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Biomedical subjects

N Burns

Publications and source records attributed to N Burns.

34 records · Page 2Linked to original sources
PubMed1983

Production and characterization of monoclonal antibodies against avian retrovirus reverse transcriptase.

Monoclonal antibodies were prepared against the avian myeloblastosis virus reverse transcriptase. These monoclonal antibodies specifically immunoprecipitated the alpha and beta subunits of the reverse transcriptase molecule, as well as the Pr180gag-pol precursor protein present in virus-infected cells. In addition, these monoclonal antibodies inhibited the DNA polymerase activity associated with the reverse transcriptase molecule but not the RNase H activity. The monoclonal antibody preparations were specific for the amino-terminal portion of the protein, as determined by the immunoprecipitation of a reverse transcriptase-beta-galactosidase fusion protein produced in Escherichia coli by molecular cloning procedures.

Animals↗
PubMed1978

Early vascular changes in the spinal grey matter following impact injury.

The ultrastructural changes of the microvasculature in the spinal grey matter were studied after impact injuries of varying severity in cats. Survival times ranged from 3 min to 6 h after injuries. The main vessels affected were capillaries and postcapillary venules. Perivascular haemorrhage and protein extravasation were present immediately after impact. Astrocytic foot processes could be swollen or shrunken and of increased electron density. The extra cellular space (ECS) was often distended with proteinaceous material. Some vessels showed an apparent increase in vesicles and small dense inclusions were seen in pericytes. By 20--30 min after impact, a number of vessels at greater impact energies showed degenerative changes in organelles and endothelial gaps. The lumina of these vessels often contained plasma, platelets, red cells and occasional neutrophils. Similar changes were seen between 3 and 6 h and at this stage neutrophils and some monocytes were present in the perivascular spaces and neuropil. Endothelial balloons and pericytic dense inclusions were also seen. It is suggested that initial events are mechanical, perhaps due to a rapid displacement of blood in venules followed by arterial hypertension (which commonly accompanies spinal cord impacts). Although initially many lumena are patent by 30 min there is a difference between the lesser and greater severities of impact.

Animals↗
PubMedDate not supplied

Economics of hospice care.

As an institution providing care for patients who are dying, hospice has expanded significantly since the opening of the first hospice in the United States in 1974. Many changes have occurred in that time, including the maturation of hospices and the introduction of third-party reimbursement under Medicare. This article examines literature on hospice from an economic perspective; the focus is on the characteristics of the hospice "industry," demand for hospice care, hospice as a supplier of care, and the cost of hospice care. Two major gaps in the research are the failure to categorize hospices by the type of reimbursement received and the emphasis on expenditures for care to the exclusion of consideration of the cost of producing hospice care.

Costs and Cost Analysis↗
PubMedDate not supplied

Hospice costs and medicare reimbursement: an application of break-even analysis.

The adequacy of Medicare reimbursement to cover hospice costs is examined using break-even analysis. Since hospice costs are high during the first few days after enrollment, a long period of enrollment is required for per diem reimbursement to cover costs. Under a variety of specifications, the length of stay required for the hospice to break even is greater than the median length of stay.

Aged↗