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Biomedical subjects

N B Williamson

Publications and source records attributed to N B Williamson.

At least 37 records · Page 2Linked to original sources
PubMed1996

Effect of selenium and a-tocopherol supplementation on postpartum reproductive function of dairy heifers at pasture.

The objective of this study was to investigate the effects of selenium (Se) and alpha-tocopherol supplementation on uterine involution and ovarian function in dairy heifers fed a prepartum diet containing low concentrations of Se and alpha-tocopherol. Twenty-four pregnant Friesian heifers were randomly allocated to one of four experimental groups in a 2 x 2 design balanced for age and body weight. Prepartum treatments consisted of supplementation with either 2 intraruminal Se pellets or 3600 mg of alpha-tocopherol p.o. 4 times per wk, or both. Control animals received no supplementation. For 8 wk before calving, the heifers were fed exclusively on pasture hay which contained less than 10 microg/kg of Se and 19 mg/kg of alpha-tocopherol. After calving, the heifers grazed perennial ryegrass and white clover pasture. Concentrations of Se and alpha-tocopherol in serum for the prepartum heifers of the control group were 10 ng/ml and 1.3 microg/ml, respectively, indicating deficiencies of these nutrients. Treatment with Se and alpha-tocopherol increased prepartum serum concentrations of Se and alpha-tocopherol to 74 ng/ml and 5 microg/ml, respectively (P < 0.001). However, treatment with Se, alpha-tocopherol, or both, failed to enhance uterine involution, hasten resumption of postpartum ovarian activity or reduce the incidence of clinical postpartum abnormalities. These findings suggest that postpartum reproductive dysfunction is not a primary feature of moderate Se or vitamin E deficiency of cattle at pasture.

Journal Article↗
PubMed1996

Alpha-tocopherol, selenium and polyunsaturated fatty acid concentrations in the serum and feed of spring-calving dairy heifers.

The objectives of this study were to provide baseline data for alpha-tocopherol, selenium and polyunsaturated fatty acid concentrations in the serum and feed of New Zealand dairy cattle, and to assess the likelihood that abnormal peroxide metabolism has a role in the impaired lactational and reproductive performance noted in selenium-deficient cattle. Twenty-four Friesian heifers were randomly allocated one of four winter diets consisting of hay with or without selenium supplementation, or pasture and silage with or without selenium supplementation. A winter diet consisting exclusively of hay (alpha-tocopherol concentration 19 mg/kg of dry matter) resulted in a pre-calving serum alpha-tocopherol concentration of 1.2 mg/l compared to 4.5 mg/l for pastured heifers (p<0.01). The pre-calving alpha-tocopherol concentration for the heifers fed hay fell into the range considered deficient (<2.0 mg/l), whereas heifers fed pasture and silage remained in the range considered adequate throughout the study period. Serum fatty acid concentration, and the proportion of fatty acids that were polyunsaturated, were lowest in the hay-fed heifers before calving (1.0 mg/ml, 37.1% respectively), and remained unchanged following re-introduction to pasture after calving in late July and August. Serum fatty acid concentration did not increase following the re-introduction of the heifers to pasture because of the unexpectedly low fatty acid concentration (4.8 g/kg of dry matter) of the mature winter pasture. In October, however, the proportion of fatty acids in serum that were polyunsaturated increased (50%) as did serum cy-tocopherol concentrations (greater than 13 mg/l). Mean serum selenium concentrations in the unsupplemented heifers ranged from 139 to 204 nmol/l, being lowest in October (p<0.01). Supplementation with intraruminal selenium pellets (two pellets delivering about 3 mg of selenium/day) increased serum selenium concentration and glutathione peroxidase activity (p<0.01) whereas the type of winter diet had no effect (p>0.05). These results suggest that dairy cattle wintered on hay can become Vitamin E-depleted, whereas the feeding of pasture and silage should provide adequate Vitamin E. The pasture offered following calving during July and August provided a low dietary polyunsaturated fatty acid challenge, suggesting that abnormal peroxide metabolism is unlikely to be an important mechanism in the impaired performance of selenium-deficient adult cattle which calve at this the of year.

Journal Article↗
PubMed1996

Isocupressic acid, an abortifacient component of Cupressus macrocarpa.

Anecdotal reports suggest that abortion occurs in New Zealand cattle after ingestion of macrocarpa (Cupressus macrocarpa). The clinical signs observed are similar to those seen in abortions caused by ponderosa pine (Pinus ponderosa Laws). Isocupressic acid has been identified as an abortifacient component of the needles of ponderosa pine. Macrocarpa collected from the Palmerston North area contained 0.89% and 1.24% isocupressic acid, compared to 1.70% isocupressic acid in Ponderosa pine needles from Oregon in the United States as analysed by the USDA-ARS Poisonous Plant Research Laboratory. These findings support observations that macrocarpa causes abortion in late gestation cattle in New Zealand and suggest the causal agent is probably isocupressic acid.

Journal Article↗
PubMed1996

Serum glutathione peroxidase activity reflects short-term increases in selenium intake in goats.

The objective of this study was to determine if serum glutathione peroxidase activity reflects short-term changes in the selenium status of goats. Angora goat kids (n=14) were fed pelleted luceme containing 20 microg/kg of selenium, and treated orally with either selenium (0.1 mg/kg of liveweight weekly, as sodium selenate) or de-ionised water. Serum activity of glutathione peroxidase was increased in response to supplementation and differed from that of controls within 24 hours of supplementation. The change in serum glutathione peroxidase activity during the 21 days after the start of weekly supplementation closely followed changes in serum selenium concentration. The results of this study suggest that serum glutathione peroxidase activity reflects the short-term improvement in the selenium status of Angora goat kids following oral supplementation with sodium selenate.

Journal Article↗
PubMed1996

Effect of intraruminal monensin capsules on reproductive performance and milk production of dairy cows fed pasture.

Cows from three herds calving in spring that were fed on pasture were paired by herd, production index, predicted calving date, and age. One cow from each pair was allocated randomly to the treatment group and received a controlled-release capsule of monensin 1 mo prior to the start of AI. A random sample of 20 matched pairs of cows was selected within each herd to monitor BW and metabolic parameters. Treated cows produced more fat, protein, and liters of milk per day during the 2nd mo of monensin activity and produced a greater total milk volume than did control cows. Treated cows gained less BW during the 1st mo after treatment. No significant differences were identified in submission rates, overall pregnancy rates, or pregnancy rate to first or second AI. Blood urea nitrogen was elevated in the treatment group as a main effect, and NEFA were elevated in the 2nd and 3rd mo after treatment. There was no significant effect on blood concentrations of glucose, albumin, or BHBA.

3-Hydroxybutyric Acid↗
PubMed1996

Effect of selenium and lodine supplementation on growth rate and on thyroid and somatotropic function in dairy calves at pasture.

The effects of Se and I supplementation on growth rate and on thyroid and somatotropic function were examined for heifer calves from two herds fed pasture. Supplementation of calves with intraruminal Se pellets increased the basal plasma concentration of 3,5,3'-triiodothyronine and reduced the basal plasma concentration of thyroxine for both herds. For one herd, supplementation with Se increased the triiodothyronine response to challenge with thyrotropin-releasing hormone, increased BW gain, and tended to increase the plasma concentration of IGF-I. The plasma concentration of growth hormone was unaffected by Se supplementation. Supplementation with I increased the response of thyroid hormones to thyrotropin-releasing hormone but did not increase BW gain. Interaction between Se and I treatment within the herds was not apparent for any outcome variable. These data suggest that the effects of Se deficiency in grazing calves may be mediated by alterations in thyroid hormone metabolism but apparently are not mediated through modulation of the peripheral concentration of growth hormone.

Animals↗
PubMed1995

Some risk factors associated with clinical lameness in dairy herds in Minnesota and Wisconsin.

An observational study of the relationships between clinical lameness and herd-level risk factors was made in 18 dairy herds in Minnesota and Wisconsin. Lameness in the lactating cows was assessed by two observers during herd visits made in the summer of 1989 and the spring of 1990, using a uniform scoring system. A questionnaire was used to obtain information about the general management, routine hoof care, nutrition, housing and flooring on the farms. Factors associated with the prevalence of clinical lameness in the summer included stall moisture, the size of the exercise area for the lactating cows and the amounts of dry concentrates and fresh forages fed to them. Factors associated with clinical lameness in the spring included the use of parlour milking facilities and the frequency with which the rations for the lactating cows were balanced.

Animal Feed↗
PubMed1995

Factors associated with use of prostaglandins in reproductive herd health programs for dairy cows.

Use of prostaglandin F2 alpha products (PG) in dairy cows was studied by use of computerized reproductive herd health data compiled from 29 dairy herds in Minnesota and Wisconsin during 1983 through 1988. Treatment with PG was recorded in 1,856 of 7,139 (26.0%) complete lactations. More than 1 treatment with PG was administered in 534 of the 1,856 (28.8%) lactations in which PG were used. The proportion of lactations in which treatment was used varied among herds and years, and was associated with the proportion of cows in which natural breeding was attempted and, to a lesser extent, with estrus detection index and conception rates; these associations were potentially modified by time between veterinary herd health visits. Calving-to-treatment intervals (mean +/- SD) were 100.4 +/- 55.1 days for first treatments only, and 116.2 +/- 66.1 days for all treatments. These values varied considerably among herds and years and among reasons for treatment. Much of the variation in proportions of lactations in which treatment was used and in the time of treatment within lactations could not be accounted for with the variables included in this study, and was probably related to individual considerations for each cow.

Animals↗
PubMed1995

Patterns of follicular development during periods of anovulation in pasture-fed dairy cows after calving.

The ovaries of 17 Friesian cows aged two to seven years were examined daily by transrectal ultrasound from one week after calving. The position and size of all follicles more than 2 mm in diameter were recorded. A large (more than 9 mm in diameter) follicle was present on at least one ovary by six to 17 days after calving, and in two of the cows it ovulated; the remaining cows had from two to nine large follicles before they ovulated. The mean (SEM) interval from calving to the first ovulation was 43.4 (5.3) (range 13 to 93) days, after 4.2 (0.6) waves of follicles.

Animal Feed↗
PubMed1995

Some effects of feeding pasture silage as a supplement to pasture on reproductive performance in lactating dairy cows.

Supplementation of a ryegrass/white clover pasture diet with pasture silage is a common management practice in New Zealand dairy herds. The effect of this supplementation on reproductive performance has not been investigated. Five herds of 20 cows were formed before calving commenced on 1 June 1992. From 5 August to 4 September, two of these herds were fed 5 kg/cow/day of pasture silage in addition to the ryegrass/white clover pasture offered to all herds. Pasture silage supplementation did not alter the intervals from calving to first ovulation, first oestrus, or to conception. However, it reduced the first service conception rate (37.5% v. 53.3%; difference +/- sed = 15.8 +/- 10.0%, for pasture and silage and pasture only cows, respectively). A positive effect on reproductive performance of pasture silage supplementation was not demonstrated in this trial.

Journal Article↗
PubMed1994

The effect of intra-ruminal selenium pellets on growth rate, lactation and reproductive efficiency in dairy cattle.

In each of two dairy herds (A and B), rising yearling heifers (Trial 1) and adult cows (Trial 2) were assigned to three treatment groups. Untreated animals were compared to animals treated with either two or four intra-ruminal pellets containing 3 g of elemental selenium. The administration of pellets at the recommended dose (two pellets per animal) was effective in elevating whole blood glutathione peroxidase activity and selenium concentration to over 10 times those of control animals. In Trial 1, a 15% response in liveweight gain (p<0.001) occurred in yearling heifers in the herd with the lowest pre-treatment selenium status. In Trial 2, cows receiving two pellets produced a greater milk volume (p=0.06) and more milk solids (p=0.02) than untreated controls; an increase in volume of 5.4% and 8%, and in milk solids of 6.5% and 6.4%, were noted in herds A and B respectively. There was a trend towards decreasing somatic cell counts in milk from the treated cows when compared to controls, the four-pellet group in Herd A and the two-pellet group in Herd B being significantly different from their respective control group. No between-group differences were noted in calving-first service or calving-conception intervals, nor in the proportion of animals pregnant to first or all services. The administration of selenium at twice the recommended dose rate yielded no additional response above that noted after the administration of the recommended dose. The results of this study support the use of currently recommended Ministry of Agriculture and Fisheries selenium reference ranges in cattle for the prediction of a response to supplementation.

Journal Article↗
PubMed1991

A longitudinal study of the hooves of non-lame cows.

A longitudinal study of the feet of cows from a seasonal dairy herd was conducted over a 12 month period to measure the occurrence of subclinical hoof lesions. Eleven 2-year-old cows, entering the herd for the first time, and eleven mature cows were randomly selected from a herd of 415 cows at the end of winter (July 1989) prior to calving. The incidence of lameness in the herd over the 12 months was 2%. None of the trial cows became lame during the study. Cows were examined monthly. The type, severity and location of any hoof lesions were recorded. Sole haemorrhage, erosion of the heel bulb and minor white line separation were the most commonly observed lesions. These lesions were observed with specific claw distributions in the cow and at specific times of the year and breeding season, with waves of each type of lesion passing through the herd at a particular time. White line separation was observed commonly during spring (up to 37% of digits affected), disappeared almost completely over the dry summer months and reappeared in autumn, increasing to 40%' again over winter. Lesions were more commonly observed in the lateral digits, with fore feet being more commonly affected than hind feet. Erosion of the heel bulbs was common in August (65% of digits affected, more common in mature cows than 2-year-olds), disappeared quickly during spring, and reappeared in all cows during the following winter (88% of digits; no age differences). There were no differences in distribution between digits. Sole haemorrhages were concentrated over the mid-sole and abaxial sole zones. They were more common in the hind feet than the front feet and more common in the outside claws (p < 0.001). Haemorrhages were not observed prior to or soon after calving, appeared in October and reached a peak of 40% in December, then gradually disappeared by the time of drying-off in May. The 2-year-olds were more frequently affected than the mature cows (p <0.001).

Journal Article↗
PubMed1990

The DairyCHAMP program: a computerised recording system for dairy herds.

The DairyCHAMP program is an animal health and management software program that helps daily animal management, herd performance monitoring and problem analysis. Data entry to the program uses a data dictionary and includes an error-checking system that ensures the consistency and appropriateness of data entered. DairyCHAMP performs health management functions, provides a convenient user interface, ensures uniform data across farms by using a standard data dictionary, can be fully integrated with decision-making software programs like DairyORACLE, and is flexible enough to be useful for many types of dairy facilities. Data are entered via a menu-based system. Animal events are organised around reproduction and lactation cycles and health records. Farm records include inventories for drug, feed and semen. Farm parameters can be established which customize the program for an individual farm. The database system is an integration of three schemas: the individual user's view, the community view and the storage system. The individual user's view must be easy to use, while the storage system must be compact enough to fit within the disc storage space on a microcomputer. This conflict requires a translation from one schema to another. The DairyCHAMP program accomplishes this through a coding system which assigns a code number to each event. The program can add synonyms to this event dictionary by assigning the same code number to the synonym the user chooses. The DairyCHAMP program provides access to the large amounts of data required to aid in daily animal management, allow performance monitoring and analyse problems. Its highly integrated system is efficient and easy to use and maintain.

Animal Husbandry↗
PubMed1987

Seasonal influences on conception efficiency in Minnesota dairy herds.

This study was undertaken to investigate the influence of climatological factors on the conception efficiency of cows in 23 Minnesota dairy herds between March 1982 and March 1985. It was found that year of insemination (P < 0.005), herd (P < 0.005), linear and quadratic terms of days open (P < 0.05), and linear and quadratic terms of minimum temperature (MNT), maximum temperature (MXT) and temperature humidity index (THI) were similarly related to observed monthly variations in first service conception rates. The year of insemination (P < 0.005), herd (P < 0.005), and linear and quadratic terms of MNT, MXT and THI were also related to the variation in overall conception rate. Conception rate declined from April to August and increased from August to January. There was an 11% difference between the month of lowest fertility (August) and the month of highest fertility (November). It can be concluded that climatological factors appear to depress herd fertility in the northern United States as well as in the warmer southern areas. Thus, consideration should be given to strategies which will minimize the impact of the observed fertility depression due to these climatic factors.

Journal Article↗
PubMed1985

The influence of some sample handling factors on progesterone and testosterone analysis in goats.

This study validated the use of commercially available radioimmunoassay kits for measuring the circulating progesterone and testosterone levels of goats. Progesterone and testosterone levels were then assayed in plasma which was collected from 23 does and 8 bucks. Collections from each animal were divided into three sodium fluoride-potassium oxalate (F/OX), one heparin, and one EDTA tubes and also into a tube without anticoagulant. Plasma from an F/OX tube was separated immediately from the blood cells by centrifugation. Serum or plasma was also separated after storage for 24 hours with F/OX, heparin or EDTA anticoagulant at 22 degrees C or with F/OX at 5 degrees C. A significant decline in assayable progesterone occurred in samples stored at 22 degrees C with each anticoagulant used and in the serum sample. Samples stored at 5 degrees C for 24 hours with F/OX anticoagulant contained concentrations of progesterone which did not differ significantly from those in samples where plasma was removed immediately. Assayable testosterone did not change with the anticoagulant used or vary with the storage temperature when F/OX tubes were stored at 5 degrees C and 22 degrees C for 24 hours. Results indicate that sample storage does influence levels of measured progesterone but not testosterone in goats. Progesterone assay is best done on plasma which is immediately separated from blood cells or on samples which are stored at 5 degrees C.

Journal Article↗
PubMed1983

Effects of intrauterine challenge with Leptospira interrogans serovar hardjo on fertility in cattle.

The purpose of this study was to determine the effects of Leptospira interrogans serovar hardio on fertility in cattle. Twenty seronegative mature dairy cows were assigned to two groups. Group I (challenged cows) was bred by a seronegative bull followed by intrauterine infusion (within 30 minutes) of Leptospira interrogans serovar hardjo. Group II was bred by the same bull followed by intrauterine infusion of 5 ml of sterile culture medium. Blood samples were collected at two-day intervals to monitor serum antibody titers. Daily blood cultures for 10 days and weekly urine cultures for five weeks were performed to monitor the animals for leptospiremia and leptospiuria. Cows were slaughtered 35 days post-breeding, and their reproductive tracts were examined. All animals remained clinically normal following intrauterine challenge. There was no difference in pregnancy rates (Group I, 7/10; Group II, 6/10). All embryos, reproductive tracts, and kidneys appeared normal. A microscopic agglutination test (MA) showed that 4 of 10 challenged cows developed serum antibody titers between 8 and 20 days after challenge. However, on the basis of the hamster passive protection test, all challenged cows had serum antibodies present. All blood and urine cultures were negative through the experimental period, as were the final kidney and uterine cultures. In a second experiment, six seronegative cows were infused with killed microorganisms immediately after insemination. Results of a microscopic agglutination test and a hamster passive protection test indicated that these cows did not develop humoral antibodies against serovar hardjo. These results indicated that intrauterine inoculation of Leptospira interrogans serovar hardjo (hamster-adapted strain) following breeding did not affect pregnancy rates despite an intrauterine challenge which caused the development of humoral antibodies.

Journal Article↗