Search PubMed⌕ Search

Biomedical subjects

N D Goldman

Publications and source records attributed to N D Goldman.

22 records · Page 2Linked to original sources
PubMed1985

Quality control of biologicals produced by r-DNA technology.

This manuscript provides suggestions for monitoring the safety, purity, and potency of new drugs and biologics produced by recombinant DNA technology. We discussed: the Expression System, the Master Cell Bank, Production Procedure, Purification Procedure, and Characterization of the Product. These points represent our current concensus of opinion relating to the safe use of recombinant DNA products and should not be regarded as fixed or all-inclusive.

Amino Acid Sequence↗
PubMed1979

Functional similarity between the early antigens of simian virus 40 and human papovavirus BK.

The functional properties of the early antigens of simian virus 40 (SV40) and human papovavirus BK (BKV) were investigated. Infection of African green monkey kidney cells with BKV permitted the bidirectional replication of an early temperature-sensitive mutant (tsA) at a nonpermissive temperature. Conceivably, an early gene product (T-antigen) of BKV can substitute functionally for the defective SV40 T-antigen. On the other hand, SV40 DNA replication remained undetectable in human embryonic kidney cells preinfected with BKV, suggesting that BKV early antigens alone are not sufficient to provide for the replication of SV40. Preinfection of African green monkey kidney cells with BKV restored the normal pattern of late lytic SV40 transcription, suppressing the overproduction of early RNA by an SV40 tsA mutant at the nonpermissive temperature. Furthermore, preinfection of African green monkey kidney cells with BKV supported the growth of adenovirus type 2, providing a "helper function" similar to that provided by SV40 for the growth of human adenovirus in monkey kidney cells.

Animals↗
PubMedDate not supplied

Malar augmentation with self-drilling single-screw fixation.

Augmentation with malar implants has traditionally relied on fixation techniques such as creation of a precise pocket, external taping, and transcutaneous fixation techniques with bolsters. We present an alternate technique that fixates the implants to the rigid facial skeleton rather than to the dynamic active facial soft tissues. To avoid the use of expensive drills, we describe use of self-drilling screws for fixation of malar implants. The technique avoids transconjunctival incisions, allows wide exposure, and is presented as an alternative to traditional fixation techniques.

Bone Screws↗