[Aortic arch syndromes--pathological sites and symptoms].
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Adult↗
Biomedical subjects
N Chin
Publications and source records attributed to N Chin.
[Angina pectoris: classification of angina pectoris].
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Angina Pectoris↗
Direct detection and amplification of Helicobacter pylori ribosomal 16S gene segments from gastric endoscopic biopsies.
Helicobacter pylori is an organism thought to play an important causative role in gastritis and peptic ulcer diseases. We have designed an RNA dot blot assay for the detection of H. pylori, using as probe a synthetic oligonucleotide complementary to its 16S rRNA. We have also used oligonucleotide primers, complementary to conserved sequences within bacterial ribosomal 16S genes, to amplify a H. pylori ribosomal 16S DNA fragment via the polymerase chain reaction (PCR). After determining the DNA sequence of this amplified H. pylori fragment, primers were designed for specific PCR amplification of H. pylori ribosomal 16S DNA sequences. Samples from clinical endoscopic biopsies were PCR amplified with universal 16S ribosomal primers to detect the presence of bacteria and with H. pylori-specific primers to uniquely detect H. pylori. Finally, by comparing the H. pylori-specific PCR assay to commonly used diagnostic tests, we demonstrate that the molecular technique of PCR amplification shows promising applications for the clinical detection of H. pylori.
Base Sequence↗
Measurement of plasma heparin levels using a fluorometric assay.
The performance of a fluorometric heparin assay system was evaluated. The system indirectly measures heparin by its inhibition of thrombin protease action on a synthetic substrate. Our results confirm that within the range of heparin concentrations recommended by the manufacturer, i.e., 0.1-0.8 units/ml, there is an excellent correlation between expected and observed values. Within-day coefficients of variation range from 1% to 2.6%; the between-day coefficient of variation is 6.5. The accuracy of the assay diminishes under 0.1 units/ml. The assay is not influenced by bilirubin, hemolysis, fibrin split products, or lipemia. Results can be generated in less than 5 min. Possible drawbacks are the wide range of acceptability for the supplied heparin standard, and the frequency of pipetting involved.
Fluorometry↗