Biomedical subjects
Publications and source records attributed to N C Sharma.
A total of 637 strains of Salmonella bareilly received from different parts of India between 1959 and 1985 were phage typed using five locally isolated wild phages. The overall typability was 94.5% and 11 different phage types could be defined. Phage types 10 and 1 were the most prevalent and the geographical and source distribution is described.
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A new phage typing scheme using wild bacteriophages isolated from sewage for phage typing Salmonella bareilly is described. Six hundred and thirty-seven strains of Salm. bareilly could be separated into 11 different phage types using five wild phages. Overall typability was 94.5%. These phages belonged to two different morphotypes. A1 and B1, and showed varying host range.
Previous studies have demonstrated the control of fertility in monkeys on immunization with the beta subunit of ovine luteinizing hormone (beta-oLH) along with Freund's complete adjuvant (CFA). An eventual birth control vaccine would demand immunization without CFA. Beta-oLH linked to tetanus toxoid (TT) adsorbed on alum-induced antibody response in bonnet monkeys with detoxified sodium phthalyl derivative of Salmonella enteritidis lipopolysaccharide added only in the first injection. The antibodies reacted with both LH and human chorionic gonadotropin (hCG). A higher immune response was obtained with the use of a conjugate of beta-oLH and beta-hCG linked to a common carrier (TT) or with a mixture of beta-hCG/TT and beta-oLH/TT. In both cases, the antibodies were of high affinity with Ka ranging from 5 X 10(9) to 6 X 10(10) M-1. The antibodies were devoid of reactivity with human thyroid-stimulating hormone and human follicle-stimulating hormone. Monkeys with titers above 120 ng/ml hCG binding capacity (greater than 60 ng/ml in most cases) were protected from becoming pregnant during repeated mating with males of proven fertility in ovulatory cycles.
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A phage-typing system of Salmonella bareilly based on lysogeny is presented. In the present study which comprised 378 strains, fifteen different phage types named 'a' through 'o' were established. With this system 70.3% of the strains could be typed by using five indicator strains.
Macromolecular albumin-IgG complexes have been isolated from human serum and characterized immunologically. These complexes are shown to be present in plasma also and appear to be normal constituents of blood. The complexes can be demonstrated in normal serum and plasma by immunoelectrophoresis and rocket immunoelectrophoresis, as well as by use of a two-step radioimmunologic binding test. The albumin-IgG complexes inhibit adhesion of human platelets to glass and account for 12-16% of the total protein in serum.
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A calcium-binding ribonucleoprotein, specific to brain, has been shown to increase in quantity with the increase in the age of chick embryo, attaining a plateau around day 12. The increase has a correlation with the onset and maturation of the electrical activity in the developing chick embryo brain.
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The immunogenicity of the conjugate prepared from "processed" beta-subunit of human chorionic gonadotropin (choriogonadotropin, HCG) and tetanus toxoid has been studied in animals and a human subject. The conjugate elicited the formation of high-affinity (Ka = 10(9)-10(11) M-1) anti-HCG and anti-tetanus antibodies. On primary immunization, the antibody, response lasted for several months. Repeat injection of the conjugate in the declining phase of antibody titers produced a booster response without a lag period. The antibodies reacted with the beta-subunit of HCG and the complete HCG molecule but were devois of significant crossreactivity with human growth hormone, placental lactogen, follicle-stimulating hormone, thyroid-stimulating hormone, and luteinizing hormone at tonic and surge levels. The antibodies were competent for neutralizing the biological activity of HCG in the mouse uterine weight gain assay, the ventral prostate weight gain assay, and the radioligand assay for binding of 125I-labeled HCG to receptors on corpus luteum. HCG (5000 international units) administered to an immunized subject was completely bound by circulating antibodies. Administration of HCG (in contrast to conjugate) was without booster effect on anti-HCG titers.