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Biomedical subjects

N E Kay

Publications and source records attributed to N E Kay.

135 records · Page 8Linked to original sources
PubMed1977

Monocyte-erythrocyte interaction in vitro in immune hemolytic anemias .

Monocyte recognition of immunoprotein-coated erythrocytes in vitro was studied in 21 patients with immune hemolytic anemia. Simultaneous assessment of immunoglobulin and complement components on the patients' erythrocytes was performed. The patients were heterogeneous in relation to associated diseases, therapy, and duration of disease state; 15 were studied within 1 mo of diagnosis and 4 additional patients were studied in sequential fashion. Monocyte-erythrocyte interaction was assessed by both a morphologic and a radioactive assay; 12 of 17 patients had elevated ratios in the morphologic assay and 15 of 17 patients had elevated ratios in the radioactive assay. The majority of patients exhibited enhanced interaction between their monocytes and autologous immunoprotein-coated erythrocytes, as compared to normal moncytes exposed to the same immunoprotein-coated erythrocytes. These findings suggest that there is an enhanced capacity of monocytes from certain patients with immune hemolysis to interact with autologous immunoprotein-coated erythrocytes in vitro.

Anemia, Hemolytic, Autoimmune↗
PubMed1975

Abnormal erythrocyte metabolism in hepatic disease.

Erythrocyte (RBC) metabolic studies were done on 114 patients with severe hepatic disease. Heinz body formation after incubation of RBCs with acetyl phenylhydrazine was found to be significantly higher in patients than in controls. RBC-reduced glutathione levels were lower than those of controls both before and after incubation with acetyl phenylhydrazine, and patients with the highest Heinz body counts had the lowest reduced glutathione levels. RBC methylene blue-stimulated hexose monophosphate (HMP) shunt metabolism and glucose recycling through the shunt were significantly lower in patients with active hepatic disease than in controls. There was no difference in resting HMP shunt activity or in resting recycling of glucose. Despite impairment of shunt metabolism, total glucose consumption was greater in patients than in controls. The patients with the lowest stimulated HMP shunt metabolism and glucose recycling had the highest Heinz body counts, lowest reduced glutathione, and highest total glucose consumption. A continuum of abnormal shunt metabolism was seen, from a mild reduction of stimulated HMP shunt activity to a severe combined decrease in both the HMP shunt and glucose recycling. When measured, glutathione reductase, glutathione peroxidase, glucose-6-phosphate dehydrogenase, and transketolase were normal or increased. Sequential studies were done on 11 patients who had abnormal metabolic studies. Coincident with improvement of HMP shunt metabolism, the Heinz body counts became lower, reduced glutathione higher, hematocrit higher, and liver function improved. Impaired HMP shunt metabolism appears to be a common, acquired RBC abnormality in patients with severe, active liver disease.

Erythrocytes↗