of the patients, divided into 5 groups ranged from 15 to 90 years. The measurements of the pancreas were established for head, neck, corpus and tail respectively. The following mean values for the thickness were found: Head: 2,41 +/- 0,41 cm; neck: 1,53 +/- 0,34 cm; corpus: 1,90 +/- 0,37 cm; tail: 1,67 +/- 0,37 cm. The width of the organ was in the area of the head: 1,3 to 3,5 cm, neck 1,0 to 2,7 cm, corpus 1,1 to 3,0 cm, tail 0,8 to 2,6 cm. A comparison between normal weight- and overweight-patients showed no significant differences at all. The echostructure of the pancreas showed a clear increase of intensity with progress in age. The reason for this more detailed echostructure is seen in an infiltration of fat as well as collagenous and fibrous elements. No change in organ size was found in this connection.

Adult↗
PubMed1981

[Duodenal ulcer in the aged. Diagnosis, therapy and disease course].

In 68 of 1152 patients older than 65 years an acute ulcus duodeni could be stated by endoscopy. This is 5,9% of the examined patients. Among this group there was a remarkably great number of patients who belonged to the age group between 70 and 80 years. The anamnesis of 42 patients indicated complaints for several years, whereas 26 patients showed symptoms only for a few weeks. The size of the acute ulceration was mostly below 1,0 cm. A determined relation between the size of the ulceration, the patients age and the duration of their anamnesis could not be found. In 12 patients an acute ulcus ventriculi could be proved simultaneously. In 15 cases acute bleeding was the initial symptom of the ulceration. Healing of the ulcera took between 4 weeks and 3 months. The conservative therapeutical steps were not standardized. Stationary and ambulant patients did not show any difference in the time of healing. It was remarkable that the consumption of alcohol and especially of cigarettes was relatively high.

Acute Disease↗
PubMed1980

Effect of disulfiram on the alkylation of rat liver DNA by nitrosodiethylamine.

The extent of ethylation of guanine in 7 N and O 6 position in rat liver DNA was studied after chronic feeding with unlabelled nitrosodiethylamine followed by a single application of the 14C labelled drug. Disulfiram inhibits the alkylation of rat liver DNA. It was also shown that a discontinuous dosage of disulfiram protects against the alkylating effect of 14C nitrosodiethylamine only for a distinct period of time.

Alkylation↗
PubMed1977

Inhibition of diethylnitrosamine-induced strand breaks in liver DNA by disulfiram.

Disulfiram (DSF) delayed the appearance of diethylnitrosamine (DEN)-induced strand breaks in liver DNA of rats. The fragmentation of liver DNA produced by DEN was studied 4 and 24 h after administration of the carcinogen on alkaline sucrose gradients. A single dose of 500 mg/kg DSF given 1 h prior to carcinogen treatment delayed for at least 4 h the DEN--induced strand breaks in liver DNA. A single DSF pretreatment, however, did not protect against carcinogen-induced strand breaks when observed 24 h after DEN injection. It is possible that continuous administration of DSF might inhibit the DEN-produced damage of the genetic material.

Animals↗
PubMed1977

Klebsiella serotype-13 capsular polysaccharide: primary structure and depolymerization by a bacteriophage-borne glycanase.

Periodate oxidation and Smith degradation, methylation analysis including uronic acid degradation, partial hydrolysis with acid, bacteriophage degradation, and p.m.r. spectroscopy have been used to elucidate the primary structure of the Klebsiella serotype-13 capsular polysaccharide. The polymer consists of pentasaccharide repeating-units comprising a 4)-beta-D-Manp-(1 leads to 4)-alpha-D-Glcp-(1 leads to 3)-beta-D-Glcp-(1 leads to chain with a 3,4-O-(1-carboxyethylidene)-beta-D-Galp-(1 leads to 4)-alpha-D-GlcAp-(1 leads to branch at position 3 of the mannose. It is shown that there is a glycanase activity associated with particles of Klebsiella bacteriophage No. 13, which catalyses hydrolysis of chain beta-D-Glcp-(1 leads to 4)-beta-D-Manp linkages in the type-13 polysaccharide. The chemical basis of some serological cross-reactions of the Klebsiella K13 antigen is discussed.

Antigens, Bacterial↗
PubMed1975

The structure of Klebsiella serotype II capsular polysaccharide.

Using periodate oxidation, methylation analysis, the characterization of oligosaccharides obtained by partial acid hydrolysis, p.m.r. spectroscopy, and analytical ultracentrifugation, the structure of the (mildly alkali-treated) Klebsiella serotype 11 capusular polysaccharide has been elucidated. The tetrasaccharide repeating-unit comprises the sequence yields 3)-beta-D-Glcp-(1 yields 3)-beta-D-GlcUAp-(1 yields 3)-alpha-D-Galp-(1 yields with a 4,6-O-(1-carboxyethylidene)-alpha-D-galactosyl residue linked to O-4 of the glucuronic acid residue. The structural basis for some serological cross-reactions of the Klebseilla K11 antigen is discussed, and it is shown that rabbit antisera against the Klebsiella K11 test-strain predominantly contain K agglutinins specific for branch-terminal 4,6-O-(1-carboxyethylidene)-D-galactose.

Animals↗
PubMed1975

Escherichia coli capsule bacteriophages. IV. Primary structure of the bacteriophage 29 receptor, the E. coli serotype 29 capsular polysaccharide.

Using periodate oxidation, methylation analysis, characterization of oligosaccharides by Smith degradation or partial acid hydrolysis, as well as proton magnetic resonance, the primary structure of the Escherichia coli serotype 29 capsular polysaccharide (the receptor of E. coli K phage 29) was reinvestigated. The polymer was found to consist of hexasaccharide repeating units of the following structure: (see article).

Binding Sites↗