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Biomedical subjects

N Ackerman

Publications and source records attributed to N Ackerman.

At least 37 records · Page 2Linked to original sources
PubMed1987

Chronic urinary tract infection in dogs: induction by inoculation with bacteria via percutaneous nephropyelostomy.

Dogs were inoculated via percutaneous nephropyelostomy with bacteria isolated from canine patients with urinary tract infections (4 dogs were inoculated with Escherichia coli, 2 were inoculated with Proteus mirabilis, and 1 was inoculated with coagulase-positive staphylococci). At approximately monthly intervals after bacterial inoculation, bladder urine specimens were collected via antepubic cystocentesis, and renal pelvic urine specimens were collected via percutaneous nephropyelocentesis. Dogs were euthanatized between 89 and 294 days after bacterial inoculation. Extensive microscopic examination was conducted on the urinary tract of each dog. The dogs did not develop any of the common clinical signs of urinary tract infections (ie, increased frequency of urination, fever, craniodorsal abdominal [renal] pain, or malaise). Inflammation in the lower urinary tract of the dogs was more severe than that in the kidneys. Although pyelitis was present, extension into the outer medulla and renal cortex seemed to be confined to nephropyelostomy tracts. The most severe bladder lesion was found in the dog that developed cystic calculi after inoculation with coagulase-positive staphylococci.

Animals↗
PubMed1986

The effects of histamine and serotonin on polymorphonuclear leukocyte accumulation in the rat.

Histamine and serotonin were evaluated for their effects on rat polymorphonuclear leukocyte (PMN) accumulation in vivo and PMN migration in vitro. Both of the mediators inhibited the accumulation of PMNs when injected into the pleural cavity in a saline vehicle, and reduced the PMN content of the peripheral blood. Histamine also reduced the cellular influx when administered in combination with an intrapleural injection of carrageenan. Peripheral blood leukocytes removed from rats injected intrapleurally with histamine and carrageenan had a lesser chemotactic responsiveness compared with those removed from rats injected only with carrageenan. The effects of histamine in reducing PMN accumulation was abolished by treatment with cimetidine, an H2 antagonist, but not by treatment with chlorpheniramine, an H1 antagonist. These results suggest that a local release of histamine may play a role in reducing cellular infiltration into an inflammatory site.

Animals↗
PubMed1986

Chemotherapy of synovial cell sarcoma in a dog.

A synovial cell sarcoma of the tarsus in a 6-year-old dog was treated with doxorubicin HCl and cyclophosphamide. The tumor regressed after treatment. There was no recurrence 3 years after initiation of treatment. Previously, synovial cell sarcoma has not been reported to be responsive to chemotherapeutic agents, and the treatment of choice has been amputation.

Animals↗
PubMed1985

Progressive polysystemic immune-mediated disease in a dog.

A dog with polyarthritis, angular joint deformities, and a high serum antinucleolar antibody titer was treated over a period of 20 months. During the clinical course, leukopenia, thrombocytopenia, Coombs' positive hemolytic anemia, and a pemphigus-type skin disorder developed, all of which responded to immunosuppressive therapy. It is not known whether the polysystemic disease in this dog represents a pleomorphic manifestation of canine systemic lupus erythematosus or multiple autoimmune disorders occurring in the same animal.

Animals↗
PubMed1985

Blood flow in the renal portal circulation of the turkey: effect of epinephrine.

Control of blood flow in the renal portal circulation of turkeys given epinephrine was evaluated by determining the distribution of injected radiopaque contrast medium within the portal circulation. Radiopacity in the region of the renal parenchyma and/or the caudal renal portal vein was increased in each of the 6 birds after epinephrine (4.0 micrograms/kg of body weight) was injected into the wing vein. In 1 bird, the contrast medium also was distributed into the mesenteric vein. Thus, epinephrine not only induced flow of blood from the pelvic limb (leg) to the kidneys, but to the abdominal viscera as well. Regulation of the patency of the renal portal valve is thought to be one means by which renal portal blood flow is controlled. However, epinephrine had no consistent effect on the valve. Thus, epinephrine seemed to control renal portal blood flow and, perhaps, blood flow to other abdominal viscera at sites other than the renal portal valve.

Animals↗
PubMed1984

Hereditary continuous muscle fiber activity.

We describe a family with classic features of continuous muscle fiber activity (Isaacs-Mertens syndrome) appearing in an autosomal dominant pattern. Both a mother and her son had muscle stiffness and rigidity in early childhood. Because the mother's condition was not immediately apparent, we recommend a thorough examination of family members.

Adult↗
PubMed1982

Relationship between adherence, chemotaxis and the accumulation of rat polymorphonuclear leukocytes at an inflammatory site.

The effects of a nonsteroidal anti-inflammatory agent, indomethacin, a steroidal anti-inflammatory drug, dexamethasone and an antirheumatic agent, levamisole, on rat polymorphonuclear leukocyte (PMN) adherence and migration were examined. Adherence was evaluated using nylon fiber columns and migration was studied in vitro using Boyden chambers In addition, the effects of these drugs on PMN accumulation in he carrageenan-injected pleural cavity was evaluated. PMNs removed from animals treated with indomethacin, 1 mg/kg p.o., had a significantly reduced adherence, and fewer cells accumulated at an inflammatory site. Cellular migration measured in vitro was unaffected. PMNs removed from animals treated with dexamethasone, 0.1 and 1.0 mg/kg p.o., had a significantly reduced adherence and fewer cells accumulated at an inflammatory site. Cell migration was not consistently affected. After treatment with levamisole, 5 and 25 mg/kg p.o., adherence and chemotaxis were reduced, whereas PMN accumulation was unaffected. These results demonstrate that no simple relationship exists between PMN adherence, chemotaxis and their accumulation at an inflammatory site. Neither a reduction in adherence nor migration, as measured in vitro with a Boyden chamber, was predictive of a change in inflammatory cell migration.

Animals↗
PubMed1980

Thoracic and abdominal radiographic abnormalities in the multicentric form of lymphosarcoma in dogs.

Thoracic and abdominal radiographs from 100 dogs with lymphosarcoma were reviewed to determine the type and frequency of radiographic abnormalities associated with the disease. Sternal lymphadenopathy was the most frequent (60%) thoracic radiographic abnormality. Hepatomegaly (47%), splenomegaly (55%), and sublumbar lymphadenopathy (49.5%) were the most frequent abdominal radiographic abnormalities; these were most often seen in combination. A single radiographic abnormality or combination of abnormalities was not observed frequently enough to suggest that a diagnosis could be made only on the basis of radiographic findings.

Abdominal Neoplasms↗
PubMed1980

Relation of antibody-coated urine bacteria to the site(s) of infection in experimental dogs.

Antibody-coated bacteria tests, using fluorescein-conjugated rabbit anti-canine immunoglobulin (Ig)G and anti-IgA, were conducted on 77 specimens of bladder urine obtained by antepubic cystocentesis from 25 dogs with urinary tract infections. Urine specimens from 3 dogs infected with Staphylococcus aureus were tested a total of 27 times, specimens from 10 dogs infected with Escherichia coli were tested 25 times, urine from 6 dogs infected with Proteus mirabilis was sampled and tested 18 times, urine from 3 dogs with Klebsiella pneumoniae was sampled and tested 4 times, and specimens from 3 dogs infected with P rettgeri, Enterobacter cloacae, or Streptococcus faecalis were tested once each. Specimens of urine from the renal pelves of each dogs were obtained by percutaneous nephropyelocentesis at the same time as the specimens of bladder urine. The results of bacterial cultures of these specimens were compared with the results of the corresponding antibody-coated bacteria tests. False-positive results (ie, evidence of antibody coating of bladder urine bacteria but no bacterial growth from corresponding renal pelvis urine specimens) were obtained in 13 of 22 (59%) tests of dogs infected with S aureus, 12 of 13 (92%) tests in dogs infected with P mirabiis, 5 of 18(28%) tests in dogs infected with E coli, and 1 of 4 (25%) tests in dogs infected with K pneumoniae. False-negative results (ie, bacterial growth from renal pelvis urine specimens but no evidence of antibody coating of bacteria in the corresponding specimen of bladder urine) occurred in 5 of 7 (71%) specimens infected with E coli, in 4 of 5 (80%) specimens infected with P mirabilis, and in 5 of 5 (100%) specimens infected with S aureus. It was concluded that the antibody-coated bacteria test does not provide accurate information to support the diagnosis of pyelonephritis in the dog.

Animals↗
PubMed1980

Three day pleural inflammation: a new model to detect drug effects on macrophage accumulation.

Mononuclear cell accumulation is of major importance in maintaining chronic inflammatory conditions. In an effort to model this phenomenon, 0.3 ml of a 1% carrageenan solution was injected into the pleural cavity of rats; at various times thereafter peripheral blood and pleural exudate samples were collected. Seventy-two hours after carrageenan injection, 82.3 +/- 3.7 x 10(6) cells (N = 6; mean +/- S.E.) were present in the pleural cavity; over 80% of these cells were macrophages as determined by morphologic and histochemical criteria. Animals treated with dexamethasone had a significantly reduced number of pleural macrophages. Animals treated with the nonsteroidal anti-inflammatory agents, naproxen and indomethacin, had an elevated intrapleural macrophage content. The number of intrapleural cells was not affected by the antirheumatic agents levamisole, d- and dl-penicillamine or gold sodium thiomalate. Animals treated with tilorone, dapsone, hydroxychloroquine, phenylmethane-sulfonyl fluoride and 1,10 phenanthroline had a reduced pleural cell count.

Animals↗
PubMed1979

Lymphosarcoma and cryptococcosis in a cat.

Cryptococcosis and lymphosarcoma were diagnosed in a 3-year-old Domestic Shorthair cat. The cat was studied immunologically, and abnormal findings included absolute lymphopenia, with a low percentage of the lymphocytes having cell surface markers of normal T and B cells, decreased responsiveness to mitogens, and low immunoglobulin concentrations in serum. Necropsy revealed cryptococcal lesions in the facial area. The cortex of the left kidney was replaced by a diffuse pattern of undifferentiated large cells compatible with those of histiocytic cell type lymphosarcoma.

Animals↗
PubMed1979

Release of cartilage proteoglycan degrading enzyme activity by thioglycollate stimulated mouse peritoneal macrophages in culture.

Media from cultured mouse peritoneal macrophages were tested for cartilage proteoglycan degrading activity using S35-labelled rabbit ear cartilage. Media samples collected at 2-day intervals contained increasing amounts of activity between days two and six. This activity was activated by trypsin and antagonized by chelating agents. The macrophage products induced release of the proteoglycan component of cartilage as determined by biochemical and histological methods without affecting the collagen component. Media from cells incubated with hydrocortisone were devoid of proteoglycan degrading activity.

Animals↗