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Biomedical subjects

N Dusitsin

Publications and source records attributed to N Dusitsin.

At least 37 records · Page 2Linked to original sources
PubMed1982

A rapid method for the determination of 5 alpha-dihydrotestosterone in Thai males receiving medroxyprogesterone acetate.

A rapid method for the determination of DHT (5 alpha-dihydrotestosterone) in serum was established, utilizing potassium permanganate as an oxidising agent to convert the double bond at position 4-5 of testosterone to dihydroxy alcohols. DHT was then extracted with diethyl ether and measured directly using radioimmunoassay technique. The precision of the assay was determined at three levels of DHT added into pooled serum and found to be 4.4-5.1% for between assay percentage coefficient of variation. The sensitivity of the standard curve was found to be 4.2 pg/0.5 ml sample. The accuracy was determined in pooled serum with added standard DHT in the range of 80-800 pg. The percentage recovery of the assay was found to be 92.93-98.35%. The antisera used for DHT assay exhibited no significant cross-reaction with various steroids tested except testosterone which showed a 91.3% cross-reaction. The method was applied to measure the level of DHT in serum of Thai males receiving medroxyprogesterone acetate (MPA) as an oral contraceptive. Twenty-five subjects were divided into 5 groups. Group 1 received an oral placebo with monthly sesame oil injection. Groups 2, 3 and 4 received 5 mg, 10 mg and 20 mg MPA, respectively, with the addition of monthly injection of 250 mg testosterone enanthate (TE). Group 5 received 20 mg MPA daily with the addition of monthly injection of 500 mg TE. Blood specimens were obtained monthly. The first 4 months were used as a control period. After the 4-month period, each subject received treatment as mentioned above for 6 months, then follow-up study was resumed for another 6 months. The results revealed that subjects receiving 20 mg MPA with the addition of 250 mg TE showed a significant decrease of DHT levels (p less than 0.001).

Contraceptive Agents, Male↗
PubMed1981

Hormonal changes in normal menstrual cycle of Thai women.

The concentrations of luteinizing hormone (LH), follicle stimulating hormone (FSH), prolactin (PRL), progesterone (P), estradiol (E2), testosterone (T) and cortisol have been measured by radioimmunoassay technique in daily serum samples of 10 Thai women throughout 10 menstrual cycles. The results show that in 10 subjects studied, 9 subjects show the patterns and levels of LH, FSH, E2 and progesterone suggestive of having ovulatory cycles. One subject shows no patterns of these hormones indicative of having ovulatory cycle. Daily serum PRL, T and cortisol concentrations, when determined for each individual subject for the entire cycle, varied among the 9 subjects studied. During the menstrual cycle, changes of hormones were observed in the women studied. There was a significant rise in the concentrations of serum PRL (2 subjects), T (2 subjects) and cortisol (3 subjects) on the day of the LH surge. When the mean levels of PRL, T and cortisol were plotted for 9 subjects in relation to the LH peak, there was no highly significant rise in the concentration of serum PRL, T and cortisol at midcycle.

Estradiol↗
PubMed1980

Post-partum tubal ligation by nurse-midwives and doctors in Thailand.

A shortage of doctors limits the provision of post-partum sterilisation services in rural areas of Thailand. To overcome this problem nurse-midwives with theatre experience were trained to perform post-partum tubal ligation by a mini-laparotomy incision under local anaesthesia. The performance of the nurse-midwives was compared with that of doctors in a controlled, randomised clinical trial. Some operative difficulty was encountered by the nurses in 4.9% of cases and by the doctors in 2.0% of cases. This difference is not statistically significant and arose largely because the nurse-midwife cases were more obese. Nurse-midwives required a significantly longer operating-time (18.5 min) than doctors (11.9 min). However, postoperative morbidity was similar in the two groups (7.0% and 6.0%, respectively). These results suggest that trained nurse-midwives with theatre experience can safely provide post-partum sterilisation services. A further field trial is underway.

Clinical Trials as Topic↗
PubMed1980

The effect of injectable norethisterone oenanthate on ovarian hormones in Thai women.

The ovarian hormones, progesterone and estradiol, as well as norethisterone concentrations were studied in 15 subjects receiving a single intramuscular injection of norethisterone oenanthate. Three treatment groups were studied. The 5 subjects of group A had no additional treatment, the 5 subjects of group B received ethynylestradiol and the 5 subjects of group C received combined ethynylestradiol and norgestrel orally. Additional treatment was for 5 consecutive days 6 weeks after the injection. Blood samples were obtained weekly for 12 weeks post-treatment. All subjects showed a similar pattern of norethisterone concentrations with a range of 3.5-19.5 ng/ml 1 week after injection, declining to levels of less than 0.02 ng/ml at the end of treatment period. Progesterone concentrations of greater than 4 ng/ml indicated that ovulation had occurred in 2 subjects of group A, 5 subjects of group B and 2 subjects of group C during the study period. Serum estradiol concentrations reached pre-ovulatory levels (greater than 200 pg/ml) in 4,3 and 2 subjects of group A,B and C, respectively, during the treatment period. With the other subjects, serum estradiol showed lesser increases. Among the 15 Thai women in the study, the median time of ovulation was 11 weeks, with the earliest occurring at 6 weeks. Three women receiving NET-OEN and no other treatment had ovulated by 6 weeks. Thai women in this study appear to be more resistant to the antiovulatory effect of NET-OEN as compared to women in other published studies.

Adult↗
PubMed1977

The release of prolactin by medroxy-progesterone acetate in human subjects.

Medroxyprogesterone acetate an injectable contraceptive when administered to four lactating women at a dose of 150 mg every three months significantly raised prolactin levels over those observed in four control women. Prolactin levels were higher at all times during 4-17 weeks in the drug-treated women, irrespective of whether the samples were collected before or after suckling. It was also observed that while suckling clearly released prolactin in both groups the difference was greater in the women receiving the injection, indicating that the drug enhanced the release of prolactin in response to the suckling stimulus.

Adult↗
PubMed1976

The effect of D-norgestrel, 30 micrograms, on the oral glucose tolerance test, including insulin levels, in Thai women.

The effect of D-norgestrel, 30 mug, on the oral glucose tolerance test was studied in 49 Thai women. There was a significant elevation of the blood glucose level at 60 minutes during the test in women who had taken D-norgestrel for 6 and 12 months. Insulin levels in the blood were significantly elevated over control levels in both groups of women at 90, 120, 150, and 180 minutes during the test. There was no difference in the results obtained at 6 months and 12 months. There was also no significant difference in the fasting blood glucose or insulin levels in the three groups of women. The results indicate that D-norestrel at a daily dose of 30 mug has an effect on carbohydrate metabolism in Thai women.

Administration, Oral↗
PubMed1976

A study of the use of intermittent serum luteinizing hormone, progesterone and oestradiol measurements for the detection of ovulation.

The value of luteinizing hormone (LH), progesterone (P) and oestradiol (E2) in serum as an index of ovulation and corpus luteum function was studied in blood samples collected at infrequent intervals during the menstrual cycle from 19 healthy Thai women. A serum P level of more than 3 ng/ml was detectable in 70 to 80 per cent samples obtained between days 20 and 25 and a P peak and/or E2 peak (greater than 150 pg/ml) were found at this time in 85 to 94 per cent of the cycles. In not more than 26 per cent of the cycles was an LH peak (greater than 150 ng/ml-LER-907) detected when all results were considered. A significant P and/or E2 peak on day 22 or 23 occurred in more than 78 per cent of 25 to 33 day cycles, whereas, the P and/or E2 peak was detectable in only 50 per cent of the cycles lasting less than 25 or more than 33 days. The postovulatory serum P levels were the ones most consistently raised in patients studied over several consecutive cycles.

Adolescent↗
PubMedDate not supplied

Age at menarche in Thai girls.

Age at menarche and menstrual symptoms were recorded in a sample of Thai schoolgirls in Bangkok. Using a random sampling proportional to size from this target population, structured questionnaires were offered to a sample of 15998 girls of grades 4-12. Fifty-six per cent of the study population was menarcheal during the time of study. Average mean ages by recalling age at menarche and by probit analysis were 12.51 +/- 1.17 and 12.35 +/- 1.41 years, respectively. The onset of menstruation occurred most frequently in April and October. Abdominal pain was the most common symptom during the menstrual period. Acne, malaise and back pain were other common complaints.

Abdominal Pain↗