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N Desplaces

Publications and source records attributed to N Desplaces.

At least 37 records · Page 2Linked to original sources
PubMed1992

[BCG osteoarthritis on knee prosthesis after intra-vesical BCG therapy].

A case of Calmette-Guérin bacillus (BCG) infection of a knee implant during intravesical BCG-therapy is reported. The course was favorable after replacement of the implant and administration of antituberculous agents. This case of septic osteoarthritis due to the BCG is different from cases of reactive polyarthritis reported after intravesical instillations of BCG. It probably resulted from diffusion of the BCG via the bloodstream.

Administration, Intravesical↗
PubMed1988

Monoclonal antibody reactivity as a virulence marker for Legionella pneumophila serogroup 1 strains.

Using a panel of nine monoclonal antibodies, we subgrouped 85 environmental and 129 clinical Legionella pneumophila serogroup 1 isolates from Paris, France. Patients were unlikely to be epidemiologically linked either with each other or with the 44 sampled environmental sites (14 air conditioning systems and 30 buildings) that were selected at random in the Paris area. According to their monoclonal antibody patterns, isolates belonged to 14 subgroups. Monoclonal antibody 2 recognized 121 (93.8%) of 129 clinical isolates and 30 (35.3%) of 85 environmental isolates (P less than 10(-9)). Of the eight patients infected with L. pneumophila not recognized with monoclonal antibody 2, seven were immunocompromised; only 46.3% of the 121 patients infected with L. pneumophila reactive with monoclonal antibody 2 were immunocompromised (P = .02). We conclude that monoclonal antibody 2 can be used as a marker for the more virulent strains of L. pneumophila serogroup 1.

Antibodies, Monoclonal↗
PubMed1988

Urease-positive thermophilic Campylobacter (Campylobacter laridis variant) isolated from an appendix and from human feces.

Urease-positive thermophilic campylobacters were isolated for the first time from the feces of two adults with diarrheal disease and from the appendix of a child with appendicitis. They were identified as Campylobacter laridis by a hybridization dot blot assay. Urease testing should be included in the tests used for the identification of campylobacters at the species level, even for those strains which are not of gastric origin.

Appendix↗
PubMed1987

[Chlamydia trachomatis perityphlitis, an anatomo-clinical entity? Study of 3 cases].

The authors describe three cases of perityphlitis secondary to Chlamydia trachomatis infection and revealed at laparotomy. Clinically, the symptoms invariably mimicked acute appendicitis. The pathogen was identified by a direct immunofluorescent assay with specific monoclonal antibodies (Microtrak). Prompt cure was achieved by doxycycline therapy. Thus, peroperatively, the discovery of an isolated perityphlitis with a normal appendix should raise the possibility of a C. trachomatis infection, even if there is no salpingitis.

Adult↗
PubMed1986

The new quinolones and their combinations with other agents for therapy of severe infections.

New quinolones are very potent compounds against Gram-negative bacteria, including Pseudomonas, and staphylococci. They are particularly interesting because of their oral availability and their pharmacokinetic properties. When combined with other common antibiotics they appear to have mostly indifferent effects in vitro. Clinical results have shown their efficacy when used alone for the treatment of osteomyelitis, complicated urinary tract infections and prostatitis. Nevertheless, in 6% of cases resistant strains were selected (Klebsiella, Enterobacter, Serratia, P. aeruginosa and staphylococci). The use of combinations of quinolones with other antibiotics was successful in the treatment of osteomyelitis and severe nosocomial infections including bacteraemia, and seems to prevent emergence of resistant strains.

Anti-Bacterial Agents↗
PubMed1985

DNA probe specific for Legionella pneumophila.

A procedure for preparing a DNA probe to be used in the specific detection of Legionella pneumophila by dot or colony hybridization has been devised. When total DNA from L. pneumophila was used as a radioactive probe, cross-hybridization occurred with DNA from many other species belonging to various families (including Legionellaceae, Enterobacteriaceae, Pseudomonadaceae, and Vibrionaceae). Cross-hybridizing restriction fragments in L. pneumophila ATCC 33152 DNA were identified on Southern blots. When unlabeled DNA from strain ATCC 33152 was cleaved by endonuclease BamHI, the DNA fragments cross-hybridizing with the labeled DNA from all of the other species and genera tested (or with Escherichia coli 16 + 23 S RNA) had a size of 21.4 and 16.2 kilobase pairs (major bands) and 28.0, 12.8, and 10.1 kilobase pairs (minor bands). BamHI restriction fragments of L. pneumophila DNA deprived of the cross-hybridizing fragments were pooled and used as a probe for the detection of L. pneumophila. This probe proved to be specific for L. pneumophila in colony and dot hybridization. It can potentially be used for the detection of L. pneumophila in clinical and water samples. The procedure described can be readily applied to the preparation of probes specific for phylogenetically isolated bacterial species other than L. pneumophila.

Base Sequence↗
PubMed1984

[Survey of Legionella in the Parisian environment. Practical implications].

A qualitative and quantitative survey of environmental Legionella in the Paris area was undertaken because of its epidemiological implications. Over a 2-year period, 322 water samples from the Paris area (unrelated to a legionnaires' disease outbreak) were examined for the presence of Legionella. No Legionella strain was isolated from the main water supplies of the municipality or from the distribution entry points of 36 buildings. Inside buildings, however, 84/190 (44.2%) tap water samples yielded one or several strains of Legionella (10(2) to 10(6) CFU/l). Contamination was significantly more frequent in hot tap water than in cold tap water. Legionella was isolated from 11 out of 14 air-conditioning systems investigated. Sixty-four of the 132 strains isolated were L. pneumophila serogroup 1. Other strains were L. pneumophila serogroups 2 to 6 (including a serogroup 4-5 variant), L. longbeachae serogroup 2 and 6 atypical Legionella.

Air Conditioning↗
PubMed1983

Epidemiological features of Legionnaires' disease in the Paris area.

Although the first French case of Legionnaires' disease (LD) was diagnosed in 1979 and the first six serogroups of L. pneumophila and L. longbeachae serogroup 2 were demonstrated in environmental samples from the Paris area, the incidence of the disease has not yet been evaluated in France. Prevalence of antibodies, as detected by an immunofluorescent assay, in the Paris population of our study ranged from 0.9 to 2.2%. LD was demonstrated in 155 (4.4%) of 3502 patients investigated mainly by serology. In a prospective study in a respiratory intensive care unit 20 (7.8%) cases of pneumonia out of 257 were caused by L. pneumophila. 171 of the 175 cases of LD diagnosed in Paris during 33 months were caused by L. pneumophila serogroup 1. Risk factors and epidemiological features of patients are similar to those described in other countries.

Adult↗